Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases
Azurophilic granulation of blasts is a feature of acute myeloid leukemia (AML). Granular acute lymphoblastic leukemia (ALL) may mimic AML due to the presence of cytoplasmic granules in lymphoblasts, but cytochemistry and immunophenotyping are helpful in making the correct diagnosis. Toluidine blue (...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2010-03-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | http://www.journalagent.com/z4/download_fulltext.asp?pdir=tjh&plng=eng&un=TJH-53825 |
| _version_ | 1797918409443246080 |
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| author | Rishu Agarwal Ritu Gupta Sameer Bakhshi Atul Sharma |
| author_facet | Rishu Agarwal Ritu Gupta Sameer Bakhshi Atul Sharma |
| author_sort | Rishu Agarwal |
| collection | DOAJ |
| description | Azurophilic granulation of blasts is a feature of acute myeloid leukemia (AML). Granular acute lymphoblastic leukemia (ALL) may mimic AML due to the presence of cytoplasmic granules in lymphoblasts, but cytochemistry and immunophenotyping are helpful in making the correct diagnosis. Toluidine blue (TB) is a metachromatic dye, which stains basophils and myeloid blasts that exhibit basophilic differentiation. Reactivity for TB has not been described in lymphoblasts. We herein report two cases of granular ALL with blasts exhibiting reactivity for TB that caused diagnostic dilemma. Immunophenotyping and cytogenetic studies were helpful in making a correct diagnosis. This report of two rare case highlight the reactivity of lymphoblasts with TB not hitherto described and the importance of a detailed diagnostic work-up in acute leukemia. |
| first_indexed | 2024-04-10T13:28:52Z |
| format | Article |
| id | doaj.art-2acbbda9f9f7451392713b827bbf5b0b |
| institution | Directory Open Access Journal |
| issn | 1300-7777 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T13:28:52Z |
| publishDate | 2010-03-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-2acbbda9f9f7451392713b827bbf5b0b2023-02-15T16:11:45ZengGalenos Publishing HouseTurkish Journal of Hematology1300-77771308-52632010-03-0127014345Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare casesRishu AgarwalRitu GuptaSameer BakhshiAtul SharmaAzurophilic granulation of blasts is a feature of acute myeloid leukemia (AML). Granular acute lymphoblastic leukemia (ALL) may mimic AML due to the presence of cytoplasmic granules in lymphoblasts, but cytochemistry and immunophenotyping are helpful in making the correct diagnosis. Toluidine blue (TB) is a metachromatic dye, which stains basophils and myeloid blasts that exhibit basophilic differentiation. Reactivity for TB has not been described in lymphoblasts. We herein report two cases of granular ALL with blasts exhibiting reactivity for TB that caused diagnostic dilemma. Immunophenotyping and cytogenetic studies were helpful in making a correct diagnosis. This report of two rare case highlight the reactivity of lymphoblasts with TB not hitherto described and the importance of a detailed diagnostic work-up in acute leukemia.http://www.journalagent.com/z4/download_fulltext.asp?pdir=tjh&plng=eng&un=TJH-53825Granular ALLbasophilic leukemiatoluidine blueazurophilic granules |
| spellingShingle | Rishu Agarwal Ritu Gupta Sameer Bakhshi Atul Sharma Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases Turkish Journal of Hematology Granular ALL basophilic leukemia toluidine blue azurophilic granules |
| title | Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases |
| title_full | Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases |
| title_fullStr | Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases |
| title_full_unstemmed | Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases |
| title_short | Unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia: a report of two rare cases |
| title_sort | unusual cytochemical reactivity for toluidine blue in granular acute lymphoblastic leukemia a report of two rare cases |
| topic | Granular ALL basophilic leukemia toluidine blue azurophilic granules |
| url | http://www.journalagent.com/z4/download_fulltext.asp?pdir=tjh&plng=eng&un=TJH-53825 |
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