Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells
<i>Leptocarpha rivularis</i> is a native South American plant used ancestrally by Mapuche people to treat gastrointestinal ailments. <i>L. rivularis</i> flower extracts are rich in molecules with therapeutic potential, including the sesquiterpene lactone leptocarpin, which di...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/2/1439 |
| _version_ | 1797441268359364608 |
|---|---|
| author | Nicolás Carrasco Maritza Garrido Iván Montenegro Alejandro Madrid Ricardo Hartley Iván González Mariaignacia Rubilar Joan Villena Manuel Valenzuela-Valderrama |
| author_facet | Nicolás Carrasco Maritza Garrido Iván Montenegro Alejandro Madrid Ricardo Hartley Iván González Mariaignacia Rubilar Joan Villena Manuel Valenzuela-Valderrama |
| author_sort | Nicolás Carrasco |
| collection | DOAJ |
| description | <i>Leptocarpha rivularis</i> is a native South American plant used ancestrally by Mapuche people to treat gastrointestinal ailments. <i>L. rivularis</i> flower extracts are rich in molecules with therapeutic potential, including the sesquiterpene lactone leptocarpin, which displays cytotoxic effects against various cancer types in vitro. However, the combination of active molecules in these extracts could offer a hitherto unexplored potential for targeting cancer. In this study, we investigated the effect of <i>L. rivularis</i> flower extracts on the proliferation, survival, and spread parameters of gastric cancer cells in vitro. Gastric cancer (AGS and MKN-45) and normal immortalized (GES-1) cell lines were treated with different concentrations of <i>L. rivularis</i> flower extracts (DCM, Hex, EtOAc, and EtOH) and we determined the changes in proliferation (MTS assay, cell cycle analysis), cell viability/cytotoxicity (trypan blue exclusion assay, DEVDase activity, mitochondrial membrane potential MMP, and clonogenic ability), senescence (β-galactosidase activity) and spread potential (invasion and migration assays using the Boyden chamber approach) in all these cells. The results showed that the DCM, EtOAc, and Hex extracts display a selective antitumoral effect in gastric cancer cells by affecting all the cancer parameters tested. These findings reveal an attractive antitumoral potential of <i>L. rivularis</i> flower extracts by targeting several acquired capabilities of cancer cells. |
| first_indexed | 2024-03-09T12:20:36Z |
| format | Article |
| id | doaj.art-2adaa76662c8419491a2ac72a68313f3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:20:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2adaa76662c8419491a2ac72a68313f32023-11-30T22:40:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242143910.3390/ijms24021439Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer CellsNicolás Carrasco0Maritza Garrido1Iván Montenegro2Alejandro Madrid3Ricardo Hartley4Iván González5Mariaignacia Rubilar6Joan Villena7Manuel Valenzuela-Valderrama8Laboratorio de Microbiología Celular, Instituto de Investigación y Postgrado, Universidad Central de Chile, Lord Cochrane 418, Santiago 8330546, ChileLaboratorio de Endocrinología y Biología de la Reproducción, Hospital Clínico Universidad de Chile, Santiago 8380456, ChileCentro de Investigaciones Biomédicas, Escuela de Obstetricia, Universidad de Valparaíso, Angamos 655, Reñaca, Viña del Mar 2340000, ChileLaboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Química, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, ChileLaboratorio de Microbiología Celular, Instituto de Investigación y Postgrado, Universidad Central de Chile, Lord Cochrane 418, Santiago 8330546, ChileDepartamento de Química, Facultad de Ciencias Naturales, Matemática y del Medio Ambiente, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago 7800003, ChileLaboratorio de Microbiología Celular, Instituto de Investigación y Postgrado, Universidad Central de Chile, Lord Cochrane 418, Santiago 8330546, ChileCentro de Investigaciones Biomédicas, Escuela de Medicina, Universidad de Valparaíso, Angamos 655, Reñaca, Viña del Mar 2340000, ChileLaboratorio de Microbiología Celular, Instituto de Investigación y Postgrado, Universidad Central de Chile, Lord Cochrane 418, Santiago 8330546, Chile<i>Leptocarpha rivularis</i> is a native South American plant used ancestrally by Mapuche people to treat gastrointestinal ailments. <i>L. rivularis</i> flower extracts are rich in molecules with therapeutic potential, including the sesquiterpene lactone leptocarpin, which displays cytotoxic effects against various cancer types in vitro. However, the combination of active molecules in these extracts could offer a hitherto unexplored potential for targeting cancer. In this study, we investigated the effect of <i>L. rivularis</i> flower extracts on the proliferation, survival, and spread parameters of gastric cancer cells in vitro. Gastric cancer (AGS and MKN-45) and normal immortalized (GES-1) cell lines were treated with different concentrations of <i>L. rivularis</i> flower extracts (DCM, Hex, EtOAc, and EtOH) and we determined the changes in proliferation (MTS assay, cell cycle analysis), cell viability/cytotoxicity (trypan blue exclusion assay, DEVDase activity, mitochondrial membrane potential MMP, and clonogenic ability), senescence (β-galactosidase activity) and spread potential (invasion and migration assays using the Boyden chamber approach) in all these cells. The results showed that the DCM, EtOAc, and Hex extracts display a selective antitumoral effect in gastric cancer cells by affecting all the cancer parameters tested. These findings reveal an attractive antitumoral potential of <i>L. rivularis</i> flower extracts by targeting several acquired capabilities of cancer cells.https://www.mdpi.com/1422-0067/24/2/1439gastric cancer<i>Leptocarpha rivularis</i> flowersleptocarpinorganic extractsinvasionmigration |
| spellingShingle | Nicolás Carrasco Maritza Garrido Iván Montenegro Alejandro Madrid Ricardo Hartley Iván González Mariaignacia Rubilar Joan Villena Manuel Valenzuela-Valderrama Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells International Journal of Molecular Sciences gastric cancer <i>Leptocarpha rivularis</i> flowers leptocarpin organic extracts invasion migration |
| title | Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells |
| title_full | Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells |
| title_fullStr | Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells |
| title_full_unstemmed | Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells |
| title_short | Antitumoral Activity of <i>Leptocarpha rivularis</i> Flower Extracts against Gastric Cancer Cells |
| title_sort | antitumoral activity of i leptocarpha rivularis i flower extracts against gastric cancer cells |
| topic | gastric cancer <i>Leptocarpha rivularis</i> flowers leptocarpin organic extracts invasion migration |
| url | https://www.mdpi.com/1422-0067/24/2/1439 |
| work_keys_str_mv | AT nicolascarrasco antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT maritzagarrido antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT ivanmontenegro antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT alejandromadrid antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT ricardohartley antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT ivangonzalez antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT mariaignaciarubilar antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT joanvillena antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells AT manuelvalenzuelavalderrama antitumoralactivityofileptocarpharivularisiflowerextractsagainstgastriccancercells |