Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium
In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabli...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Cells |
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| Online Access: | http://www.mdpi.com/2073-4409/8/1/46 |
| _version_ | 1797727477931442176 |
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| author | Tetsuya Muramoto Hoshie Iriki Jun Watanabe Takefumi Kawata |
| author_facet | Tetsuya Muramoto Hoshie Iriki Jun Watanabe Takefumi Kawata |
| author_sort | Tetsuya Muramoto |
| collection | DOAJ |
| description | In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium. |
| first_indexed | 2024-03-12T11:00:18Z |
| format | Article |
| id | doaj.art-2aaf259b101646c48561331be55ee7d0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T11:00:18Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2aaf259b101646c48561331be55ee7d02023-09-02T05:46:41ZengMDPI AGCells2073-44092019-01-01814610.3390/cells8010046cells8010046Recent Advances in CRISPR/Cas9-Mediated Genome Editing in DictyosteliumTetsuya Muramoto0Hoshie Iriki1Jun Watanabe2Takefumi Kawata3Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, JapanDepartment of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, JapanDepartment of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, JapanDepartment of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, JapanIn the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium.http://www.mdpi.com/2073-4409/8/1/46CRISPR/Cas9CRISPR vectorDictyosteliumgenome editingtRNA-based expressionAmoebozoa |
| spellingShingle | Tetsuya Muramoto Hoshie Iriki Jun Watanabe Takefumi Kawata Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium Cells CRISPR/Cas9 CRISPR vector Dictyostelium genome editing tRNA-based expression Amoebozoa |
| title | Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium |
| title_full | Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium |
| title_fullStr | Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium |
| title_full_unstemmed | Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium |
| title_short | Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium |
| title_sort | recent advances in crispr cas9 mediated genome editing in dictyostelium |
| topic | CRISPR/Cas9 CRISPR vector Dictyostelium genome editing tRNA-based expression Amoebozoa |
| url | http://www.mdpi.com/2073-4409/8/1/46 |
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