Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller)
Queensley Eghianruwa,1,2 Omolaja Osoniyi,3 Naomi Maina,1,4 Sabina Wachira,5 Mabel Imbuga4 1Department of Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation, JKUAT campus, Juja, Kenya; 2Department of Biochemistry, University of Uyo, Uyo, Nigeri...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-10-01
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| Series: | Journal of Pain Research |
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| Online Access: | https://www.dovepress.com/evaluation-of-analgesic-activities-of-extracts-of-two-marine-molluscs--peer-reviewed-article-JPR |
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| author | Eghianruwa Q Osoniyi O Maina N Wachira S Imbuga M |
| author_facet | Eghianruwa Q Osoniyi O Maina N Wachira S Imbuga M |
| author_sort | Eghianruwa Q |
| collection | DOAJ |
| description | Queensley Eghianruwa,1,2 Omolaja Osoniyi,3 Naomi Maina,1,4 Sabina Wachira,5 Mabel Imbuga4 1Department of Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation, JKUAT campus, Juja, Kenya; 2Department of Biochemistry, University of Uyo, Uyo, Nigeria; 3Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile Ife, Nigeria; 4Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya; 5Centre for Traditional Medicine and Drug Research, Kenya Medical Research Institute, Nairobi, KenyaCorrespondence: Queensley EghianruwaDepartment of Biochemistry, University of Uyo, Uyo, NigeriaTel +2348024195501Email qaeghianruwa@uniuyo.edu.ngPurpose and Methods: In this study, the analgesic activity of the crude alcohol (acetone-methanol) and aqueous (in PBS, pH 7.2) extracts of the marine molluscs, Pachymelania aurita and Tympanotonus fuscatus, has been evaluated using the formalin test (for chronic antinociceptive) and the tail-flick (acute antinociceptive) pain models in male swiss albino mice.Results: The results show that the extracts of P. aurita and T. fuscatus demonstrated high safety margins as single doses of up to 2000 mg/kg bwt proved to be well tolerated and non-lethal, although the alcohol extract of P. aurita caused necrosis in the liver and kidney when administered at a dose level of 2000 mg/kg bwt. In the formalin test, treatment with the aqueous extracts of P. aurita and T. fuscatus as well as the alcohol extract of T. fuscatus 30 min before the subcutaneous injection of 5% formalin to the paw of the mice resulted in a significant time- and dose-dependent reduction in total and phase 2a pain-related behavior and thus nociception. The extracts had no analgesic effect in tail-flick test up to the highest dose tested.Conclusion: Hence, the results from both models indicate that the site of their analgesic action is probably peripheral.Keywords: Nigerian periwinkle, formalin test, anti-nociception and analgesia, marine natural products, bioactivity |
| first_indexed | 2024-12-12T05:48:04Z |
| format | Article |
| id | doaj.art-2ad5afe6daa84ea2acb6e4cf073c2711 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-12-12T05:48:04Z |
| publishDate | 2020-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Pain Research |
| spelling | doaj.art-2ad5afe6daa84ea2acb6e4cf073c27112022-12-22T00:35:45ZengDove Medical PressJournal of Pain Research1178-70902020-10-01Volume 132739274758734Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller)Eghianruwa QOsoniyi OMaina NWachira SImbuga MQueensley Eghianruwa,1,2 Omolaja Osoniyi,3 Naomi Maina,1,4 Sabina Wachira,5 Mabel Imbuga4 1Department of Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation, JKUAT campus, Juja, Kenya; 2Department of Biochemistry, University of Uyo, Uyo, Nigeria; 3Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile Ife, Nigeria; 4Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya; 5Centre for Traditional Medicine and Drug Research, Kenya Medical Research Institute, Nairobi, KenyaCorrespondence: Queensley EghianruwaDepartment of Biochemistry, University of Uyo, Uyo, NigeriaTel +2348024195501Email qaeghianruwa@uniuyo.edu.ngPurpose and Methods: In this study, the analgesic activity of the crude alcohol (acetone-methanol) and aqueous (in PBS, pH 7.2) extracts of the marine molluscs, Pachymelania aurita and Tympanotonus fuscatus, has been evaluated using the formalin test (for chronic antinociceptive) and the tail-flick (acute antinociceptive) pain models in male swiss albino mice.Results: The results show that the extracts of P. aurita and T. fuscatus demonstrated high safety margins as single doses of up to 2000 mg/kg bwt proved to be well tolerated and non-lethal, although the alcohol extract of P. aurita caused necrosis in the liver and kidney when administered at a dose level of 2000 mg/kg bwt. In the formalin test, treatment with the aqueous extracts of P. aurita and T. fuscatus as well as the alcohol extract of T. fuscatus 30 min before the subcutaneous injection of 5% formalin to the paw of the mice resulted in a significant time- and dose-dependent reduction in total and phase 2a pain-related behavior and thus nociception. The extracts had no analgesic effect in tail-flick test up to the highest dose tested.Conclusion: Hence, the results from both models indicate that the site of their analgesic action is probably peripheral.Keywords: Nigerian periwinkle, formalin test, anti-nociception and analgesia, marine natural products, bioactivityhttps://www.dovepress.com/evaluation-of-analgesic-activities-of-extracts-of-two-marine-molluscs--peer-reviewed-article-JPRnigerian periwinkleformalin testanti-nociception and analgesiamarine natural productsbioactivity |
| spellingShingle | Eghianruwa Q Osoniyi O Maina N Wachira S Imbuga M Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller) Journal of Pain Research nigerian periwinkle formalin test anti-nociception and analgesia marine natural products bioactivity |
| title | Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller) |
| title_full | Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller) |
| title_fullStr | Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller) |
| title_full_unstemmed | Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller) |
| title_short | Evaluation of Analgesic Activities of Extracts of Two Marine Molluscs: Tympanotonus fuscatus var radula (Linnaeus) and Pachymelania aurita (Müller) |
| title_sort | evaluation of analgesic activities of extracts of two marine molluscs tympanotonus fuscatus var radula linnaeus and pachymelania aurita m uuml ller |
| topic | nigerian periwinkle formalin test anti-nociception and analgesia marine natural products bioactivity |
| url | https://www.dovepress.com/evaluation-of-analgesic-activities-of-extracts-of-two-marine-molluscs--peer-reviewed-article-JPR |
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