The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes
<p>Abstract</p> <p>Background</p> <p>The legume <it>Medicago truncatula</it> has emerged as a model plant for the molecular and genetic dissection of various plant processes involved in rhizobial, mycorrhizal and pathogenic plant-microbe interactions. Aiming...
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| Format: | Article |
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BMC
2002-01-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/2/1 |
| _version_ | 1818063349852667904 |
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| author | Ané Jean-Michel Kereszt Attila Journet Etienne-Pascal Ghérardi Michele Thoquet Philippe Prosperi Jean-Marie Huguet Thierry |
| author_facet | Ané Jean-Michel Kereszt Attila Journet Etienne-Pascal Ghérardi Michele Thoquet Philippe Prosperi Jean-Marie Huguet Thierry |
| author_sort | Ané Jean-Michel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The legume <it>Medicago truncatula</it> has emerged as a model plant for the molecular and genetic dissection of various plant processes involved in rhizobial, mycorrhizal and pathogenic plant-microbe interactions. Aiming to develop essential tools for such genetic approaches, we have established the first genetic map of this species. Two parental homozygous lines were selected from the cultivar Jemalong and from the Algerian natural population (DZA315) on the basis of their molecular and phenotypic polymorphism.</p> <p>Results</p> <p>An F2 segregating population of 124 individuals between these two lines was obtained using an efficient manual crossing technique established for <it>M. truncatula</it> and was used to construct a genetic map. This map spans 1225 cM (average 470 kb/cM) and comprises 289 markers including RAPD, AFLP, known genes and isoenzymes arranged in 8 linkage groups (2n = 16). Markers are uniformly distributed throughout the map and segregation distortion is limited to only 3 linkage groups. By mapping a number of common markers, the eight linkage groups are shown to be homologous to those of diploid alfalfa (<it>M. sativa</it>), implying a good level of macrosynteny between the two genomes. Using this <it>M. truncatula</it> map and the derived F3 populations, we were able to map the <it>Mtsym6</it> symbiotic gene on linkage group 8 and the <it>SPC</it> gene, responsible for the direction of pod coiling, on linkage group 7.</p> <p>Conclusions</p> <p>These results demonstrate that <it>Medicago truncatula</it> is amenable to diploid genetic analysis and they open the way to map-based cloning of symbiotic or other agronomically-important genes using this model plant.</p> |
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| id | doaj.art-2aa1fd31221a402ea8e53005e524dea9 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-10T14:18:42Z |
| publishDate | 2002-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-2aa1fd31221a402ea8e53005e524dea92022-12-22T01:45:16ZengBMCBMC Plant Biology1471-22292002-01-0121110.1186/1471-2229-2-1The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genesAné Jean-MichelKereszt AttilaJournet Etienne-PascalGhérardi MicheleThoquet PhilippeProsperi Jean-MarieHuguet Thierry<p>Abstract</p> <p>Background</p> <p>The legume <it>Medicago truncatula</it> has emerged as a model plant for the molecular and genetic dissection of various plant processes involved in rhizobial, mycorrhizal and pathogenic plant-microbe interactions. Aiming to develop essential tools for such genetic approaches, we have established the first genetic map of this species. Two parental homozygous lines were selected from the cultivar Jemalong and from the Algerian natural population (DZA315) on the basis of their molecular and phenotypic polymorphism.</p> <p>Results</p> <p>An F2 segregating population of 124 individuals between these two lines was obtained using an efficient manual crossing technique established for <it>M. truncatula</it> and was used to construct a genetic map. This map spans 1225 cM (average 470 kb/cM) and comprises 289 markers including RAPD, AFLP, known genes and isoenzymes arranged in 8 linkage groups (2n = 16). Markers are uniformly distributed throughout the map and segregation distortion is limited to only 3 linkage groups. By mapping a number of common markers, the eight linkage groups are shown to be homologous to those of diploid alfalfa (<it>M. sativa</it>), implying a good level of macrosynteny between the two genomes. Using this <it>M. truncatula</it> map and the derived F3 populations, we were able to map the <it>Mtsym6</it> symbiotic gene on linkage group 8 and the <it>SPC</it> gene, responsible for the direction of pod coiling, on linkage group 7.</p> <p>Conclusions</p> <p>These results demonstrate that <it>Medicago truncatula</it> is amenable to diploid genetic analysis and they open the way to map-based cloning of symbiotic or other agronomically-important genes using this model plant.</p>http://www.biomedcentral.com/1471-2229/2/1 |
| spellingShingle | Ané Jean-Michel Kereszt Attila Journet Etienne-Pascal Ghérardi Michele Thoquet Philippe Prosperi Jean-Marie Huguet Thierry The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes BMC Plant Biology |
| title | The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes |
| title_full | The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes |
| title_fullStr | The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes |
| title_full_unstemmed | The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes |
| title_short | The molecular genetic linkage map of the model legume <it>Medicago truncatula</it>: an essential tool for comparative legume genomics and the isolation of agronomically important genes |
| title_sort | molecular genetic linkage map of the model legume it medicago truncatula it an essential tool for comparative legume genomics and the isolation of agronomically important genes |
| url | http://www.biomedcentral.com/1471-2229/2/1 |
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