Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses
NAC transcription factor is one of the largest plant gene families, participating in the regulation of plant biological and abiotic stresses. In this study, 182 NAC proteins (HhNACs) were identified based on genomic datasets of <i>Hibiscus hamabo</i> Sieb. et Zucc (<i>H. hamabo<...
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/6/3055 |
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| author | Zhiquan Wang Longjie Ni Dina Liu Zekai Fu Jianfeng Hua Zhiguo Lu Liangqin Liu Yunlong Yin Huogen Li Chunsun Gu |
| author_facet | Zhiquan Wang Longjie Ni Dina Liu Zekai Fu Jianfeng Hua Zhiguo Lu Liangqin Liu Yunlong Yin Huogen Li Chunsun Gu |
| author_sort | Zhiquan Wang |
| collection | DOAJ |
| description | NAC transcription factor is one of the largest plant gene families, participating in the regulation of plant biological and abiotic stresses. In this study, 182 NAC proteins (HhNACs) were identified based on genomic datasets of <i>Hibiscus hamabo</i> Sieb. et Zucc (<i>H. hamabo</i>). These proteins were divided into 19 subfamilies based on their phylogenetic relationship, motif pattern, and gene structure analysis. Expression analysis with RNA-seq revealed that most <i>HhNACs</i> were expressed in response to drought and salt stress. Research of quantitative real-time PCR analysis of nine selected <i>HhNACs</i> supported the transcriptome data’s dependability and suggested that <i>HhNAC54</i> was significantly upregulated under multiple abiotic stresses. Overexpression of <i>HhNAC54</i> in <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) significantly increased its tolerance to salt. This study provides a basis for a comprehensive analysis of NAC transcription factor and insight into the abiotic stress response mechanism in <i>H. hamabo</i>. |
| first_indexed | 2024-03-09T19:43:08Z |
| format | Article |
| id | doaj.art-2ac5a961c09b43e29b759ad7f3a1f6dd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:43:08Z |
| publishDate | 2022-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2ac5a961c09b43e29b759ad7f3a1f6dd2023-11-24T01:31:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236305510.3390/ijms23063055Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic StressesZhiquan Wang0Longjie Ni1Dina Liu2Zekai Fu3Jianfeng Hua4Zhiguo Lu5Liangqin Liu6Yunlong Yin7Huogen Li8Chunsun Gu9Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaCollege of Forest Sciences, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Forest Sciences, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Forest Sciences, Nanjing Forestry University, Nanjing 210037, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaCollege of Forest Sciences, Nanjing Forestry University, Nanjing 210037, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaNAC transcription factor is one of the largest plant gene families, participating in the regulation of plant biological and abiotic stresses. In this study, 182 NAC proteins (HhNACs) were identified based on genomic datasets of <i>Hibiscus hamabo</i> Sieb. et Zucc (<i>H. hamabo</i>). These proteins were divided into 19 subfamilies based on their phylogenetic relationship, motif pattern, and gene structure analysis. Expression analysis with RNA-seq revealed that most <i>HhNACs</i> were expressed in response to drought and salt stress. Research of quantitative real-time PCR analysis of nine selected <i>HhNACs</i> supported the transcriptome data’s dependability and suggested that <i>HhNAC54</i> was significantly upregulated under multiple abiotic stresses. Overexpression of <i>HhNAC54</i> in <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) significantly increased its tolerance to salt. This study provides a basis for a comprehensive analysis of NAC transcription factor and insight into the abiotic stress response mechanism in <i>H. hamabo</i>.https://www.mdpi.com/1422-0067/23/6/3055NAC familytranscription factorsabiotic stresssemi-mangrove plant<i>Hibiscus hamabo</i> |
| spellingShingle | Zhiquan Wang Longjie Ni Dina Liu Zekai Fu Jianfeng Hua Zhiguo Lu Liangqin Liu Yunlong Yin Huogen Li Chunsun Gu Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses International Journal of Molecular Sciences NAC family transcription factors abiotic stress semi-mangrove plant <i>Hibiscus hamabo</i> |
| title | Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses |
| title_full | Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses |
| title_fullStr | Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses |
| title_full_unstemmed | Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses |
| title_short | Genome-Wide Identification and Characterization of NAC Family in <i>Hibiscus hamabo</i> Sieb. et Zucc. under Various Abiotic Stresses |
| title_sort | genome wide identification and characterization of nac family in i hibiscus hamabo i sieb et zucc under various abiotic stresses |
| topic | NAC family transcription factors abiotic stress semi-mangrove plant <i>Hibiscus hamabo</i> |
| url | https://www.mdpi.com/1422-0067/23/6/3055 |
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