Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus
SIVmac239 infection of macaques is a favored model of human HIV infection, but antibody-mediated protection for SIVmac239 is insufficiently understood. Here, Zhao and Berndsen et al isolated nAbs and confirmed protection against SIVmac239 infection in passive transfer studies in macaques. The nAb wa...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32783-2 |
| _version_ | 1811273658992164864 |
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| author | Fangzhu Zhao Zachary T. Berndsen Nuria Pedreño-Lopez Alison Burns Joel D. Allen Shawn Barman Wen-Hsin Lee Srirupa Chakraborty Sandrasegaram Gnanakaran Leigh M. Sewall Gabriel Ozorowski Oliver Limbo Ge Song Peter Yong Sean Callaghan Jessica Coppola Kim L. Weisgrau Jeffrey D. Lifson Rebecca Nedellec Thomas B. Voigt Fernanda Laurino Johan Louw Brandon C. Rosen Michael Ricciardi Max Crispin Ronald C. Desrosiers Eva G. Rakasz David I. Watkins Raiees Andrabi Andrew B. Ward Dennis R. Burton Devin Sok |
| author_facet | Fangzhu Zhao Zachary T. Berndsen Nuria Pedreño-Lopez Alison Burns Joel D. Allen Shawn Barman Wen-Hsin Lee Srirupa Chakraborty Sandrasegaram Gnanakaran Leigh M. Sewall Gabriel Ozorowski Oliver Limbo Ge Song Peter Yong Sean Callaghan Jessica Coppola Kim L. Weisgrau Jeffrey D. Lifson Rebecca Nedellec Thomas B. Voigt Fernanda Laurino Johan Louw Brandon C. Rosen Michael Ricciardi Max Crispin Ronald C. Desrosiers Eva G. Rakasz David I. Watkins Raiees Andrabi Andrew B. Ward Dennis R. Burton Devin Sok |
| author_sort | Fangzhu Zhao |
| collection | DOAJ |
| description | SIVmac239 infection of macaques is a favored model of human HIV infection, but antibody-mediated protection for SIVmac239 is insufficiently understood. Here, Zhao and Berndsen et al isolated nAbs and confirmed protection against SIVmac239 infection in passive transfer studies in macaques. The nAb was used to provide the first high-resolution structure of a rhesus SIV trimer by CryoEM. Analysis of the glycosylation pattern of this SIV trimer suggests a denser glycan shield on Env for rhesus SIV compared to chimpanzee SIV or HIV-1, which partially explains the poor nAb response of rhesus macaques to SIVmac239 infection. |
| first_indexed | 2024-04-12T23:04:26Z |
| format | Article |
| id | doaj.art-2aa7798e6f944e178232a7a70fe98e0c |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T23:04:26Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-2aa7798e6f944e178232a7a70fe98e0c2022-12-22T03:12:58ZengNature PortfolioNature Communications2041-17232022-09-0113111610.1038/s41467-022-32783-2Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virusFangzhu Zhao0Zachary T. Berndsen1Nuria Pedreño-Lopez2Alison Burns3Joel D. Allen4Shawn Barman5Wen-Hsin Lee6Srirupa Chakraborty7Sandrasegaram Gnanakaran8Leigh M. Sewall9Gabriel Ozorowski10Oliver Limbo11Ge Song12Peter Yong13Sean Callaghan14Jessica Coppola15Kim L. Weisgrau16Jeffrey D. Lifson17Rebecca Nedellec18Thomas B. Voigt19Fernanda Laurino20Johan Louw21Brandon C. Rosen22Michael Ricciardi23Max Crispin24Ronald C. Desrosiers25Eva G. Rakasz26David I. Watkins27Raiees Andrabi28Andrew B. Ward29Dennis R. Burton30Devin Sok31Department of Immunology and Microbiology, The Scripps Research InstituteIAVI Neutralizing Antibody Center, The Scripps Research InstituteDepartment of Pathology, George Washington UniversityDepartment of Immunology and Microbiology, The Scripps Research InstituteSchool of Biological Sciences, University of SouthamptonDepartment of Immunology and Microbiology, The Scripps Research InstituteIAVI Neutralizing Antibody Center, The Scripps Research InstituteTheoretical Biology and Biophysics Group, Los Alamos National LaboratoryTheoretical Biology and Biophysics Group, Los Alamos National LaboratoryIAVI Neutralizing Antibody Center, The Scripps Research InstituteIAVI Neutralizing Antibody Center, The Scripps Research InstituteIAVI Neutralizing Antibody Center, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteWisconsin National Primate Research Center, University of Wisconsin-MadisonAIDS and Cancer Virus Program, Frederick National Laboratory for Cancer ResearchDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Pathology, George Washington UniversityDepartment of Pathology, George Washington UniversityDepartment of Pathology, George Washington UniversityDepartment of Pathology, George Washington UniversityDepartment of Pathology, George Washington UniversitySchool of Biological Sciences, University of SouthamptonDepartment of Pathology, Miller School of Medicine, University of MiamiWisconsin National Primate Research Center, University of Wisconsin-MadisonDepartment of Pathology, George Washington UniversityDepartment of Immunology and Microbiology, The Scripps Research InstituteIAVI Neutralizing Antibody Center, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteSIVmac239 infection of macaques is a favored model of human HIV infection, but antibody-mediated protection for SIVmac239 is insufficiently understood. Here, Zhao and Berndsen et al isolated nAbs and confirmed protection against SIVmac239 infection in passive transfer studies in macaques. The nAb was used to provide the first high-resolution structure of a rhesus SIV trimer by CryoEM. Analysis of the glycosylation pattern of this SIV trimer suggests a denser glycan shield on Env for rhesus SIV compared to chimpanzee SIV or HIV-1, which partially explains the poor nAb response of rhesus macaques to SIVmac239 infection.https://doi.org/10.1038/s41467-022-32783-2 |
| spellingShingle | Fangzhu Zhao Zachary T. Berndsen Nuria Pedreño-Lopez Alison Burns Joel D. Allen Shawn Barman Wen-Hsin Lee Srirupa Chakraborty Sandrasegaram Gnanakaran Leigh M. Sewall Gabriel Ozorowski Oliver Limbo Ge Song Peter Yong Sean Callaghan Jessica Coppola Kim L. Weisgrau Jeffrey D. Lifson Rebecca Nedellec Thomas B. Voigt Fernanda Laurino Johan Louw Brandon C. Rosen Michael Ricciardi Max Crispin Ronald C. Desrosiers Eva G. Rakasz David I. Watkins Raiees Andrabi Andrew B. Ward Dennis R. Burton Devin Sok Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus Nature Communications |
| title | Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus |
| title_full | Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus |
| title_fullStr | Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus |
| title_full_unstemmed | Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus |
| title_short | Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus |
| title_sort | molecular insights into antibody mediated protection against the prototypic simian immunodeficiency virus |
| url | https://doi.org/10.1038/s41467-022-32783-2 |
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