Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran
Abstract Background Field-testing genetically modified crops provides scientists with an opportunity to collect information on environmental interactions and agronomic performance, which is critical to a full environmental safety assessment as required by regulatory authorities. As a result, the goa...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s43141-020-00028-8 |
| _version_ | 1797204992135790592 |
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| author | Salman Dastan Behzad Ghareyazie Shahpour Abdollahi |
| author_facet | Salman Dastan Behzad Ghareyazie Shahpour Abdollahi |
| author_sort | Salman Dastan |
| collection | DOAJ |
| description | Abstract Background Field-testing genetically modified crops provides scientists with an opportunity to collect information on environmental interactions and agronomic performance, which is critical to a full environmental safety assessment as required by regulatory authorities. As a result, the goal of this research was field trial of transgenic and conventional rice genotypes. The experiment was carried out in a randomized complete blocks design (RCBD) with four replications and seven genotypes in three isolated regions under the biosafety standard protocol in north of Iran in 2016. In this study, four transgenic lines with an active cry1Ab gene in the vegetative stage and three conventional genotypes (control) as treatment were assessed. Results The findings demonstrated that in all three regions, transgenic lines derived from Khazar cultivar, were similar to their parent(s) in terms of growth phenology, agronomical traits, grain amylose content, gel consistency, and gelatinization temperature. In all the three regions, the highest number of panicle per m2, number of filled spikelet per panicle, and filled spikelet percentage per panicle were obtained for transgenic lines. Khazar cultivar compared to the transgenic lines showed lower paddy yield. In all the three regions, transgenic lines had lower yield loss than their non-transgenic parent. The lowest number of white heads belonged to transgenic Tarom Molaii and transgenic lines. The most positive direct effect on paddy yield was related to the number of filled spikelet per panicle. Thus, this can be a good trait to achieve higher yield derived from reducing the negative indirect effect of dead heart and white heads. Conclusion It can be concluded that by producing transgenic rice, which is resistant to stem borer with an active promoter in the reproductive stage, farmers can reduce significant part of yield loss resulting from dead heart and white heads directly correlating with the number of filled spikelet per panicle and paddy yield. |
| first_indexed | 2024-04-24T08:44:02Z |
| format | Article |
| id | doaj.art-2ad5a8b379f24f7eb5a42d9cd2e10af0 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:44:02Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-2ad5a8b379f24f7eb5a42d9cd2e10af02024-04-16T14:09:20ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202020-05-0118111410.1186/s43141-020-00028-8Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of IranSalman Dastan0Behzad Ghareyazie1Shahpour Abdollahi2Department of Biosafety and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII)Department of Biosafety and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII)Department of Biosafety and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII)Abstract Background Field-testing genetically modified crops provides scientists with an opportunity to collect information on environmental interactions and agronomic performance, which is critical to a full environmental safety assessment as required by regulatory authorities. As a result, the goal of this research was field trial of transgenic and conventional rice genotypes. The experiment was carried out in a randomized complete blocks design (RCBD) with four replications and seven genotypes in three isolated regions under the biosafety standard protocol in north of Iran in 2016. In this study, four transgenic lines with an active cry1Ab gene in the vegetative stage and three conventional genotypes (control) as treatment were assessed. Results The findings demonstrated that in all three regions, transgenic lines derived from Khazar cultivar, were similar to their parent(s) in terms of growth phenology, agronomical traits, grain amylose content, gel consistency, and gelatinization temperature. In all the three regions, the highest number of panicle per m2, number of filled spikelet per panicle, and filled spikelet percentage per panicle were obtained for transgenic lines. Khazar cultivar compared to the transgenic lines showed lower paddy yield. In all the three regions, transgenic lines had lower yield loss than their non-transgenic parent. The lowest number of white heads belonged to transgenic Tarom Molaii and transgenic lines. The most positive direct effect on paddy yield was related to the number of filled spikelet per panicle. Thus, this can be a good trait to achieve higher yield derived from reducing the negative indirect effect of dead heart and white heads. Conclusion It can be concluded that by producing transgenic rice, which is resistant to stem borer with an active promoter in the reproductive stage, farmers can reduce significant part of yield loss resulting from dead heart and white heads directly correlating with the number of filled spikelet per panicle and paddy yield.http://link.springer.com/article/10.1186/s43141-020-00028-8Bacillus thuringiensiscry1Ab geneGM riceStem borerWhite headsYield loss |
| spellingShingle | Salman Dastan Behzad Ghareyazie Shahpour Abdollahi Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran Journal of Genetic Engineering and Biotechnology Bacillus thuringiensis cry1Ab gene GM rice Stem borer White heads Yield loss |
| title | Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran |
| title_full | Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran |
| title_fullStr | Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran |
| title_full_unstemmed | Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran |
| title_short | Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran |
| title_sort | field trial evidence of non transgenic and transgenic bt rice genotypes in north of iran |
| topic | Bacillus thuringiensis cry1Ab gene GM rice Stem borer White heads Yield loss |
| url | http://link.springer.com/article/10.1186/s43141-020-00028-8 |
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