Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies

Camelina oil has a high sterol concentration and is rather expensive compared to other vegetable oils. Because of its higher price, it is often adulterated by the addition of other, cheaper oils. This study was performed to validate a method for sterol determination in camelina oil, enabling the det...

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Main Authors: Zala Kolenc, Tanja Potočnik, Urban Bren, Iztok Jože Košir
Format: Article
Language:English
Published: Slovenian Chemical Society 2020-12-01
Series:Acta Chimica Slovenica
Subjects:
Online Access:https://journals.matheo.si/index.php/ACSi/article/view/6059
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author Zala Kolenc
Tanja Potočnik
Urban Bren
Iztok Jože Košir
author_facet Zala Kolenc
Tanja Potočnik
Urban Bren
Iztok Jože Košir
author_sort Zala Kolenc
collection DOAJ
description Camelina oil has a high sterol concentration and is rather expensive compared to other vegetable oils. Because of its higher price, it is often adulterated by the addition of other, cheaper oils. This study was performed to validate a method for sterol determination in camelina oil, enabling the detection of camelina oil adulteration. Sterol levels in camelina oil samples were determined by gas chromatography after saponification and solid phase extraction. The method was validated, and the results proved that the chosen method is specific and selective, repeatable and accurate. The quantitatively assessed average contents of sterols in camelina oil samples of Slovenian origin were 21.4 mg 100 g-1 for brassicasterol, 153.6 mg 100 g-1 for campesterol, 3.9 mg 100 g-1 for stigmasterol, and 447.0 mg 100 g-1 for b-sitosterol. Results of camelina oil authenticity studies regarding botanical origin, performed by Principal Component Analysis (PCA) and Regularized Discriminant Analysis (RDA) enabled us to differentiate 100 % camelina oils from camelina oils adulterated with 10 %–40 % added sunflower, rapeseed or soya oil.
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spelling doaj.art-2a9b2e8213f941969f02989c38431fe32022-12-21T19:02:53ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552020-12-016741163117110.17344/acsi.2020.6059896Determination of Camelina Oil Sterol Composition and Its Application for Authenticity StudiesZala Kolenc0Tanja Potočnik1Urban Bren2Iztok Jože Košir3University of Maribor, Faculty of Chemistry and Chemical EngineeringFrutaromUniversity of Maribor, Faculty of Chemistry and Chemical EngineeringSlovenian Institute of Hop Research and BrewingCamelina oil has a high sterol concentration and is rather expensive compared to other vegetable oils. Because of its higher price, it is often adulterated by the addition of other, cheaper oils. This study was performed to validate a method for sterol determination in camelina oil, enabling the detection of camelina oil adulteration. Sterol levels in camelina oil samples were determined by gas chromatography after saponification and solid phase extraction. The method was validated, and the results proved that the chosen method is specific and selective, repeatable and accurate. The quantitatively assessed average contents of sterols in camelina oil samples of Slovenian origin were 21.4 mg 100 g-1 for brassicasterol, 153.6 mg 100 g-1 for campesterol, 3.9 mg 100 g-1 for stigmasterol, and 447.0 mg 100 g-1 for b-sitosterol. Results of camelina oil authenticity studies regarding botanical origin, performed by Principal Component Analysis (PCA) and Regularized Discriminant Analysis (RDA) enabled us to differentiate 100 % camelina oils from camelina oils adulterated with 10 %–40 % added sunflower, rapeseed or soya oil.https://journals.matheo.si/index.php/ACSi/article/view/6059camelina sativasterolsauthenticitybotanical originchemometrics
spellingShingle Zala Kolenc
Tanja Potočnik
Urban Bren
Iztok Jože Košir
Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies
Acta Chimica Slovenica
camelina sativa
sterols
authenticity
botanical origin
chemometrics
title Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies
title_full Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies
title_fullStr Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies
title_full_unstemmed Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies
title_short Determination of Camelina Oil Sterol Composition and Its Application for Authenticity Studies
title_sort determination of camelina oil sterol composition and its application for authenticity studies
topic camelina sativa
sterols
authenticity
botanical origin
chemometrics
url https://journals.matheo.si/index.php/ACSi/article/view/6059
work_keys_str_mv AT zalakolenc determinationofcamelinaoilsterolcompositionanditsapplicationforauthenticitystudies
AT tanjapotocnik determinationofcamelinaoilsterolcompositionanditsapplicationforauthenticitystudies
AT urbanbren determinationofcamelinaoilsterolcompositionanditsapplicationforauthenticitystudies
AT iztokjozekosir determinationofcamelinaoilsterolcompositionanditsapplicationforauthenticitystudies