Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins
Membrane proteins have evolved to work optimally within the complex environment of the biological membrane. Consequently, interactions with surrounding lipids are part of their molecular mechanism. Yet, the identification of lipid–protein interactions and the assessment of their molecular role is an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/14/7267 |
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| author | Léni Jodaitis Thomas van Oene Chloé Martens |
| author_facet | Léni Jodaitis Thomas van Oene Chloé Martens |
| author_sort | Léni Jodaitis |
| collection | DOAJ |
| description | Membrane proteins have evolved to work optimally within the complex environment of the biological membrane. Consequently, interactions with surrounding lipids are part of their molecular mechanism. Yet, the identification of lipid–protein interactions and the assessment of their molecular role is an experimental challenge. Recently, biophysical approaches have emerged that are compatible with the study of membrane proteins in an environment closer to the biological membrane. These novel approaches revealed specific mechanisms of regulation of membrane protein function. Lipids have been shown to play a role in oligomerization, conformational transitions or allosteric coupling. In this review, we summarize the recent biophysical approaches, or combination thereof, that allow to decipher the role of lipid–protein interactions in the mechanism of membrane proteins. |
| first_indexed | 2024-03-10T09:38:00Z |
| format | Article |
| id | doaj.art-2adae1f123014beb98bec5c479176dbd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:38:00Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2adae1f123014beb98bec5c479176dbd2023-11-22T03:55:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214726710.3390/ijms22147267Assessing the Role of Lipids in the Molecular Mechanism of Membrane ProteinsLéni Jodaitis0Thomas van Oene1Chloé Martens2Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, 1050 Brussels, BelgiumCenter for Structural Biology and Bioinformatics, Université Libre de Bruxelles, 1050 Brussels, BelgiumCenter for Structural Biology and Bioinformatics, Université Libre de Bruxelles, 1050 Brussels, BelgiumMembrane proteins have evolved to work optimally within the complex environment of the biological membrane. Consequently, interactions with surrounding lipids are part of their molecular mechanism. Yet, the identification of lipid–protein interactions and the assessment of their molecular role is an experimental challenge. Recently, biophysical approaches have emerged that are compatible with the study of membrane proteins in an environment closer to the biological membrane. These novel approaches revealed specific mechanisms of regulation of membrane protein function. Lipids have been shown to play a role in oligomerization, conformational transitions or allosteric coupling. In this review, we summarize the recent biophysical approaches, or combination thereof, that allow to decipher the role of lipid–protein interactions in the mechanism of membrane proteins.https://www.mdpi.com/1422-0067/22/14/7267membrane proteinlipid–protein interactioncryo-electron microscopyhydrogen–deuterium exchange mass spectrometrynative mass spectrometrysingle-molecule Förster resonance energy transfer |
| spellingShingle | Léni Jodaitis Thomas van Oene Chloé Martens Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins International Journal of Molecular Sciences membrane protein lipid–protein interaction cryo-electron microscopy hydrogen–deuterium exchange mass spectrometry native mass spectrometry single-molecule Förster resonance energy transfer |
| title | Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins |
| title_full | Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins |
| title_fullStr | Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins |
| title_full_unstemmed | Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins |
| title_short | Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins |
| title_sort | assessing the role of lipids in the molecular mechanism of membrane proteins |
| topic | membrane protein lipid–protein interaction cryo-electron microscopy hydrogen–deuterium exchange mass spectrometry native mass spectrometry single-molecule Förster resonance energy transfer |
| url | https://www.mdpi.com/1422-0067/22/14/7267 |
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