Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks
This manuscript describes the analysis of xylanase production by Aspergillus niger NRRL 328 in solid state fermentation (SSF) of soybean husks. A maximum value of extracellular xylanase activity of approximately 950 U g-1 was achieved after 96 h. Proteomic analyses performed on the enzymatic mixture...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-10-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_Weingartner_Hemicellulolytic_Enzymes |
| _version_ | 1819091379271237632 |
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| author | Valesca Weingartner Montibeller Luciana Porto de Souza Vandenberghe Antonella Amore Carlos Ricardo Soccol Leila Birolo Roberto Vinciguerra Denise Naomi Xavier Salmon Michele Rigon Spier Vincenza Faraco |
| author_facet | Valesca Weingartner Montibeller Luciana Porto de Souza Vandenberghe Antonella Amore Carlos Ricardo Soccol Leila Birolo Roberto Vinciguerra Denise Naomi Xavier Salmon Michele Rigon Spier Vincenza Faraco |
| author_sort | Valesca Weingartner Montibeller |
| collection | DOAJ |
| description | This manuscript describes the analysis of xylanase production by Aspergillus niger NRRL 328 in solid state fermentation (SSF) of soybean husks. A maximum value of extracellular xylanase activity of approximately 950 U g-1 was achieved after 96 h. Proteomic analyses performed on the enzymatic mixture responsible for the maximum value of xylanase activity in SSF revealed the presence of two xylanases. This xylanolytic mixture was partially purified and characterized. It followed Michaelis-Menten kinetics towards xylan, with a KM of 7.92 ±0.97 mg xylan/mL and a Vmax of 262.2 ± 27.8 g L-1 s-1. The optimum pH for the enzyme is 5.3, and the optimal temperature is 50 °C. The enzyme retains 100% of its activity at 40 °C for at least 1 month. It shows very high stability in a broad pH range, with a half-life of 40 days at pH 5.3, pH 6.0, pH 6.5, pH 7.0, and pH 8.0. |
| first_indexed | 2024-12-21T22:38:47Z |
| format | Article |
| id | doaj.art-2aca341a7ddb4cf0a106ddbdf196b961 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-21T22:38:47Z |
| publishDate | 2014-10-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-2aca341a7ddb4cf0a106ddbdf196b9612022-12-21T18:47:54ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-10-01947128714010.15376/biores.9.4.7128-7140Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean HusksValesca Weingartner Montibeller0Luciana Porto de Souza Vandenberghe1Antonella Amore2Carlos Ricardo Soccol3Leila Birolo4Roberto Vinciguerra5Denise Naomi Xavier Salmon6Michele Rigon Spier7Vincenza Faraco8Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; BrazilDepartment of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; ItalyDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; BrazilDepartment of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; ItalyDepartment of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; ItalyDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; BrazilDepartment of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; ItalyThis manuscript describes the analysis of xylanase production by Aspergillus niger NRRL 328 in solid state fermentation (SSF) of soybean husks. A maximum value of extracellular xylanase activity of approximately 950 U g-1 was achieved after 96 h. Proteomic analyses performed on the enzymatic mixture responsible for the maximum value of xylanase activity in SSF revealed the presence of two xylanases. This xylanolytic mixture was partially purified and characterized. It followed Michaelis-Menten kinetics towards xylan, with a KM of 7.92 ±0.97 mg xylan/mL and a Vmax of 262.2 ± 27.8 g L-1 s-1. The optimum pH for the enzyme is 5.3, and the optimal temperature is 50 °C. The enzyme retains 100% of its activity at 40 °C for at least 1 month. It shows very high stability in a broad pH range, with a half-life of 40 days at pH 5.3, pH 6.0, pH 6.5, pH 7.0, and pH 8.0.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_Weingartner_Hemicellulolytic_EnzymesAspergillus nigerSolid state fermentationXylanase |
| spellingShingle | Valesca Weingartner Montibeller Luciana Porto de Souza Vandenberghe Antonella Amore Carlos Ricardo Soccol Leila Birolo Roberto Vinciguerra Denise Naomi Xavier Salmon Michele Rigon Spier Vincenza Faraco Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks BioResources Aspergillus niger Solid state fermentation Xylanase |
| title | Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks |
| title_full | Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks |
| title_fullStr | Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks |
| title_full_unstemmed | Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks |
| title_short | Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks |
| title_sort | characterization of hemicellulolytic enzymes produced by aspergillus niger nrrl 328 under solid state fermentation on soybean husks |
| topic | Aspergillus niger Solid state fermentation Xylanase |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_Weingartner_Hemicellulolytic_Enzymes |
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