In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors
During product isolation the received bioreceptors often do not exhibit a sufficient biochemical activity due to multistep dissociation and loss of cofactors. However, for bioelectrochemical applications the presence of cofactors is necessary for a successful oxidative or reductive conversion of the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Computational and Structural Biotechnology Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037022002926 |
| _version_ | 1797978204577726464 |
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| author | N. Apushkinskaya E.V. Zolotukhina E.V. Butyrskaya Y.E. Silina |
| author_facet | N. Apushkinskaya E.V. Zolotukhina E.V. Butyrskaya Y.E. Silina |
| author_sort | N. Apushkinskaya |
| collection | DOAJ |
| description | During product isolation the received bioreceptors often do not exhibit a sufficient biochemical activity due to multistep dissociation and loss of cofactors. However, for bioelectrochemical applications the presence of cofactors is necessary for a successful oxidative or reductive conversion of the substrates to the products.Herein, we show how the immobilization of the required electroplated cofactors in a design of amperometric electrodes can in situ assist the activity of apo-enzymes. Compared to conventional approaches used in enzyme engineering this tailored nanoengineering methodology is superior from economic point of view, labor and time costs, storage conditions, reduced amount of waste and can fill the gap in the development of tuned bioelectrocatalysts. |
| first_indexed | 2024-04-11T05:19:15Z |
| format | Article |
| id | doaj.art-2ac1d88b8c0743cb90eeafc120d1d5a0 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-04-11T05:19:15Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-2ac1d88b8c0743cb90eeafc120d1d5a02022-12-24T04:53:24ZengElsevierComputational and Structural Biotechnology Journal2001-03702022-01-012038243832In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactorsN. Apushkinskaya0E.V. Zolotukhina1E.V. Butyrskaya2Y.E. Silina3Institute of Biochemistry, Saarland University, 66123, Saarbrücken, Campus B 2.2, GermanyInstitute of Problems of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Moscow Region, RussiaDepartment of Chemistry, Voronezh State University, Universitetskaya pl. 1, 394006, Voronezh, RussiaInstitute of Biochemistry, Saarland University, 66123, Saarbrücken, Campus B 2.2, Germany; Corresponding author at: Institute of Biochemistry, University of Saarland, Campus B 2.2, room 317, Germany.During product isolation the received bioreceptors often do not exhibit a sufficient biochemical activity due to multistep dissociation and loss of cofactors. However, for bioelectrochemical applications the presence of cofactors is necessary for a successful oxidative or reductive conversion of the substrates to the products.Herein, we show how the immobilization of the required electroplated cofactors in a design of amperometric electrodes can in situ assist the activity of apo-enzymes. Compared to conventional approaches used in enzyme engineering this tailored nanoengineering methodology is superior from economic point of view, labor and time costs, storage conditions, reduced amount of waste and can fill the gap in the development of tuned bioelectrocatalysts.http://www.sciencedirect.com/science/article/pii/S2001037022002926In situ properties modulationApo-enzymesCofactorsImmobilizationHeterogeneous biocatalysis |
| spellingShingle | N. Apushkinskaya E.V. Zolotukhina E.V. Butyrskaya Y.E. Silina In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors Computational and Structural Biotechnology Journal In situ properties modulation Apo-enzymes Cofactors Immobilization Heterogeneous biocatalysis |
| title | In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors |
| title_full | In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors |
| title_fullStr | In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors |
| title_full_unstemmed | In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors |
| title_short | In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors |
| title_sort | in situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors |
| topic | In situ properties modulation Apo-enzymes Cofactors Immobilization Heterogeneous biocatalysis |
| url | http://www.sciencedirect.com/science/article/pii/S2001037022002926 |
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