Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group
Introduction: Polycystic ovary syndrome (PCOS) is one of the most common disorder of women at reproductive age. Many findings indicate that genetic variations in the vitamin D receptor gene may affect PCOS development. This study was performed with aim to evaluate the associations between TaqI polym...
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| Format: | Article |
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Mashhad University of Medical Sciences
2018-10-01
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| Series: | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
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| Online Access: | https://ijogi.mums.ac.ir/article_11976_834649b9690a38216147a0bee5ef4d15.pdf |
| _version_ | 1811223628470026240 |
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| author | Mina Tavakkoli Maryam Ostadsharif Hashem Nayeri |
| author_facet | Mina Tavakkoli Maryam Ostadsharif Hashem Nayeri |
| author_sort | Mina Tavakkoli |
| collection | DOAJ |
| description | Introduction: Polycystic ovary syndrome (PCOS) is one of the most common disorder of women at reproductive age. Many findings indicate that genetic variations in the vitamin D receptor gene may affect PCOS development. This study was performed with aim to evaluate the associations between TaqI polymorphism in the vitamin D receptor gene (VDR) and PCOS, and the association of TaqI polymorphism with gonadotropins and steroid hormones parameters in two groups of patients and control.
Methods: This case-control study was performed on 77 women who had referred to fertility and infertility center of Isfahan during three months of summer 2016. 38 obese women with PCOS were selected as patient group and 39 healthy obese women as control group. Serum levels of LH, FSH, testosterone and progesterone were measured by ELISA method. The TaqI polymorphism was genotyped by using PCR-RFLP (restriction fragment length polymorphism) technique. Data was analyzed by SPSS software (version 22) and independent t-test, Mann-Whitney, Kruskal-Wallis, and Chi-square test. P<0.05 was considered statistically significant.
Results: Based on the results of Chi-square test, no difference was found between T and t alleles and TaqI genotypes in PCOS and control women (p> 0.05). Serum levels of FSH (p = 0.012) and testosterone (p = 0.017) was significantly lower in control group than the patient group. There was no significant difference in serum LH and progesterone between patients and control group (P> 0.05). Also, the level of LH hormone was significantly higher in TT individuals than that of Tt / tt subjects (p = 0.01).
Conclusion: Among the studied population, there was no significant relationship between tt, Tt and TT genotypes with FSH, testosterone and progesterone hormones. Therefore, it can be concluded that TaqI polymorphism was not associated with the risk of polycystic ovary syndrome. |
| first_indexed | 2024-04-12T08:36:02Z |
| format | Article |
| id | doaj.art-2ab090ab372c4045a355fa78cae83063 |
| institution | Directory Open Access Journal |
| issn | 1680-2993 2008-2363 |
| language | fas |
| last_indexed | 2024-04-12T08:36:02Z |
| publishDate | 2018-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| spelling | doaj.art-2ab090ab372c4045a355fa78cae830632022-12-22T03:40:00ZfasMashhad University of Medical SciencesMajallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān1680-29932008-23632018-10-012189410210.22038/ijogi.2018.1197611976Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control groupMina Tavakkoli0Maryam Ostadsharif1Hashem Nayeri2M.Sc. student in Biochemistry, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.Assistant professor, Department of Medical Basic Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran. Assistant professor, Transgenesis Center of Excellence, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.Assistant Professor, Department of Biochemistry, Islamic Azad University, Falavarjan Branch, Isfahan, IranIntroduction: Polycystic ovary syndrome (PCOS) is one of the most common disorder of women at reproductive age. Many findings indicate that genetic variations in the vitamin D receptor gene may affect PCOS development. This study was performed with aim to evaluate the associations between TaqI polymorphism in the vitamin D receptor gene (VDR) and PCOS, and the association of TaqI polymorphism with gonadotropins and steroid hormones parameters in two groups of patients and control. Methods: This case-control study was performed on 77 women who had referred to fertility and infertility center of Isfahan during three months of summer 2016. 38 obese women with PCOS were selected as patient group and 39 healthy obese women as control group. Serum levels of LH, FSH, testosterone and progesterone were measured by ELISA method. The TaqI polymorphism was genotyped by using PCR-RFLP (restriction fragment length polymorphism) technique. Data was analyzed by SPSS software (version 22) and independent t-test, Mann-Whitney, Kruskal-Wallis, and Chi-square test. P<0.05 was considered statistically significant. Results: Based on the results of Chi-square test, no difference was found between T and t alleles and TaqI genotypes in PCOS and control women (p> 0.05). Serum levels of FSH (p = 0.012) and testosterone (p = 0.017) was significantly lower in control group than the patient group. There was no significant difference in serum LH and progesterone between patients and control group (P> 0.05). Also, the level of LH hormone was significantly higher in TT individuals than that of Tt / tt subjects (p = 0.01). Conclusion: Among the studied population, there was no significant relationship between tt, Tt and TT genotypes with FSH, testosterone and progesterone hormones. Therefore, it can be concluded that TaqI polymorphism was not associated with the risk of polycystic ovary syndrome.https://ijogi.mums.ac.ir/article_11976_834649b9690a38216147a0bee5ef4d15.pdfvitamin d receptorpolycystic ovary syndromesteroid hormonesgonadotropins |
| spellingShingle | Mina Tavakkoli Maryam Ostadsharif Hashem Nayeri Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān vitamin d receptor polycystic ovary syndrome steroid hormones gonadotropins |
| title | Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group |
| title_full | Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group |
| title_fullStr | Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group |
| title_full_unstemmed | Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group |
| title_short | Study of vitamin D receptor gene TaqI polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group |
| title_sort | study of vitamin d receptor gene taqi polymorphism and its association with levels of gonadotropins and steroid hormones between obese women with polycystic ovarian syndrome and obese women in control group |
| topic | vitamin d receptor polycystic ovary syndrome steroid hormones gonadotropins |
| url | https://ijogi.mums.ac.ir/article_11976_834649b9690a38216147a0bee5ef4d15.pdf |
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