Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy
Aim To assess the effect of snakehead fish extract administration in angiogenesis focusing on the level of vascular endothelial growth factor (VEGF), nitric oxide (NO) and VEGF receptor 2 (R2) expression is ischemic stroke models. Methods An experimental study was conducted on 5 groups of ischemic...
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| Format: | Article |
| Language: | English |
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Medical Association of Zenica-Doboj Canton
2020-08-01
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| Series: | Medicinski Glasnik |
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| Online Access: | http://ljkzedo.ba/mgpdf/mg33/03_Nasution_1107_A.pdf |
| _version_ | 1819025496909807616 |
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| author | Iskandar Nasution Hasan Sjahrir Syafruddin Ilyas Muhammad Ichwan |
| author_facet | Iskandar Nasution Hasan Sjahrir Syafruddin Ilyas Muhammad Ichwan |
| author_sort | Iskandar Nasution |
| collection | DOAJ |
| description | Aim To assess the effect of snakehead fish extract administration in angiogenesis focusing on the level of vascular endothelial growth factor (VEGF), nitric oxide (NO) and VEGF receptor 2 (R2) expression is ischemic stroke models.
Methods An experimental study was conducted on 5 groups of ischemic stroke rats models: Group K- without carotid artery ligation, Group K+ with artery ligation, Group P1 with artery ligation and administration of 200 mg/day extract, Group P2 with artery ligation and 400 mg/day extract, and Group P3 with artery ligation and 800 mg/day extract. The VEGF expression and NO levels were assessed on day 3.
Results Snakehead fish extract significantly increased VEGF levels along with increasing doses, in which the highest VEGF level was observed in P3 group (361.7±40.2; p<0.001). The NO level also increased along with an increasing dose of snakehead fish extract, in which the highest NO level was found in P3 group (59.43±0.88 μmol/gr; p<0.001). The VEGFR2 expression also increased significantly after snakehead extract administration along with increasing doses (p<0.001) in which administration of 800 mg extract yielded the highest VEGFR2 expression compared with lower doses (17.7 vs. 15.6; p<0.001)
Conclusion Snakehead fish extract administration increased angiogenesis process marked by an increased level of VEGF, NO and VEGFR2 expression in ischemic stroke rat models. |
| first_indexed | 2024-12-21T05:11:37Z |
| format | Article |
| id | doaj.art-2abbe6b6debf44d68a0893ed8aa18513 |
| institution | Directory Open Access Journal |
| issn | 1840-0132 1840-2445 |
| language | English |
| last_indexed | 2024-12-21T05:11:37Z |
| publishDate | 2020-08-01 |
| publisher | Medical Association of Zenica-Doboj Canton |
| record_format | Article |
| series | Medicinski Glasnik |
| spelling | doaj.art-2abbe6b6debf44d68a0893ed8aa185132022-12-21T19:15:02ZengMedical Association of Zenica-Doboj CantonMedicinski Glasnik1840-01321840-24452020-08-0117242042410.17392/1107-20Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapyIskandar Nasution0Hasan Sjahrir1Syafruddin Ilyas2Muhammad Ichwan3Department of Neurology, School of Medicine, Universitas Sumatera Utara, IndonesiaDepartment of Neurology, School of Medicine, Universitas Sumatera Utara, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, IndonesiaDepartment of Pharmacology, School of Medicine; Universitas Sumatera Utara, IndonesiaAim To assess the effect of snakehead fish extract administration in angiogenesis focusing on the level of vascular endothelial growth factor (VEGF), nitric oxide (NO) and VEGF receptor 2 (R2) expression is ischemic stroke models. Methods An experimental study was conducted on 5 groups of ischemic stroke rats models: Group K- without carotid artery ligation, Group K+ with artery ligation, Group P1 with artery ligation and administration of 200 mg/day extract, Group P2 with artery ligation and 400 mg/day extract, and Group P3 with artery ligation and 800 mg/day extract. The VEGF expression and NO levels were assessed on day 3. Results Snakehead fish extract significantly increased VEGF levels along with increasing doses, in which the highest VEGF level was observed in P3 group (361.7±40.2; p<0.001). The NO level also increased along with an increasing dose of snakehead fish extract, in which the highest NO level was found in P3 group (59.43±0.88 μmol/gr; p<0.001). The VEGFR2 expression also increased significantly after snakehead extract administration along with increasing doses (p<0.001) in which administration of 800 mg extract yielded the highest VEGFR2 expression compared with lower doses (17.7 vs. 15.6; p<0.001) Conclusion Snakehead fish extract administration increased angiogenesis process marked by an increased level of VEGF, NO and VEGFR2 expression in ischemic stroke rat models.http://ljkzedo.ba/mgpdf/mg33/03_Nasution_1107_A.pdfargininenitric oxidecerebral ischemia |
| spellingShingle | Iskandar Nasution Hasan Sjahrir Syafruddin Ilyas Muhammad Ichwan Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy Medicinski Glasnik arginine nitric oxide cerebral ischemia |
| title | Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy |
| title_full | Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy |
| title_fullStr | Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy |
| title_full_unstemmed | Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy |
| title_short | Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy |
| title_sort | snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis an insight of stroke therapy |
| topic | arginine nitric oxide cerebral ischemia |
| url | http://ljkzedo.ba/mgpdf/mg33/03_Nasution_1107_A.pdf |
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