OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice
Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resist...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2022.2094003 |
| _version_ | 1828332052408172544 |
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| author | Rupnaz Kaur Pankaj Kumar Arun Kumar Umesh Preethi Praba Rancy Birdi Rajveer Singh Gurwinder Kaur Jagjeet Singh Lore Kumari Neelam Yogesh Vikal |
| author_facet | Rupnaz Kaur Pankaj Kumar Arun Kumar Umesh Preethi Praba Rancy Birdi Rajveer Singh Gurwinder Kaur Jagjeet Singh Lore Kumari Neelam Yogesh Vikal |
| author_sort | Rupnaz Kaur |
| collection | DOAJ |
| description | Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance. |
| first_indexed | 2024-04-13T21:00:44Z |
| format | Article |
| id | doaj.art-2aa8e4e6cf4b487c90dd9c4cfcaf1334 |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-04-13T21:00:44Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-2aa8e4e6cf4b487c90dd9c4cfcaf13342022-12-22T02:30:09ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532022-12-0117174475510.1080/17429145.2022.2094003OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in riceRupnaz Kaur0Pankaj Kumar1Arun Kumar2Umesh Preethi Praba3Rancy Birdi4Rajveer Singh5Gurwinder Kaur6Jagjeet Singh Lore7Kumari Neelam8Yogesh Vikal9School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, IndiaSheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance.https://www.tandfonline.com/doi/10.1080/17429145.2022.2094003Oryza sativa LOsMATEphylogenyprotein–protein interactionsRNA-seqqRT-PCR |
| spellingShingle | Rupnaz Kaur Pankaj Kumar Arun Kumar Umesh Preethi Praba Rancy Birdi Rajveer Singh Gurwinder Kaur Jagjeet Singh Lore Kumari Neelam Yogesh Vikal OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice Journal of Plant Interactions Oryza sativa L OsMATE phylogeny protein–protein interactions RNA-seq qRT-PCR |
| title | OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice |
| title_full | OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice |
| title_fullStr | OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice |
| title_full_unstemmed | OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice |
| title_short | OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice |
| title_sort | osmate6 gene putatively involved in host defense response toward susceptibility against rhizoctonia solani in rice |
| topic | Oryza sativa L OsMATE phylogeny protein–protein interactions RNA-seq qRT-PCR |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2022.2094003 |
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