Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species
In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxyleste...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323004414 |
| _version_ | 1797833099196760064 |
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| author | Marija Mrdaković Aleksandra Filipović Larisa Ilijin Anja Grčić Dragana Matić Milena Vlahović Dajana Todorović Vesna Perić-Mataruga |
| author_facet | Marija Mrdaković Aleksandra Filipović Larisa Ilijin Anja Grčić Dragana Matić Milena Vlahović Dajana Todorović Vesna Perić-Mataruga |
| author_sort | Marija Mrdaković |
| collection | DOAJ |
| description | In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as their potential as biomarkers. |
| first_indexed | 2024-04-09T14:19:17Z |
| format | Article |
| id | doaj.art-2ad72b66cccd47c8a5a219bf83b61dcc |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-09T14:19:17Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-2ad72b66cccd47c8a5a219bf83b61dcc2023-05-05T04:39:42ZengElsevierEcotoxicology and Environmental Safety0147-65132023-06-01257114937Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran speciesMarija Mrdaković0Aleksandra Filipović1Larisa Ilijin2Anja Grčić3Dragana Matić4Milena Vlahović5Dajana Todorović6Vesna Perić-Mataruga7Corresponding author.; Department of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaDepartment of Insect Physiology and Biochemistry, Institute for Biological Research ''Siniša Stanković''- National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, SerbiaIn this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as their potential as biomarkers.http://www.sciencedirect.com/science/article/pii/S0147651323004414FluorantheneLymantria disparEuproctis chrysorrhoeaCarboxylesterasesAcetylcholinesteraseHsp70 |
| spellingShingle | Marija Mrdaković Aleksandra Filipović Larisa Ilijin Anja Grčić Dragana Matić Milena Vlahović Dajana Todorović Vesna Perić-Mataruga Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species Ecotoxicology and Environmental Safety Fluoranthene Lymantria dispar Euproctis chrysorrhoea Carboxylesterases Acetylcholinesterase Hsp70 |
| title | Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species |
| title_full | Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species |
| title_fullStr | Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species |
| title_full_unstemmed | Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species |
| title_short | Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species |
| title_sort | effects of dietary fluoranthene on tissue specific responses of carboxylesterases acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species |
| topic | Fluoranthene Lymantria dispar Euproctis chrysorrhoea Carboxylesterases Acetylcholinesterase Hsp70 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323004414 |
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