Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers
The homeostasis dysfunctions caused by cold stress remain a threat to intestinal health, particularly for young broiler chickens. We hypothesized that adenosine monophosphate-activated protein kinase (AMPK) was involved in the regulation of cold stress on intestinal health. This study aimed to exami...
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| Format: | Article |
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Elsevier
2021-03-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731120301403 |
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| author | H.J. Zhou L.L. Kong L.X. Zhu X.Y. Hu J. Busye Z.G. Song |
| author_facet | H.J. Zhou L.L. Kong L.X. Zhu X.Y. Hu J. Busye Z.G. Song |
| author_sort | H.J. Zhou |
| collection | DOAJ |
| description | The homeostasis dysfunctions caused by cold stress remain a threat to intestinal health, particularly for young broiler chickens. We hypothesized that adenosine monophosphate-activated protein kinase (AMPK) was involved in the regulation of cold stress on intestinal health. This study aimed to examine the effect of cold stress for 72 h on growth performance, serum biochemistry, intestinal barrier molecules, and AMPK in broilers. A total of 144 10-day-old male Arbor Acres broilers were subjected to temperature treatments (control 28 ± 1 °C vs cold stress 16 ± 1 °C) for 72 h. Growth performance was monitored, serum was collected for the analysis of physiological parameters, and jejunal mucosa was sampled for the determination of tight junction (TJ) proteins, heat shock proteins, and AMPK signaling molecules. Results showed that 72 h cold treatment reduced average BW gain and increased the feed conversion ratio of the broilers (P < 0.05). Cold stress for 72 h increased blood endotoxin, aspartate aminotransferase, glucose, and low-density lipoprotein cholesterol levels (P < 0.05). Moreover, 72 h cold treatment up-regulated jejunal Occludin, zonula occludin 1, inducible nitric oxide synthase, heat shock factor 1, and AMPKα1 gene expression (P < 0.05) but had no obvious effect on total AMPK protein expression (P > 0.05). In conclusion, cold stress significantly reduced the growth performance of broiler chickens. The intestinal barrier function might be impaired, and enhanced bacterial translocation might occur. The unregulated gene expression of TJ proteins implied the remodeling of intestinal barrier. The change of AMPK suggested the possible relationship between intestinal energy metabolism and barrier function under cold stress. |
| first_indexed | 2024-12-21T15:23:51Z |
| format | Article |
| id | doaj.art-2ab29adbc83f476ba0b9780c4368d90d |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-21T15:23:51Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-2ab29adbc83f476ba0b9780c4368d90d2022-12-21T18:58:57ZengElsevierAnimal1751-73112021-03-01153100138Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilersH.J. Zhou0L.L. Kong1L.X. Zhu2X.Y. Hu3J. Busye4Z.G. Song5Department of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, ChinaDepartment of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, ChinaDepartment of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, ChinaDivision Animal and Human Health Engineering, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, BelgiumDepartment of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, China; Corresponding author.The homeostasis dysfunctions caused by cold stress remain a threat to intestinal health, particularly for young broiler chickens. We hypothesized that adenosine monophosphate-activated protein kinase (AMPK) was involved in the regulation of cold stress on intestinal health. This study aimed to examine the effect of cold stress for 72 h on growth performance, serum biochemistry, intestinal barrier molecules, and AMPK in broilers. A total of 144 10-day-old male Arbor Acres broilers were subjected to temperature treatments (control 28 ± 1 °C vs cold stress 16 ± 1 °C) for 72 h. Growth performance was monitored, serum was collected for the analysis of physiological parameters, and jejunal mucosa was sampled for the determination of tight junction (TJ) proteins, heat shock proteins, and AMPK signaling molecules. Results showed that 72 h cold treatment reduced average BW gain and increased the feed conversion ratio of the broilers (P < 0.05). Cold stress for 72 h increased blood endotoxin, aspartate aminotransferase, glucose, and low-density lipoprotein cholesterol levels (P < 0.05). Moreover, 72 h cold treatment up-regulated jejunal Occludin, zonula occludin 1, inducible nitric oxide synthase, heat shock factor 1, and AMPKα1 gene expression (P < 0.05) but had no obvious effect on total AMPK protein expression (P > 0.05). In conclusion, cold stress significantly reduced the growth performance of broiler chickens. The intestinal barrier function might be impaired, and enhanced bacterial translocation might occur. The unregulated gene expression of TJ proteins implied the remodeling of intestinal barrier. The change of AMPK suggested the possible relationship between intestinal energy metabolism and barrier function under cold stress.http://www.sciencedirect.com/science/article/pii/S1751731120301403Heat shock factor 1Inducible nitric oxide synthaseIntestinal healthJejunalZonula occludin 1 |
| spellingShingle | H.J. Zhou L.L. Kong L.X. Zhu X.Y. Hu J. Busye Z.G. Song Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers Animal Heat shock factor 1 Inducible nitric oxide synthase Intestinal health Jejunal Zonula occludin 1 |
| title | Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers |
| title_full | Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers |
| title_fullStr | Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers |
| title_full_unstemmed | Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers |
| title_short | Effects of cold stress on growth performance, serum biochemistry, intestinal barrier molecules, and adenosine monophosphate-activated protein kinase in broilers |
| title_sort | effects of cold stress on growth performance serum biochemistry intestinal barrier molecules and adenosine monophosphate activated protein kinase in broilers |
| topic | Heat shock factor 1 Inducible nitric oxide synthase Intestinal health Jejunal Zonula occludin 1 |
| url | http://www.sciencedirect.com/science/article/pii/S1751731120301403 |
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