Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands a...
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| Format: | Article |
| Language: | English |
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Embrapa Informação Tecnológica
2010-12-01
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| Series: | Pesquisa Agropecuária Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200013&lng=en&tlng=en |
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| author | Mohammad Zaefizadeh Shahzad Jamaati-e-Somarin Javid Ojaghi Seyed Mehdi Seyedi Roghayyeh Zabihi-e-Mahmoodabad Mehran Ochi |
| author_facet | Mohammad Zaefizadeh Shahzad Jamaati-e-Somarin Javid Ojaghi Seyed Mehdi Seyedi Roghayyeh Zabihi-e-Mahmoodabad Mehran Ochi |
| author_sort | Mohammad Zaefizadeh |
| collection | DOAJ |
| description | The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies |
| first_indexed | 2024-12-12T04:31:23Z |
| format | Article |
| id | doaj.art-2ad879b91c7849859333256576f705c9 |
| institution | Directory Open Access Journal |
| issn | 1678-3921 |
| language | English |
| last_indexed | 2024-12-12T04:31:23Z |
| publishDate | 2010-12-01 |
| publisher | Embrapa Informação Tecnológica |
| record_format | Article |
| series | Pesquisa Agropecuária Brasileira |
| spelling | doaj.art-2ad879b91c7849859333256576f705c92022-12-22T00:38:05ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212010-12-0145121425143210.1590/S0100-204X2010001200013S0100-204X2010001200013Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and AzerbaijanMohammad Zaefizadeh0Shahzad Jamaati-e-Somarin1Javid Ojaghi2Seyed Mehdi Seyedi3Roghayyeh Zabihi-e-Mahmoodabad4Mehran Ochi5Islamic Azad UniversityIslamic Azad UniversityBaku State University-AzerbaijanNational Institute of Genetic Engineering and BiotechnologyIslamic Azad UniversityAcademic Center for Evaluation & Researchers of IranThe objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studieshttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200013&lng=en&tlng=enTriticum durumanálise "acid PAGE"germoplasmaagricultura tradicional |
| spellingShingle | Mohammad Zaefizadeh Shahzad Jamaati-e-Somarin Javid Ojaghi Seyed Mehdi Seyedi Roghayyeh Zabihi-e-Mahmoodabad Mehran Ochi Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan Pesquisa Agropecuária Brasileira Triticum durum análise "acid PAGE" germoplasma agricultura tradicional |
| title | Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan |
| title_full | Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan |
| title_fullStr | Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan |
| title_full_unstemmed | Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan |
| title_short | Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan |
| title_sort | genetic diversity for gliadin patterns of durum wheat landraces in the northwest of iran and azerbaijan |
| topic | Triticum durum análise "acid PAGE" germoplasma agricultura tradicional |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200013&lng=en&tlng=en |
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