Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation
The activity of the terpenes and Cannabidiol (CBD) against human coronavirus (HCoV) strain OC43 and influenza A (H1N1) was evaluated in human lung fibroblasts (MRC-5 cells). Also, we examined whether these ingredients inhibit pro-inflammatory cytokines in peripheral blood mononuclear cells (PBMC). T...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X2400002X |
| _version_ | 1797272470699376640 |
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| author | Lior Chatow Adi Nudel Nadav Eyal Tal Lupo Silvia Ramirez Einat Zelinger Iris Nesher Richard Boxer |
| author_facet | Lior Chatow Adi Nudel Nadav Eyal Tal Lupo Silvia Ramirez Einat Zelinger Iris Nesher Richard Boxer |
| author_sort | Lior Chatow |
| collection | DOAJ |
| description | The activity of the terpenes and Cannabidiol (CBD) against human coronavirus (HCoV) strain OC43 and influenza A (H1N1) was evaluated in human lung fibroblasts (MRC-5 cells). Also, we examined whether these ingredients inhibit pro-inflammatory cytokines in peripheral blood mononuclear cells (PBMC). The tested preparations exhibited both anti-inflammatory and antiviral effects. The combination of terpenes was effective against both HCoV-OC43 and influenza A (H1N1) virus. The addition of CBD improved the antiviral activity in some, but not all cases. This variation in activity may suggest an antiviral mechanism. In addition, there was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect after 72 h, as observed under a microscope. The anti-inflammatory properties of terpenes were demonstrated using a pro-inflammatory cytokine-inhibition assay, which revealed significant cytokine inhibition and enhanced by the addition of CBD. |
| first_indexed | 2024-03-07T14:28:48Z |
| format | Article |
| id | doaj.art-2ac3505cd90b494ba336f844fe1f1bb6 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-03-07T14:28:48Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-2ac3505cd90b494ba336f844fe1f1bb62024-03-06T05:27:10ZengElsevierBiotechnology Reports2215-017X2024-03-0141e00829Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluationLior Chatow0Adi Nudel1Nadav Eyal2Tal Lupo3Silvia Ramirez4Einat Zelinger5Iris Nesher6Richard Boxer7Eybna Technologies Ltd., Kfar Saba, IsraelEybna Technologies Ltd., Kfar Saba, IsraelEybna Technologies Ltd., Kfar Saba, IsraelCorresponding author.; Eybna Technologies Ltd., Kfar Saba, IsraelEybna Technologies Ltd., Kfar Saba, IsraelCSI Center for Scientific Imaging Faculty of Agriculture, Hebrew University, Rehovot, IsraelEybna Technologies Ltd., Kfar Saba, IsraelDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USAThe activity of the terpenes and Cannabidiol (CBD) against human coronavirus (HCoV) strain OC43 and influenza A (H1N1) was evaluated in human lung fibroblasts (MRC-5 cells). Also, we examined whether these ingredients inhibit pro-inflammatory cytokines in peripheral blood mononuclear cells (PBMC). The tested preparations exhibited both anti-inflammatory and antiviral effects. The combination of terpenes was effective against both HCoV-OC43 and influenza A (H1N1) virus. The addition of CBD improved the antiviral activity in some, but not all cases. This variation in activity may suggest an antiviral mechanism. In addition, there was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect after 72 h, as observed under a microscope. The anti-inflammatory properties of terpenes were demonstrated using a pro-inflammatory cytokine-inhibition assay, which revealed significant cytokine inhibition and enhanced by the addition of CBD.http://www.sciencedirect.com/science/article/pii/S2215017X2400002XTerpenesCBDCoronavirusInfluenza virusCytokine storm |
| spellingShingle | Lior Chatow Adi Nudel Nadav Eyal Tal Lupo Silvia Ramirez Einat Zelinger Iris Nesher Richard Boxer Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation Biotechnology Reports Terpenes CBD Coronavirus Influenza virus Cytokine storm |
| title | Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation |
| title_full | Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation |
| title_fullStr | Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation |
| title_full_unstemmed | Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation |
| title_short | Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation |
| title_sort | terpenes and cannabidiol against human corona and influenza viruses anti inflammatory and antiviral in vitro evaluation |
| topic | Terpenes CBD Coronavirus Influenza virus Cytokine storm |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X2400002X |
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