The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis
Mitochondria and chloroplasts are important actors in the plant nutritional efficiency. So, it could be expected that a disruption of the coadaptation between nuclear and organellar genomes impact plant response to nutrient stresses. We addressed this issue using two <i>Arabidopsis</i> a...
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MDPI AG
2020-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/5/573 |
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| author | Fabien Chardon Gwendal Cueff Etienne Delannoy Fabien Aubé Aurélia Lornac Magali Bedu Françoise Gilard Stéphanie Pateyron Hélène Rogniaux Audrey Gargaros Hakim Mireau Loïc Rajjou Marie-Laure Martin-Magniette Françoise Budar |
| author_facet | Fabien Chardon Gwendal Cueff Etienne Delannoy Fabien Aubé Aurélia Lornac Magali Bedu Françoise Gilard Stéphanie Pateyron Hélène Rogniaux Audrey Gargaros Hakim Mireau Loïc Rajjou Marie-Laure Martin-Magniette Françoise Budar |
| author_sort | Fabien Chardon |
| collection | DOAJ |
| description | Mitochondria and chloroplasts are important actors in the plant nutritional efficiency. So, it could be expected that a disruption of the coadaptation between nuclear and organellar genomes impact plant response to nutrient stresses. We addressed this issue using two <i>Arabidopsis</i> accessions, namely <i>Ct-1</i> and <i>Jea</i>, and their reciprocal cytolines possessing the nuclear genome from one parent and the organellar genomes of the other one. We measured gene expression, and quantified proteins and metabolites under N starvation and non-limiting conditions. We observed a typical response to N starvation at the phenotype and molecular levels. The phenotypical response to N starvation was similar in the cytolines compared to the parents. However, we observed an effect of the disruption of genomic coadaptation at the molecular levels, distinct from the previously described responses to organellar stresses. Strikingly, genes differentially expressed in cytolines compared to parents were mainly repressed in the cytolines. These genes encoded more mitochondrial and nuclear proteins than randomly expected, while N starvation responsive ones were enriched in genes for chloroplast and nuclear proteins. In cytolines, the non-coadapted cytonuclear genomic combination tends to modulate the response to N starvation observed in the parental lines on various biological processes. |
| first_indexed | 2024-03-10T20:06:16Z |
| format | Article |
| id | doaj.art-2aa9d94c85414131b0c800f509a770ad |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:06:16Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-2aa9d94c85414131b0c800f509a770ad2023-11-19T23:13:19ZengMDPI AGPlants2223-77472020-05-019557310.3390/plants9050573The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in ArabidopsisFabien Chardon0Gwendal Cueff1Etienne Delannoy2Fabien Aubé3Aurélia Lornac4Magali Bedu5Françoise Gilard6Stéphanie Pateyron7Hélène Rogniaux8Audrey Gargaros9Hakim Mireau10Loïc Rajjou11Marie-Laure Martin-Magniette12Françoise Budar13Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, CNRS, INRAE, Univ Evry, 91405 Orsay, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, CNRS, INRAE, Univ Evry, 91405 Orsay, FranceInstitute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, CNRS, INRAE, Univ Evry, 91405 Orsay, FranceINRAE, UR BIA, F-44316 Nantes, FranceINRAE, UR BIA, F-44316 Nantes, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, CNRS, INRAE, Univ Evry, 91405 Orsay, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceMitochondria and chloroplasts are important actors in the plant nutritional efficiency. So, it could be expected that a disruption of the coadaptation between nuclear and organellar genomes impact plant response to nutrient stresses. We addressed this issue using two <i>Arabidopsis</i> accessions, namely <i>Ct-1</i> and <i>Jea</i>, and their reciprocal cytolines possessing the nuclear genome from one parent and the organellar genomes of the other one. We measured gene expression, and quantified proteins and metabolites under N starvation and non-limiting conditions. We observed a typical response to N starvation at the phenotype and molecular levels. The phenotypical response to N starvation was similar in the cytolines compared to the parents. However, we observed an effect of the disruption of genomic coadaptation at the molecular levels, distinct from the previously described responses to organellar stresses. Strikingly, genes differentially expressed in cytolines compared to parents were mainly repressed in the cytolines. These genes encoded more mitochondrial and nuclear proteins than randomly expected, while N starvation responsive ones were enriched in genes for chloroplast and nuclear proteins. In cytolines, the non-coadapted cytonuclear genomic combination tends to modulate the response to N starvation observed in the parental lines on various biological processes.https://www.mdpi.com/2223-7747/9/5/573nutrient stressnatural variationcytonuclear co adaptationtranscriptomeproteomemetabolome |
| spellingShingle | Fabien Chardon Gwendal Cueff Etienne Delannoy Fabien Aubé Aurélia Lornac Magali Bedu Françoise Gilard Stéphanie Pateyron Hélène Rogniaux Audrey Gargaros Hakim Mireau Loïc Rajjou Marie-Laure Martin-Magniette Françoise Budar The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis Plants nutrient stress natural variation cytonuclear co adaptation transcriptome proteome metabolome |
| title | The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis |
| title_full | The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis |
| title_fullStr | The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis |
| title_full_unstemmed | The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis |
| title_short | The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis |
| title_sort | consequences of a disruption in cyto nuclear coadaptation on the molecular response to a nitrate starvation in arabidopsis |
| topic | nutrient stress natural variation cytonuclear co adaptation transcriptome proteome metabolome |
| url | https://www.mdpi.com/2223-7747/9/5/573 |
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