Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of app...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2013-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631&lng=en&tlng=en |
| _version_ | 1797713336503107584 |
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| author | Ligia Cristina Kalb Souza Rosana Elisa Gonçalves Gonçalves Pinho Carla Vanessa de Paula Lima Stênio Perdigão Fragoso Maurilio José Soares |
| author_facet | Ligia Cristina Kalb Souza Rosana Elisa Gonçalves Gonçalves Pinho Carla Vanessa de Paula Lima Stênio Perdigão Fragoso Maurilio José Soares |
| author_sort | Ligia Cristina Kalb Souza |
| collection | DOAJ |
| description | Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids. |
| first_indexed | 2024-03-12T07:34:53Z |
| format | Article |
| id | doaj.art-2a9ae724d9a241d1a1e219b91c8c6319 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:34:53Z |
| publishDate | 2013-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-2a9ae724d9a241d1a1e219b91c8c63192023-09-02T21:33:01ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602013-08-01108563163610.1590/0074-0276108052013015S0074-02762013000500631Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)Ligia Cristina Kalb SouzaRosana Elisa Gonçalves Gonçalves PinhoCarla Vanessa de Paula LimaStênio Perdigão FragosoMaurilio José SoaresHeteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631&lng=en&tlng=enactinconfocal microscopytrypanosomatidsTrypanosoma cruzi |
| spellingShingle | Ligia Cristina Kalb Souza Rosana Elisa Gonçalves Gonçalves Pinho Carla Vanessa de Paula Lima Stênio Perdigão Fragoso Maurilio José Soares Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea) Memorias do Instituto Oswaldo Cruz actin confocal microscopy trypanosomatids Trypanosoma cruzi |
| title | Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea) |
| title_full | Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea) |
| title_fullStr | Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea) |
| title_full_unstemmed | Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea) |
| title_short | Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea) |
| title_sort | actin expression in trypanosomatids euglenozoa kinetoplastea |
| topic | actin confocal microscopy trypanosomatids Trypanosoma cruzi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631&lng=en&tlng=en |
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