Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell
Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. De...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2020-12-01
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| Series: | Indonesian Journal of Tropical and Infectious Disease |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/IJTID/article/view/11776 |
| _version_ | 1818594758645252096 |
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| author | Teguh Hari Sucipto Aswandi Wibrianto Fahimah Martak Siti Churrotin Ilham Harlan Amarullah Harsasi Setyawati Puspa Wardhani Aryati Aryati Soegeng Soegijanto |
| author_facet | Teguh Hari Sucipto Aswandi Wibrianto Fahimah Martak Siti Churrotin Ilham Harlan Amarullah Harsasi Setyawati Puspa Wardhani Aryati Aryati Soegeng Soegijanto |
| author_sort | Teguh Hari Sucipto |
| collection | DOAJ |
| description | Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many efforts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 μg/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections |
| first_indexed | 2024-12-16T11:05:13Z |
| format | Article |
| id | doaj.art-2adcca9096ed4e1fa1d6a29102fe42bd |
| institution | Directory Open Access Journal |
| issn | 2085-1103 2356-0991 |
| language | English |
| last_indexed | 2024-12-16T11:05:13Z |
| publishDate | 2020-12-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Indonesian Journal of Tropical and Infectious Disease |
| spelling | doaj.art-2adcca9096ed4e1fa1d6a29102fe42bd2022-12-21T22:33:52ZengUniversitas AirlanggaIndonesian Journal of Tropical and Infectious Disease2085-11032356-09912020-12-018318318810.20473/ijtid.v8i3.117768749Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero CellTeguh Hari Sucipto0Aswandi Wibrianto1Fahimah Martak2Siti Churrotin3Ilham Harlan Amarullah4Harsasi Setyawati5Puspa Wardhani6Aryati Aryati7Soegeng Soegijanto8Dengue Study Group, Institute of Tropical DiseaseUndergraduate Student, Department of Chemistry, Faculty Science and Technology, Universitas AirlanggaDepartment of Chemistry, Faculty of Natural Science, Institut Teknologi Sepuluh NopemberDengue Study Group, Institute of Tropical DiseaseDengue Study Group, Institute of Tropical DiseaseDepartment of Chemistry, Universitas AirlanggaDengue Study Group, Institute of Tropical DiseaseDengue Study Group, Institute of Tropical DiseaseDengue Study Group, Institute of Tropical DiseaseDengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many efforts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 μg/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infectionshttps://e-journal.unair.ac.id/IJTID/article/view/11776zinc (ii), complex compound, cytotoxicity, inhibitory activity, denv-2 |
| spellingShingle | Teguh Hari Sucipto Aswandi Wibrianto Fahimah Martak Siti Churrotin Ilham Harlan Amarullah Harsasi Setyawati Puspa Wardhani Aryati Aryati Soegeng Soegijanto Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell Indonesian Journal of Tropical and Infectious Disease zinc (ii), complex compound, cytotoxicity, inhibitory activity, denv-2 |
| title | Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell |
| title_full | Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell |
| title_fullStr | Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell |
| title_full_unstemmed | Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell |
| title_short | Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell |
| title_sort | effect of zinc ii 2 4 5 triphenyl 1h imidazole complex against replication denv 2 in vero cell |
| topic | zinc (ii), complex compound, cytotoxicity, inhibitory activity, denv-2 |
| url | https://e-journal.unair.ac.id/IJTID/article/view/11776 |
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