A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers
Abstract An electrochemically based antibody immobilization was used to perform environmentally and clinically relevant immunoassays for stress hormones biomarkers (cortisol and cortisone) using chemiluminescence (CL) detection. To achieve CL detection, the ferrocene tag on the antibodies was first...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-12-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40543-019-0196-y |
| _version_ | 1818929004009226240 |
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| author | Jwan O. Abdulsattar Gillian M. Greenway |
| author_facet | Jwan O. Abdulsattar Gillian M. Greenway |
| author_sort | Jwan O. Abdulsattar |
| collection | DOAJ |
| description | Abstract An electrochemically based antibody immobilization was used to perform environmentally and clinically relevant immunoassays for stress hormones biomarkers (cortisol and cortisone) using chemiluminescence (CL) detection. To achieve CL detection, the ferrocene tag on the antibodies was first oxidised, and this then acted as a catalyst for the luminol and hydrogen peroxide CL reaction. The conditions were optimised and measurements were made with an incubation time of 30 min. Using this approach limits of detection were obtained of 0.47 pg ml−1 and 0.34 pg ml−1 also R 2 0.9912 and 0.9902 for cortisol and cortisone respectively with a linear concentration from 0 to 50 ng ml−1. The method was then applied to Zebrafish whole body and artificial saliva samples. For the Zebrafish sample recoveries of 91.0% and 90.0% were obtained with samples spiked with cortisol and cortisone, for artificial saliva the recoveries were 92.59% and 90.73% respectively. Interference studies showed only minor effects on the measurement of the analyte. A comparison between this procedure and the standard enzyme-linked immunosorbent assay (ELISA) procedure gave approximately the same R 2 values. |
| first_indexed | 2024-12-20T03:37:54Z |
| format | Article |
| id | doaj.art-2aac9555fd694343a3177109aa8c5c91 |
| institution | Directory Open Access Journal |
| issn | 2093-3371 |
| language | English |
| last_indexed | 2024-12-20T03:37:54Z |
| publishDate | 2019-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-2aac9555fd694343a3177109aa8c5c912022-12-21T19:54:50ZengSpringerOpenJournal of Analytical Science and Technology2093-33712019-12-0110111310.1186/s40543-019-0196-yA sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkersJwan O. Abdulsattar0Gillian M. Greenway1Department of Chemistry, College of Science, Mustansiriyah UniversityDepartement of Chemistry and Biochemistry, University of HullAbstract An electrochemically based antibody immobilization was used to perform environmentally and clinically relevant immunoassays for stress hormones biomarkers (cortisol and cortisone) using chemiluminescence (CL) detection. To achieve CL detection, the ferrocene tag on the antibodies was first oxidised, and this then acted as a catalyst for the luminol and hydrogen peroxide CL reaction. The conditions were optimised and measurements were made with an incubation time of 30 min. Using this approach limits of detection were obtained of 0.47 pg ml−1 and 0.34 pg ml−1 also R 2 0.9912 and 0.9902 for cortisol and cortisone respectively with a linear concentration from 0 to 50 ng ml−1. The method was then applied to Zebrafish whole body and artificial saliva samples. For the Zebrafish sample recoveries of 91.0% and 90.0% were obtained with samples spiked with cortisol and cortisone, for artificial saliva the recoveries were 92.59% and 90.73% respectively. Interference studies showed only minor effects on the measurement of the analyte. A comparison between this procedure and the standard enzyme-linked immunosorbent assay (ELISA) procedure gave approximately the same R 2 values.https://doi.org/10.1186/s40543-019-0196-yStress hormonesBiosensorsImmunoassaysChemiluminescence |
| spellingShingle | Jwan O. Abdulsattar Gillian M. Greenway A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers Journal of Analytical Science and Technology Stress hormones Biosensors Immunoassays Chemiluminescence |
| title | A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers |
| title_full | A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers |
| title_fullStr | A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers |
| title_full_unstemmed | A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers |
| title_short | A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers |
| title_sort | sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers |
| topic | Stress hormones Biosensors Immunoassays Chemiluminescence |
| url | https://doi.org/10.1186/s40543-019-0196-y |
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