Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes.
<h4>Objective</h4>To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM).<h4>Method</...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0245937 |
| _version_ | 1818919774524014592 |
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| author | Marian Kacerovsky Lenka Pliskova Radka Bolehovska Daniel Lesko Romana Gerychova Petr Janku Petr Matlak Ondrej Simetka Jaroslav Stranik Tomas Faist Jan Mls Peter Vescicik Bo Jacobsson Ivana Musilova |
| author_facet | Marian Kacerovsky Lenka Pliskova Radka Bolehovska Daniel Lesko Romana Gerychova Petr Janku Petr Matlak Ondrej Simetka Jaroslav Stranik Tomas Faist Jan Mls Peter Vescicik Bo Jacobsson Ivana Musilova |
| author_sort | Marian Kacerovsky |
| collection | DOAJ |
| description | <h4>Objective</h4>To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM).<h4>Method</h4>In total, 405 women with singleton pregnancies complicated with PPROM were included. Cervical fluid and amniotic fluid samples were collected at the time of admission. Bacterial and G. vaginalis DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of G. vaginalis DNA ≥ 1% of the total cervical bacterial DNA were used to define the cervical prevalence of G. vaginalis as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra-amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI.<h4>Results</h4>The presence of the abundant cervical G. vaginalis was related to MIAC (with: 65% vs. without: 44%; p = 0.0004) but not IAI (with: 52% vs. without: 48%; p = 0.70). Women with MIAC without IAI had the highest load of the cervical G. vaginalis DNA (median 2.0 × 104 copies DNA/mL) and the highest presence of abundant cervical G. vaginalis (73%).<h4>Conclusions</h4>In women with PPROM, the presence of cervical G. vaginalis was associated with MIAC, mainly without the concurrent presence of IAI. |
| first_indexed | 2024-12-20T01:11:12Z |
| format | Article |
| id | doaj.art-2ad65ce1d5aa438b9f2e5dfc617c4375 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:11:12Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2ad65ce1d5aa438b9f2e5dfc617c43752022-12-21T19:58:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e024593710.1371/journal.pone.0245937Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes.Marian KacerovskyLenka PliskovaRadka BolehovskaDaniel LeskoRomana GerychovaPetr JankuPetr MatlakOndrej SimetkaJaroslav StranikTomas FaistJan MlsPeter VescicikBo JacobssonIvana Musilova<h4>Objective</h4>To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM).<h4>Method</h4>In total, 405 women with singleton pregnancies complicated with PPROM were included. Cervical fluid and amniotic fluid samples were collected at the time of admission. Bacterial and G. vaginalis DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of G. vaginalis DNA ≥ 1% of the total cervical bacterial DNA were used to define the cervical prevalence of G. vaginalis as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra-amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI.<h4>Results</h4>The presence of the abundant cervical G. vaginalis was related to MIAC (with: 65% vs. without: 44%; p = 0.0004) but not IAI (with: 52% vs. without: 48%; p = 0.70). Women with MIAC without IAI had the highest load of the cervical G. vaginalis DNA (median 2.0 × 104 copies DNA/mL) and the highest presence of abundant cervical G. vaginalis (73%).<h4>Conclusions</h4>In women with PPROM, the presence of cervical G. vaginalis was associated with MIAC, mainly without the concurrent presence of IAI.https://doi.org/10.1371/journal.pone.0245937 |
| spellingShingle | Marian Kacerovsky Lenka Pliskova Radka Bolehovska Daniel Lesko Romana Gerychova Petr Janku Petr Matlak Ondrej Simetka Jaroslav Stranik Tomas Faist Jan Mls Peter Vescicik Bo Jacobsson Ivana Musilova Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. PLoS ONE |
| title | Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. |
| title_full | Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. |
| title_fullStr | Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. |
| title_full_unstemmed | Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. |
| title_short | Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. |
| title_sort | cervical gardnerella vaginalis in women with preterm prelabor rupture of membranes |
| url | https://doi.org/10.1371/journal.pone.0245937 |
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