Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus)
Background and Aim: Gliricidia sepium is a medium-sized leguminous plant found widely in tropical to subtropical areas. It has been used as a medicinal ingredient and in rodenticides by local communities in both Indonesia and the Philippines. This study aimed to investigate the wound healing effects...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2021-03-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.14/March-2021/37.pdf |
| _version_ | 1818740714254630912 |
|---|---|
| author | Aulaani'am Aulanni'am Krismal Marchel Ora Nisa Ain Ariandini Dyah Kinasih Wuragil Fajar Shodiq Permata Wibi Riawan Ma Asuncion Guiang Beltran |
| author_facet | Aulaani'am Aulanni'am Krismal Marchel Ora Nisa Ain Ariandini Dyah Kinasih Wuragil Fajar Shodiq Permata Wibi Riawan Ma Asuncion Guiang Beltran |
| author_sort | Aulaani'am Aulanni'am |
| collection | DOAJ |
| description | Background and Aim: Gliricidia sepium is a medium-sized leguminous plant found widely in tropical to subtropical areas. It has been used as a medicinal ingredient and in rodenticides by local communities in both Indonesia and the Philippines. This study aimed to investigate the wound healing effects of an ointment containing G. sepium leaves on inflammatory cells using a rat model. We also determined its effect on the expression of interleukin (IL) 6 and IL-1β.
Materials and Methods: We used 16 Wistar male rats aged approximately 2 months and weighing 150-200 g. They were divided into four treatment groups (T1, positive control; T2, negative control; T3, wounds treated with G. sepium from Indonesia; and T4, wounds treated with G. sepium from the Philippines), and the ointment therapies were applied to wounds for 3 days. Hematoxylin and eosin staining was performed to examine the inflammatory cells microscopically. IL-1β and IL-6 expression were observed immunohistochemically.
Results: G. sepium leaves significantly (p<0.05) decreased the number of inflammatory cells, and the expression of IL-1β and IL-6 in the group treated with Indonesian G. sepium leaves was higher than that in the group treated with G. sepium leaves from the Philippines. The leaves contain flavonoids, saponins, and tannins, which act as anti-inflammatory agents to enhance the wound healing process.
Conclusion: Our findings suggest that G. sepium leaves from both the Philippines and Indonesia possess wound healing properties. |
| first_indexed | 2024-12-18T01:45:07Z |
| format | Article |
| id | doaj.art-2abdda79b71d4186acd1edae60e7b6e3 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-18T01:45:07Z |
| publishDate | 2021-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-2abdda79b71d4186acd1edae60e7b6e32022-12-21T21:25:12ZengVeterinary WorldVeterinary World0972-89882231-09162021-03-0114382082410.14202/vetworld.2021.820-824Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus)Aulaani'am Aulanni'am0https://orcid.org/0000-0002-8715-4592Krismal Marchel Ora1Nisa Ain Ariandini2Dyah Kinasih Wuragil3https://orcid.org/0000-0003-0817-3865Fajar Shodiq Permata4https://orcid.org/0000-0003-0971-6278Wibi Riawan5Ma Asuncion Guiang Beltran6https://orcid.org/0000-0002-5982-8983Biochemistry Laboratory, Faculty of Sciences, Brawijaya University, Indonesia.Bachelor of Veterinary Student, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Bachelor of Veterinary Student, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Laboratory of Veterinary Biochemistry, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Laboratory of Veterinary Histology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Department of Molecular and Biochemistry, Faculty of Medicine, Brawijaya University, Indonesia.Department of Microbiology and Veterinary Public Health, College of Veterinary Medicine, Tarlac Agricultural University, The Philippines.Background and Aim: Gliricidia sepium is a medium-sized leguminous plant found widely in tropical to subtropical areas. It has been used as a medicinal ingredient and in rodenticides by local communities in both Indonesia and the Philippines. This study aimed to investigate the wound healing effects of an ointment containing G. sepium leaves on inflammatory cells using a rat model. We also determined its effect on the expression of interleukin (IL) 6 and IL-1β. Materials and Methods: We used 16 Wistar male rats aged approximately 2 months and weighing 150-200 g. They were divided into four treatment groups (T1, positive control; T2, negative control; T3, wounds treated with G. sepium from Indonesia; and T4, wounds treated with G. sepium from the Philippines), and the ointment therapies were applied to wounds for 3 days. Hematoxylin and eosin staining was performed to examine the inflammatory cells microscopically. IL-1β and IL-6 expression were observed immunohistochemically. Results: G. sepium leaves significantly (p<0.05) decreased the number of inflammatory cells, and the expression of IL-1β and IL-6 in the group treated with Indonesian G. sepium leaves was higher than that in the group treated with G. sepium leaves from the Philippines. The leaves contain flavonoids, saponins, and tannins, which act as anti-inflammatory agents to enhance the wound healing process. Conclusion: Our findings suggest that G. sepium leaves from both the Philippines and Indonesia possess wound healing properties.http://www.veterinaryworld.org/Vol.14/March-2021/37.pdfflavonoidgliricidia sepium leavesherbal plantssaponintanninwound healing |
| spellingShingle | Aulaani'am Aulanni'am Krismal Marchel Ora Nisa Ain Ariandini Dyah Kinasih Wuragil Fajar Shodiq Permata Wibi Riawan Ma Asuncion Guiang Beltran Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus) Veterinary World flavonoid gliricidia sepium leaves herbal plants saponin tannin wound healing |
| title | Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus) |
| title_full | Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus) |
| title_fullStr | Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus) |
| title_full_unstemmed | Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus) |
| title_short | Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus) |
| title_sort | wound healing properties of gliricidia sepium leaves from indonesia and the philippines in rats rattus norvegicus |
| topic | flavonoid gliricidia sepium leaves herbal plants saponin tannin wound healing |
| url | http://www.veterinaryworld.org/Vol.14/March-2021/37.pdf |
| work_keys_str_mv | AT aulaaniamaulanniam woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus AT krismalmarchelora woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus AT nisaainariandini woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus AT dyahkinasihwuragil woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus AT fajarshodiqpermata woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus AT wibiriawan woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus AT maasuncionguiangbeltran woundhealingpropertiesofgliricidiasepiumleavesfromindonesiaandthephilippinesinratsrattusnorvegicus |