Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i>
The necrotrophic fungus <i>Botrytis cinerea</i>, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopa...
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MDPI AG
2020-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/7/1043 |
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| author | Gulab Chand Arya Dhruv Aditya Srivastava Eswari P. J. Pandaranayaka Ekaterina Manasherova Dov Bernard Prusky Yigal Elad Omer Frenkel Hay Dvir Arye Harel |
| author_facet | Gulab Chand Arya Dhruv Aditya Srivastava Eswari P. J. Pandaranayaka Ekaterina Manasherova Dov Bernard Prusky Yigal Elad Omer Frenkel Hay Dvir Arye Harel |
| author_sort | Gulab Chand Arya |
| collection | DOAJ |
| description | The necrotrophic fungus <i>Botrytis cinerea</i>, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopathogenic fungi have demonstrated the role of membrane-bound and secreted CFEM-containing proteins in different aspects of fungal virulence. However, non-G protein-coupled receptor (non-GPCR) membrane CFEM proteins have not been studied yet in phytopathogenic fungi. In the present study, we have identified a non-GPCR membrane-bound CFEM-containing protein, Bcin07g03260, in the <i>B. cinerea</i> genome, and generated deletion mutants, ΔCFEM-<i>Bcin07g03260</i>, to study its potential role in physiology and virulence. Three independent ΔCFEM-<i>Bcin07g03260</i> mutants showed significantly reduced progression of a necrotic lesion on tomato (<i>Solanum lycopersicum</i>) leaves. Further analysis of the mutants revealed significant reduction (approximately 20–30%) in conidial germination and consequent germ tube elongation compared with the WT. Our data complements a previous study of secreted Δ<i>CFEM1</i> mutants of <i>B. cinerea</i> that showed reduced progression of necrotic lesions on leaves, without effect on germination. Considering various functions identified for CFEM proteins in fungal virulence, our work illustrates a potential new role for a non-GPCR membrane CFEM in pathogenic fungi to control virulence in the fungus <i>B. cinerea</i>. |
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| last_indexed | 2024-03-10T18:29:23Z |
| publishDate | 2020-07-01 |
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| series | Microorganisms |
| spelling | doaj.art-2aa3f8d71b6d45c4b5ef66cfd2524d552023-11-20T06:42:52ZengMDPI AGMicroorganisms2076-26072020-07-0187104310.3390/microorganisms8071043Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i>Gulab Chand Arya0Dhruv Aditya Srivastava1Eswari P. J. Pandaranayaka2Ekaterina Manasherova3Dov Bernard Prusky4Yigal Elad5Omer Frenkel6Hay Dvir7Arye Harel8Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Postharvest Science, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion7505101, IsraelDepartment of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Ruminant Science, Institute of Animal Science, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelDepartment of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, IsraelThe necrotrophic fungus <i>Botrytis cinerea</i>, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopathogenic fungi have demonstrated the role of membrane-bound and secreted CFEM-containing proteins in different aspects of fungal virulence. However, non-G protein-coupled receptor (non-GPCR) membrane CFEM proteins have not been studied yet in phytopathogenic fungi. In the present study, we have identified a non-GPCR membrane-bound CFEM-containing protein, Bcin07g03260, in the <i>B. cinerea</i> genome, and generated deletion mutants, ΔCFEM-<i>Bcin07g03260</i>, to study its potential role in physiology and virulence. Three independent ΔCFEM-<i>Bcin07g03260</i> mutants showed significantly reduced progression of a necrotic lesion on tomato (<i>Solanum lycopersicum</i>) leaves. Further analysis of the mutants revealed significant reduction (approximately 20–30%) in conidial germination and consequent germ tube elongation compared with the WT. Our data complements a previous study of secreted Δ<i>CFEM1</i> mutants of <i>B. cinerea</i> that showed reduced progression of necrotic lesions on leaves, without effect on germination. Considering various functions identified for CFEM proteins in fungal virulence, our work illustrates a potential new role for a non-GPCR membrane CFEM in pathogenic fungi to control virulence in the fungus <i>B. cinerea</i>.https://www.mdpi.com/2076-2607/8/7/1043<i>Botrytis cinerea</i>virulenceCFEMgerminationpathogenicity |
| spellingShingle | Gulab Chand Arya Dhruv Aditya Srivastava Eswari P. J. Pandaranayaka Ekaterina Manasherova Dov Bernard Prusky Yigal Elad Omer Frenkel Hay Dvir Arye Harel Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i> Microorganisms <i>Botrytis cinerea</i> virulence CFEM germination pathogenicity |
| title | Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i> |
| title_full | Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i> |
| title_fullStr | Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i> |
| title_full_unstemmed | Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i> |
| title_short | Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of <i>Botrytis cinerea</i> |
| title_sort | characterization of the role of a non gpcr membrane bound cfem protein in the pathogenicity and germination of i botrytis cinerea i |
| topic | <i>Botrytis cinerea</i> virulence CFEM germination pathogenicity |
| url | https://www.mdpi.com/2076-2607/8/7/1043 |
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