An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol
Summary: Conventional competitive enzyme-linked immunosorbent assay (ELISA) to measure the cortisol level in body fluid consumes a large amount of time, owing to complicated operations involved and requirement for precise control of reagent addition. We developed an automatic microfluidic system to ...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223018977 |
| _version_ | 1797647804682731520 |
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| author | Yecan Wang Hiroshi Murakami Toshihiro Kasama Shigenobu Mitsuzawa Satoru Shinkawa Ryo Miyake Madoka Takai |
| author_facet | Yecan Wang Hiroshi Murakami Toshihiro Kasama Shigenobu Mitsuzawa Satoru Shinkawa Ryo Miyake Madoka Takai |
| author_sort | Yecan Wang |
| collection | DOAJ |
| description | Summary: Conventional competitive enzyme-linked immunosorbent assay (ELISA) to measure the cortisol level in body fluid consumes a large amount of time, owing to complicated operations involved and requirement for precise control of reagent addition. We developed an automatic microfluidic system to detect salivary cortisol rapidly, and an electrospun polystyrene (PS) microfiber-based reactor providing considerable binding sites for antibody immobilization, thus resolving the time limitations of competitive ELISA. Cortisol sample, horseradish peroxidase (HRP)-conjugated cortisol, and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate were delivered to the PS reactor from containers in sequence by pumps automatically. The color variation due to oxidized TMB complex reflects the cortisol concentration level measured using an RGB phototransistor. In addition, the entire procedure from sample introduction to obtaining the photocurrent took only 15 min. This system can be implemented to quantify cortisol from 0.37 ng/mL to 30 ng/mL, and the limit of detection was estimated at 0.37 ng/mL. |
| first_indexed | 2024-03-11T15:22:54Z |
| format | Article |
| id | doaj.art-2abcea14b073414eb193bb5b18f71627 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:22:54Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-2abcea14b073414eb193bb5b18f716272023-10-28T05:08:38ZengElsevieriScience2589-00422023-10-012610107820An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisolYecan Wang0Hiroshi Murakami1Toshihiro Kasama2Shigenobu Mitsuzawa3Satoru Shinkawa4Ryo Miyake5Madoka Takai6Graduate School of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo City, Tokyo 113-8654, JapanGraduate School of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo City, Tokyo 113-8654, JapanGraduate School of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo City, Tokyo 113-8654, JapanHonda Motor Co., Ltd, 8-1 Honcho, Wako, Saitama 351-0114, JapanHonda Motor Co., Ltd, 8-1 Honcho, Wako, Saitama 351-0114, JapanGraduate School of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo City, Tokyo 113-8654, Japan; Corresponding authorGraduate School of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo City, Tokyo 113-8654, Japan; Corresponding authorSummary: Conventional competitive enzyme-linked immunosorbent assay (ELISA) to measure the cortisol level in body fluid consumes a large amount of time, owing to complicated operations involved and requirement for precise control of reagent addition. We developed an automatic microfluidic system to detect salivary cortisol rapidly, and an electrospun polystyrene (PS) microfiber-based reactor providing considerable binding sites for antibody immobilization, thus resolving the time limitations of competitive ELISA. Cortisol sample, horseradish peroxidase (HRP)-conjugated cortisol, and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate were delivered to the PS reactor from containers in sequence by pumps automatically. The color variation due to oxidized TMB complex reflects the cortisol concentration level measured using an RGB phototransistor. In addition, the entire procedure from sample introduction to obtaining the photocurrent took only 15 min. This system can be implemented to quantify cortisol from 0.37 ng/mL to 30 ng/mL, and the limit of detection was estimated at 0.37 ng/mL.http://www.sciencedirect.com/science/article/pii/S2589004223018977Diagnostic technique in health technologyAnalytical chemistry applications |
| spellingShingle | Yecan Wang Hiroshi Murakami Toshihiro Kasama Shigenobu Mitsuzawa Satoru Shinkawa Ryo Miyake Madoka Takai An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol iScience Diagnostic technique in health technology Analytical chemistry applications |
| title | An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol |
| title_full | An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol |
| title_fullStr | An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol |
| title_full_unstemmed | An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol |
| title_short | An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol |
| title_sort | automatic immuno microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol |
| topic | Diagnostic technique in health technology Analytical chemistry applications |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223018977 |
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