A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato
With global warming and water shortage, drought stress is provoking an increasing impact on plant growth, development, and crop productivity worldwide. Pipecolic acid (Pip) is an emerging lysine catabolite in plants, acting as a critical element in disease resistance with a related signal pathway of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/12/1923 |
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| author | Ping Wang Qian Luo Weicheng Yang Golam Jalal Ahammed Shuting Ding Xingyu Chen Jiao Wang Xiaojian Xia Kai Shi |
| author_facet | Ping Wang Qian Luo Weicheng Yang Golam Jalal Ahammed Shuting Ding Xingyu Chen Jiao Wang Xiaojian Xia Kai Shi |
| author_sort | Ping Wang |
| collection | DOAJ |
| description | With global warming and water shortage, drought stress is provoking an increasing impact on plant growth, development, and crop productivity worldwide. Pipecolic acid (Pip) is an emerging lysine catabolite in plants, acting as a critical element in disease resistance with a related signal pathway of phytohormone salicylic acid (SA). While SA plays a vital role in various abiotic stresses, the role of Pip in plant response to abiotic stresses, especially drought, remains largely unknown. To address this issue, Pip biosynthetic gene <i>Slald1</i> mutants and hydroxylated modification gene <i>Slfmo1</i> mutants were generated using CRISPR-Cas9 gene-editing approaches. Drought resistance dramatically increased in <i>Slald1</i> mutants compared with wild-type, which was associated with increased CO<sub>2</sub> assimilation, photosystems activities, antioxidant enzymes activities, ascorbate and glutathione content, and reduced reactive oxygen species accumulation, lipid peroxidation and protein oxidation. On the contrary, <i>Slfmo1</i> mutants were more sensitive to drought, showing damaged photosystems and impaired antioxidant systems, which were significantly alleviated by exogenous ascorbate. Our results demonstrate that Pip biosynthesis and hydroxylated modification pathways play a critical role in drought tolerance through the antioxidant system in tomato. This knowledge can be helpful to breed improved crop cultivars that are better equipped with drought resistance. |
| first_indexed | 2024-03-10T04:38:19Z |
| format | Article |
| id | doaj.art-2acc78c8331b421fb48293cc522946d0 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T04:38:19Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-2acc78c8331b421fb48293cc522946d02023-11-23T03:32:59ZengMDPI AGAntioxidants2076-39212021-11-011012192310.3390/antiox10121923A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in TomatoPing Wang0Qian Luo1Weicheng Yang2Golam Jalal Ahammed3Shuting Ding4Xingyu Chen5Jiao Wang6Xiaojian Xia7Kai Shi8Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, ChinaWith global warming and water shortage, drought stress is provoking an increasing impact on plant growth, development, and crop productivity worldwide. Pipecolic acid (Pip) is an emerging lysine catabolite in plants, acting as a critical element in disease resistance with a related signal pathway of phytohormone salicylic acid (SA). While SA plays a vital role in various abiotic stresses, the role of Pip in plant response to abiotic stresses, especially drought, remains largely unknown. To address this issue, Pip biosynthetic gene <i>Slald1</i> mutants and hydroxylated modification gene <i>Slfmo1</i> mutants were generated using CRISPR-Cas9 gene-editing approaches. Drought resistance dramatically increased in <i>Slald1</i> mutants compared with wild-type, which was associated with increased CO<sub>2</sub> assimilation, photosystems activities, antioxidant enzymes activities, ascorbate and glutathione content, and reduced reactive oxygen species accumulation, lipid peroxidation and protein oxidation. On the contrary, <i>Slfmo1</i> mutants were more sensitive to drought, showing damaged photosystems and impaired antioxidant systems, which were significantly alleviated by exogenous ascorbate. Our results demonstrate that Pip biosynthesis and hydroxylated modification pathways play a critical role in drought tolerance through the antioxidant system in tomato. This knowledge can be helpful to breed improved crop cultivars that are better equipped with drought resistance.https://www.mdpi.com/2076-3921/10/12/1923pipecolic acidCRISPR-Cas9drought resistancephotosystemsantioxidants |
| spellingShingle | Ping Wang Qian Luo Weicheng Yang Golam Jalal Ahammed Shuting Ding Xingyu Chen Jiao Wang Xiaojian Xia Kai Shi A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato Antioxidants pipecolic acid CRISPR-Cas9 drought resistance photosystems antioxidants |
| title | A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato |
| title_full | A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato |
| title_fullStr | A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato |
| title_full_unstemmed | A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato |
| title_short | A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato |
| title_sort | novel role of pipecolic acid biosynthetic pathway in drought tolerance through the antioxidant system in tomato |
| topic | pipecolic acid CRISPR-Cas9 drought resistance photosystems antioxidants |
| url | https://www.mdpi.com/2076-3921/10/12/1923 |
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