Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization
SNAREs (soluble N-ethylmaleimide-sensitive-factor attachment protein receptors) are engines for almost all of the membrane fusion and exocytosis events in organism cells. In this study, we identified 84 <i>SNARE</i> genes from banana (<i>Musa acuminata</i>). Gene expression a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/8/1599 |
| _version_ | 1797603789438451712 |
|---|---|
| author | Bin Wang Yanbing Xu Shiyao Xu Huan Wu Pengyan Qu Zheng Tong Peitao Lü Chunzhen Cheng |
| author_facet | Bin Wang Yanbing Xu Shiyao Xu Huan Wu Pengyan Qu Zheng Tong Peitao Lü Chunzhen Cheng |
| author_sort | Bin Wang |
| collection | DOAJ |
| description | SNAREs (soluble N-ethylmaleimide-sensitive-factor attachment protein receptors) are engines for almost all of the membrane fusion and exocytosis events in organism cells. In this study, we identified 84 <i>SNARE</i> genes from banana (<i>Musa acuminata</i>). Gene expression analysis revealed that the expression of <i>MaSNARE</i>s varied a lot in different banana organs. By analyzing their expression patterns under low temperature (4 °C), high temperature (45 °C), mutualistic fungus (<i>Serendipita indica</i>, Si) and fungal pathogen (<i>Fusarium oxysporum</i> f. sp. <i>Cubense</i> Tropical Race 4, <i>Foc</i>TR4) treatments, many <i>MaSNAREs</i> were found to be stress responsive. For example, <i>MaBET1d</i> was up-regulate by both low and high temperature stresses; <i>MaNPSN11a</i> was up-regulated by low temperature but down-regulated by high temperature; and <i>Foc</i>TR4 treatment up-regulated the expression of <i>MaSYP121</i> but down-regulated <i>MaVAMP72a</i> and <i>MaSNAP33a</i>. Notably, the upregulation or downregulation effects of <i>Foc</i>TR4 on the expression of some <i>MaSNAREs</i> could be alleviated by priorly colonized Si, suggesting that they play roles in the Si-enhanced banana wilt resistance. <i>Foc</i> resistance assays were performed in tobacco leaves transiently overexpressing <i>MaSYP121</i>, <i>MaVAMP72a</i> and <i>MaSNAP33a</i>. Results showed that transient overexpression of <i>MaSYP121</i> and <i>MaSNPA33a</i> suppressed the penetration and spread of both <i>Foc</i>1 (<i>Foc</i> Race 1) and <i>Foc</i>TR4 in tobacco leaves, suggesting that they play positive roles in resisting <i>Foc</i> infection. However, the transient overexpression of <i>MaVAMP72a</i> facilitated <i>Foc</i> infection. Our study can provide a basis for understanding the roles of <i>MaSNAREs</i> in the banana responses to temperature stress and mutualistic and pathogenic fungal colonization. |
| first_indexed | 2024-03-11T04:37:04Z |
| format | Article |
| id | doaj.art-2a9e879e79404c519b07a44c70889739 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:37:04Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-2a9e879e79404c519b07a44c708897392023-11-17T20:58:58ZengMDPI AGPlants2223-77472023-04-01128159910.3390/plants12081599Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal ColonizationBin Wang0Yanbing Xu1Shiyao Xu2Huan Wu3Pengyan Qu4Zheng Tong5Peitao Lü6Chunzhen Cheng7College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSNAREs (soluble N-ethylmaleimide-sensitive-factor attachment protein receptors) are engines for almost all of the membrane fusion and exocytosis events in organism cells. In this study, we identified 84 <i>SNARE</i> genes from banana (<i>Musa acuminata</i>). Gene expression analysis revealed that the expression of <i>MaSNARE</i>s varied a lot in different banana organs. By analyzing their expression patterns under low temperature (4 °C), high temperature (45 °C), mutualistic fungus (<i>Serendipita indica</i>, Si) and fungal pathogen (<i>Fusarium oxysporum</i> f. sp. <i>Cubense</i> Tropical Race 4, <i>Foc</i>TR4) treatments, many <i>MaSNAREs</i> were found to be stress responsive. For example, <i>MaBET1d</i> was up-regulate by both low and high temperature stresses; <i>MaNPSN11a</i> was up-regulated by low temperature but down-regulated by high temperature; and <i>Foc</i>TR4 treatment up-regulated the expression of <i>MaSYP121</i> but down-regulated <i>MaVAMP72a</i> and <i>MaSNAP33a</i>. Notably, the upregulation or downregulation effects of <i>Foc</i>TR4 on the expression of some <i>MaSNAREs</i> could be alleviated by priorly colonized Si, suggesting that they play roles in the Si-enhanced banana wilt resistance. <i>Foc</i> resistance assays were performed in tobacco leaves transiently overexpressing <i>MaSYP121</i>, <i>MaVAMP72a</i> and <i>MaSNAP33a</i>. Results showed that transient overexpression of <i>MaSYP121</i> and <i>MaSNPA33a</i> suppressed the penetration and spread of both <i>Foc</i>1 (<i>Foc</i> Race 1) and <i>Foc</i>TR4 in tobacco leaves, suggesting that they play positive roles in resisting <i>Foc</i> infection. However, the transient overexpression of <i>MaVAMP72a</i> facilitated <i>Foc</i> infection. Our study can provide a basis for understanding the roles of <i>MaSNAREs</i> in the banana responses to temperature stress and mutualistic and pathogenic fungal colonization.https://www.mdpi.com/2223-7747/12/8/1599bananaSNAREtemperature stress<i>Serendipita indica</i><i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| spellingShingle | Bin Wang Yanbing Xu Shiyao Xu Huan Wu Pengyan Qu Zheng Tong Peitao Lü Chunzhen Cheng Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization Plants banana SNARE temperature stress <i>Serendipita indica</i> <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title | Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization |
| title_full | Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization |
| title_fullStr | Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization |
| title_full_unstemmed | Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization |
| title_short | Characterization of Banana <i>SNARE</i> Genes and Their Expression Analysis under Temperature Stress and Mutualistic and Pathogenic Fungal Colonization |
| title_sort | characterization of banana i snare i genes and their expression analysis under temperature stress and mutualistic and pathogenic fungal colonization |
| topic | banana SNARE temperature stress <i>Serendipita indica</i> <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| url | https://www.mdpi.com/2223-7747/12/8/1599 |
| work_keys_str_mv | AT binwang characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT yanbingxu characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT shiyaoxu characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT huanwu characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT pengyanqu characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT zhengtong characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT peitaolu characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization AT chunzhencheng characterizationofbananaisnareigenesandtheirexpressionanalysisundertemperaturestressandmutualisticandpathogenicfungalcolonization |