Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease
Chagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174&lng=en&tlng=en |
| _version_ | 1797703165405036544 |
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| author | Martha Elba Gonzalez-Mejia Enrique Torres-Rasgado Leonardo M Porchia Hilda Rosas Salgado José-Luis Totolhua Arturo Ortega Luisa Clara Regina Hernández-Kelly Guadalupe Ruiz-Vivanco Blanca G Báez-Duarte Ricardo Pérez-Fuentes |
| author_facet | Martha Elba Gonzalez-Mejia Enrique Torres-Rasgado Leonardo M Porchia Hilda Rosas Salgado José-Luis Totolhua Arturo Ortega Luisa Clara Regina Hernández-Kelly Guadalupe Ruiz-Vivanco Blanca G Báez-Duarte Ricardo Pérez-Fuentes |
| author_sort | Martha Elba Gonzalez-Mejia |
| collection | DOAJ |
| description | Chagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels. |
| first_indexed | 2024-03-12T05:00:36Z |
| format | Article |
| id | doaj.art-2abf1708409344298d64da51b64d2ad5 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:00:36Z |
| publishDate | 2014-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-2abf1708409344298d64da51b64d2ad52023-09-03T09:11:45ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-04-01109217418110.1590/0074-0276140339S0074-02762014000200174Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas diseaseMartha Elba Gonzalez-MejiaEnrique Torres-RasgadoLeonardo M PorchiaHilda Rosas SalgadoJosé-Luis TotolhuaArturo OrtegaLuisa Clara Regina Hernández-KellyGuadalupe Ruiz-VivancoBlanca G Báez-DuarteRicardo Pérez-FuentesChagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174&lng=en&tlng=enmetallothionein-Iantioxidantsnitric oxideL-NAMEChagas disease |
| spellingShingle | Martha Elba Gonzalez-Mejia Enrique Torres-Rasgado Leonardo M Porchia Hilda Rosas Salgado José-Luis Totolhua Arturo Ortega Luisa Clara Regina Hernández-Kelly Guadalupe Ruiz-Vivanco Blanca G Báez-Duarte Ricardo Pérez-Fuentes Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease Memorias do Instituto Oswaldo Cruz metallothionein-I antioxidants nitric oxide L-NAME Chagas disease |
| title | Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
| title_full | Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
| title_fullStr | Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
| title_full_unstemmed | Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
| title_short | Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
| title_sort | metallothionein 1 and nitric oxide expression are inversely correlated in a murine model of chagas disease |
| topic | metallothionein-I antioxidants nitric oxide L-NAME Chagas disease |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174&lng=en&tlng=en |
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