Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain
Yong Fang Zhu,1,2 Peter Kan,3 Gurmit Singh1,2 1Michael G. DeGroote Institute for Pain Research and Care, McMaster University, Hamilton, ON, Canada; 2Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada; 3Health Sciences, McMaster University, Hamilton, ON, Canad...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-10-01
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| Series: | Journal of Pain Research |
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| Online Access: | https://www.dovepress.com/differences-and-similarities-in-spontaneous-activity-between-animal-mo-peer-reviewed-fulltext-article-JPR |
| _version_ | 1811182347442192384 |
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| author | Zhu YF Kan P Singh G |
| author_facet | Zhu YF Kan P Singh G |
| author_sort | Zhu YF |
| collection | DOAJ |
| description | Yong Fang Zhu,1,2 Peter Kan,3 Gurmit Singh1,2 1Michael G. DeGroote Institute for Pain Research and Care, McMaster University, Hamilton, ON, Canada; 2Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada; 3Health Sciences, McMaster University, Hamilton, ON, CanadaCorrespondence: Gurmit Singh, Email Singhg@mcmaster.caBackground: Clinical data on cancer-induced pain (CIP) demonstrate widespread changes in sensory function. It is characterized in humans not only by stimulus-invoked pain, but also by spontaneous pain. In our previous studies in an animal model of CIP, we observed changes in intrinsic membrane properties and excitability of dorsal root ganglion (DRG) sensory neurons corresponding to mechanical allodynia and hyperalgesia, of which abnormal activities of Aβ-fiber sensory neurons are consistent in a rat model of peripheral neuropathic pain (NEP).Objective: To investigate whether there are related peripheral neural mechanisms between the CIP and NEP models of spontaneous pain, we compared the electrophysiological properties of DRG sensory neurons at 2– 3 weeks after CIP and NEP model induction.Methods: CIP models were induced with metastasis tumour-1 rat breast cancer cells implanted into the distal epiphysis of the femur. NEP models were induced with a polyethylene cuff implanted around the sciatic nerve. Spontaneous pain in animals is measured by spontaneous foot lifting (SFL). After measurement of SFL, the animals were prepared for electrophysiological recordings of spontaneous activity (SA) in DRG neurons in vivo.Results: Our data showed that SFL and SA occurred in both models. The proportion of SFL and SA of C-fiber sensory neurons in CIP was more significantly increased than in NEP models. There was no difference in duration of SFL and the rate of SA between the two models. The duration of SFL is related to the rate of SA in C-fiber in both models.Conclusion: Thus, SFL may result from SA activity in C-fiber neurons in CIP and NEP rats. The differences and similarities in spontaneous pain between CIP and NEP rats is related to the proportion and rate of SA in C-fibers, respectively.Keywords: electrophysiology recording, spontaneous pain, sensory neuron, cancer-induced pain, neuropathic pain |
| first_indexed | 2024-04-11T09:31:00Z |
| format | Article |
| id | doaj.art-2a92a2b691744c648f1391acd127c4c1 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-04-11T09:31:00Z |
| publishDate | 2022-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Pain Research |
| spelling | doaj.art-2a92a2b691744c648f1391acd127c4c12022-12-22T04:31:53ZengDove Medical PressJournal of Pain Research1178-70902022-10-01Volume 153179318778901Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic PainZhu YFKan PSingh GYong Fang Zhu,1,2 Peter Kan,3 Gurmit Singh1,2 1Michael G. DeGroote Institute for Pain Research and Care, McMaster University, Hamilton, ON, Canada; 2Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada; 3Health Sciences, McMaster University, Hamilton, ON, CanadaCorrespondence: Gurmit Singh, Email Singhg@mcmaster.caBackground: Clinical data on cancer-induced pain (CIP) demonstrate widespread changes in sensory function. It is characterized in humans not only by stimulus-invoked pain, but also by spontaneous pain. In our previous studies in an animal model of CIP, we observed changes in intrinsic membrane properties and excitability of dorsal root ganglion (DRG) sensory neurons corresponding to mechanical allodynia and hyperalgesia, of which abnormal activities of Aβ-fiber sensory neurons are consistent in a rat model of peripheral neuropathic pain (NEP).Objective: To investigate whether there are related peripheral neural mechanisms between the CIP and NEP models of spontaneous pain, we compared the electrophysiological properties of DRG sensory neurons at 2– 3 weeks after CIP and NEP model induction.Methods: CIP models were induced with metastasis tumour-1 rat breast cancer cells implanted into the distal epiphysis of the femur. NEP models were induced with a polyethylene cuff implanted around the sciatic nerve. Spontaneous pain in animals is measured by spontaneous foot lifting (SFL). After measurement of SFL, the animals were prepared for electrophysiological recordings of spontaneous activity (SA) in DRG neurons in vivo.Results: Our data showed that SFL and SA occurred in both models. The proportion of SFL and SA of C-fiber sensory neurons in CIP was more significantly increased than in NEP models. There was no difference in duration of SFL and the rate of SA between the two models. The duration of SFL is related to the rate of SA in C-fiber in both models.Conclusion: Thus, SFL may result from SA activity in C-fiber neurons in CIP and NEP rats. The differences and similarities in spontaneous pain between CIP and NEP rats is related to the proportion and rate of SA in C-fibers, respectively.Keywords: electrophysiology recording, spontaneous pain, sensory neuron, cancer-induced pain, neuropathic painhttps://www.dovepress.com/differences-and-similarities-in-spontaneous-activity-between-animal-mo-peer-reviewed-fulltext-article-JPRelectrophysiology recordingspontaneous painsensory neuroncancer-induced painneuropathic pain |
| spellingShingle | Zhu YF Kan P Singh G Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain Journal of Pain Research electrophysiology recording spontaneous pain sensory neuron cancer-induced pain neuropathic pain |
| title | Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain |
| title_full | Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain |
| title_fullStr | Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain |
| title_full_unstemmed | Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain |
| title_short | Differences and Similarities in Spontaneous Activity Between Animal Models of Cancer-Induced Pain and Neuropathic Pain |
| title_sort | differences and similarities in spontaneous activity between animal models of cancer induced pain and neuropathic pain |
| topic | electrophysiology recording spontaneous pain sensory neuron cancer-induced pain neuropathic pain |
| url | https://www.dovepress.com/differences-and-similarities-in-spontaneous-activity-between-animal-mo-peer-reviewed-fulltext-article-JPR |
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