Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle
ABSTRACT: Suboptimal LH surges can lead to delayed or absent ovulation during reproductive management programs in cattle. Several signaling pathways may enhance the LH signal, potentially including growth factors. In the present study, we determined whether Fibroblast growth factor 18 (FGF18), which...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
2023-10-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000500701&tlng=en |
| _version_ | 1827778359005806592 |
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| author | Dalila do Nascimento Reis Daniele Missio Mariani Farias Fiorenza Alfredo Quites Antoniazzi Christopher Price Paulo Bayard Dias Gonçalves Valério Marques Portela |
| author_facet | Dalila do Nascimento Reis Daniele Missio Mariani Farias Fiorenza Alfredo Quites Antoniazzi Christopher Price Paulo Bayard Dias Gonçalves Valério Marques Portela |
| author_sort | Dalila do Nascimento Reis |
| collection | DOAJ |
| description | ABSTRACT: Suboptimal LH surges can lead to delayed or absent ovulation during reproductive management programs in cattle. Several signaling pathways may enhance the LH signal, potentially including growth factors. In the present study, we determined whether Fibroblast growth factor 18 (FGF18), which mimics some actions of the LH surge on granulosa cells, may potentiate the action of a suboptimal dose of LH using an established bovine granulosa cell (GC) culture system. Addition of recombinant FGF18 increased the concentration of progesterone (P4) in the culture medium. A combination of LH and FGF18 in the culture medium increased the abundance of mRNA encoding EREG, EGR1 and EGR3, proteins that are early modulators of the LH-induced pre-ovulatory cascade. This study demonstrated that FGF18 may be a co-factor in the regulation of the pre-ovulatory cascade in bovine ovarian granulosa cells. |
| first_indexed | 2024-03-11T14:32:02Z |
| format | Article |
| id | doaj.art-2a97acc04ca54d2f8c64696f1acccfc4 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-03-11T14:32:02Z |
| publishDate | 2023-10-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-2a97acc04ca54d2f8c64696f1acccfc42023-10-31T07:43:48ZengUniversidade Federal de Santa MariaCiência Rural1678-45962023-10-0154510.1590/0103-8478cr20220215Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattleDalila do Nascimento Reishttps://orcid.org/0000-0001-7680-5153Daniele Missiohttps://orcid.org/0000-0003-3524-1894Mariani Farias Fiorenzahttps://orcid.org/0000-0001-8202-0678Alfredo Quites Antoniazzihttps://orcid.org/0000-0003-4866-5944Christopher Pricehttps://orcid.org/0000-0002-6727-8280Paulo Bayard Dias Gonçalveshttps://orcid.org/0000-0002-0671-1466Valério Marques Portelahttps://orcid.org/0000-0003-2109-1346ABSTRACT: Suboptimal LH surges can lead to delayed or absent ovulation during reproductive management programs in cattle. Several signaling pathways may enhance the LH signal, potentially including growth factors. In the present study, we determined whether Fibroblast growth factor 18 (FGF18), which mimics some actions of the LH surge on granulosa cells, may potentiate the action of a suboptimal dose of LH using an established bovine granulosa cell (GC) culture system. Addition of recombinant FGF18 increased the concentration of progesterone (P4) in the culture medium. A combination of LH and FGF18 in the culture medium increased the abundance of mRNA encoding EREG, EGR1 and EGR3, proteins that are early modulators of the LH-induced pre-ovulatory cascade. This study demonstrated that FGF18 may be a co-factor in the regulation of the pre-ovulatory cascade in bovine ovarian granulosa cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000500701&tlng=enFGF18ovulationgranulosa cells |
| spellingShingle | Dalila do Nascimento Reis Daniele Missio Mariani Farias Fiorenza Alfredo Quites Antoniazzi Christopher Price Paulo Bayard Dias Gonçalves Valério Marques Portela Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle Ciência Rural FGF18 ovulation granulosa cells |
| title | Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle |
| title_full | Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle |
| title_fullStr | Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle |
| title_full_unstemmed | Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle |
| title_short | Role of fibroblast growth factor 18 in regulating the cascade of pre-ovulatory events in cattle |
| title_sort | role of fibroblast growth factor 18 in regulating the cascade of pre ovulatory events in cattle |
| topic | FGF18 ovulation granulosa cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000500701&tlng=en |
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