Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes
Abstract Background Motion quality is a critical property for essential functions. Several endogenous and exogenous factors are involved in sperm motility. Here, we measured the relative telomere length and evaluated the gene expression of its binding-proteins, shelterin complex (TRF1, TRF2, RAP1, P...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03795-x |
| _version_ | 1797630154000826368 |
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| author | Hossein Hassanpour Pezhman Mirshokraei Marzieh Salehpour Khadije Amiri Parvin Ghareghani Leila Nasiri |
| author_facet | Hossein Hassanpour Pezhman Mirshokraei Marzieh Salehpour Khadije Amiri Parvin Ghareghani Leila Nasiri |
| author_sort | Hossein Hassanpour |
| collection | DOAJ |
| description | Abstract Background Motion quality is a critical property for essential functions. Several endogenous and exogenous factors are involved in sperm motility. Here, we measured the relative telomere length and evaluated the gene expression of its binding-proteins, shelterin complex (TRF1, TRF2, RAP1, POT1, TIN2, and TPP1) in sperm of dogs using relative quantitative real-time PCR. We compared them between two sperm subpopulations with poor and good motion qualities (separated by swim-up method). Telomere shortening and alterations of shelterin gene expression result from ROS, genotoxic insults, and genetic predisposition. Results Sperm kinematic parameters were measured in two subpopulations and then telomeric index of each parameter was calculated. Telomeric index for linearity, VSL, VCL, STR, BCF, and ALH were significantly higher in sperms with good motion quality than in sperms with poor quality. We demonstrated that poor motion quality is associated with shorter telomere, higher expression of TRF2, POT1, and TIN2 genes, and lower expression of the RAP1 gene in dog sperm. The levels of TRF1 and TPP1 gene expression remained consistent despite variations in sperm quality and telomere length. Conclusion Data provided evidence that there are considerable changes in gene expression of many shelterin components (TRF2, TIN2, POT1and RAP1) associated with shortening telomere in the spermatozoa with poor motion quality. Possibly, the poor motion quality is the result of defects in the shelterin complex and telomere length. Our data suggests a new approach in the semen assessment and etiologic investigations of subfertility or infertility in male animals. |
| first_indexed | 2024-03-11T11:03:00Z |
| format | Article |
| id | doaj.art-2ad79121fa8a4ccc911ec7f17342422a |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-11T11:03:00Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-2ad79121fa8a4ccc911ec7f17342422a2023-11-12T12:20:42ZengBMCBMC Veterinary Research1746-61482023-11-011911910.1186/s12917-023-03795-xCanine sperm motility is associated with telomere shortening and changes in expression of shelterin genesHossein Hassanpour0Pezhman Mirshokraei1Marzieh Salehpour2Khadije Amiri3Parvin Ghareghani4Leila Nasiri5Department of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord UniversityDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of MashhadDepartment of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord UniversityDepartment of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord UniversityDepartment of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord UniversityDepartment of Biology, Faculty of Basic Sciences, Shahed UniversityAbstract Background Motion quality is a critical property for essential functions. Several endogenous and exogenous factors are involved in sperm motility. Here, we measured the relative telomere length and evaluated the gene expression of its binding-proteins, shelterin complex (TRF1, TRF2, RAP1, POT1, TIN2, and TPP1) in sperm of dogs using relative quantitative real-time PCR. We compared them between two sperm subpopulations with poor and good motion qualities (separated by swim-up method). Telomere shortening and alterations of shelterin gene expression result from ROS, genotoxic insults, and genetic predisposition. Results Sperm kinematic parameters were measured in two subpopulations and then telomeric index of each parameter was calculated. Telomeric index for linearity, VSL, VCL, STR, BCF, and ALH were significantly higher in sperms with good motion quality than in sperms with poor quality. We demonstrated that poor motion quality is associated with shorter telomere, higher expression of TRF2, POT1, and TIN2 genes, and lower expression of the RAP1 gene in dog sperm. The levels of TRF1 and TPP1 gene expression remained consistent despite variations in sperm quality and telomere length. Conclusion Data provided evidence that there are considerable changes in gene expression of many shelterin components (TRF2, TIN2, POT1and RAP1) associated with shortening telomere in the spermatozoa with poor motion quality. Possibly, the poor motion quality is the result of defects in the shelterin complex and telomere length. Our data suggests a new approach in the semen assessment and etiologic investigations of subfertility or infertility in male animals.https://doi.org/10.1186/s12917-023-03795-xTelomereShelterinKinematic parametersSpermGene expression |
| spellingShingle | Hossein Hassanpour Pezhman Mirshokraei Marzieh Salehpour Khadije Amiri Parvin Ghareghani Leila Nasiri Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes BMC Veterinary Research Telomere Shelterin Kinematic parameters Sperm Gene expression |
| title | Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes |
| title_full | Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes |
| title_fullStr | Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes |
| title_full_unstemmed | Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes |
| title_short | Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes |
| title_sort | canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes |
| topic | Telomere Shelterin Kinematic parameters Sperm Gene expression |
| url | https://doi.org/10.1186/s12917-023-03795-x |
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