Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone
Yiming Wang,1– 3 Xue Wang,1– 3 Yang Yang,1– 3 Qianghua Quan,1– 3 Tong Huo,1– 3 Simin Yang,4 Ruijun Ju,4 Quan An1– 3,5 1Research and Development Department, Yunnan Baiyao Group Health Products Co, Ltd, Kunming, People’s Republic of China; 2East Asia Skin Health Research Center, Beijing, People’s Repu...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-09-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
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| Online Access: | https://www.dovepress.com/comparison-of-the-in-vitro-anti-inflammatory-effect-of-cannabidiol-to--peer-reviewed-fulltext-article-CCID |
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| author | Wang Y Wang X Yang Y Quan Q Huo T Yang S Ju R An Q |
| author_facet | Wang Y Wang X Yang Y Quan Q Huo T Yang S Ju R An Q |
| author_sort | Wang Y |
| collection | DOAJ |
| description | Yiming Wang,1– 3 Xue Wang,1– 3 Yang Yang,1– 3 Qianghua Quan,1– 3 Tong Huo,1– 3 Simin Yang,4 Ruijun Ju,4 Quan An1– 3,5 1Research and Development Department, Yunnan Baiyao Group Health Products Co, Ltd, Kunming, People’s Republic of China; 2East Asia Skin Health Research Center, Beijing, People’s Republic of China; 3Research and Development Department, REAL DermaSci & Biotech Co, Ltd, Beijing, People’s Republic of China; 4Beijing Key Laboratory of Enze Biomass Fine Chemicals, Department of Pharmaceutical Engineering, Beijing Institute of Petrochemical Technology, Beijing, People’s Republic of China; 5Research and Development Department, Yunnan Baiyao Group Shanghai Science & Technology Co, Ltd, Shanghai, People’s Republic of ChinaCorrespondence: Quan An, Yunnan Baiyao Group Health Products Co, Ltd, 3686 Yunnan Baiyao Street Chenggong District, Kunming, 650500, People’s Republic of China, Tel +86-13529091004, Email ynbyanquan@sina.comBackground: Cannabidiol (CBD) is a non-psychoactive phytocannabinoid constituent of Cannabis sativa with pain-relieving and anti-inflammatory properties. With the emphasis on natural ingredients in cosmetics, CBD has become a new cosmetic ingredient due to its ability to alleviate inflammation. However, in-depth studies that directly compare the effective mechanism and the therapeutic potential of CBD are still needed.Purpose: The aim of the present study was to investigate the anti-inflammatory effect of CBD in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and compare it to dexamethasone (DEX).Methods: RAW264.7 macrophages in the logarithmic growth phase were incubated in the presence or absence of LPS. After that, the production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were measured. A luciferase reporter assay for nuclear factor kappa B (NF-κB) was performed, and the phosphorylation levels of the mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways were measured.Results: The present study indicated that CBD had a similar anti-inflammatory effect to DEX by attenuating the LPS-induced production of NO, IL-6, and TNF-α. However, only CBD attenuated JNK phosphorylation levels, and only DEX attenuated IKK phosphorylation levels.Conclusion: These results suggested that CBD and DEX exhibit similar anti-inflammatory effects on LPS-induced RAW264.7 macrophages mainly through suppressing the MAPK and NF-κB signaling pathways, but with different intracellular mechanisms. These findings suggested that CBD may be considered a natural anti-inflammatory agent for protecting skin from immune disorders.Graphical Abstract: Keywords: cannabidiol, dexamethasone, LPS, RAW264.7, anti-inflammation |
| first_indexed | 2024-04-14T08:25:52Z |
| format | Article |
| id | doaj.art-2addf1b4905c42518d4d7bd1d015e4fe |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2024-04-14T08:25:52Z |
| publishDate | 2022-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-2addf1b4905c42518d4d7bd1d015e4fe2022-12-22T02:04:04ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152022-09-01Volume 151959196778305Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to DexamethasoneWang YWang XYang YQuan QHuo TYang SJu RAn QYiming Wang,1– 3 Xue Wang,1– 3 Yang Yang,1– 3 Qianghua Quan,1– 3 Tong Huo,1– 3 Simin Yang,4 Ruijun Ju,4 Quan An1– 3,5 1Research and Development Department, Yunnan Baiyao Group Health Products Co, Ltd, Kunming, People’s Republic of China; 2East Asia Skin Health Research Center, Beijing, People’s Republic of China; 3Research and Development Department, REAL DermaSci & Biotech Co, Ltd, Beijing, People’s Republic of China; 4Beijing Key Laboratory of Enze Biomass Fine Chemicals, Department of Pharmaceutical Engineering, Beijing Institute of Petrochemical Technology, Beijing, People’s Republic of China; 5Research and Development Department, Yunnan Baiyao Group Shanghai Science & Technology Co, Ltd, Shanghai, People’s Republic of ChinaCorrespondence: Quan An, Yunnan Baiyao Group Health Products Co, Ltd, 3686 Yunnan Baiyao Street Chenggong District, Kunming, 650500, People’s Republic of China, Tel +86-13529091004, Email ynbyanquan@sina.comBackground: Cannabidiol (CBD) is a non-psychoactive phytocannabinoid constituent of Cannabis sativa with pain-relieving and anti-inflammatory properties. With the emphasis on natural ingredients in cosmetics, CBD has become a new cosmetic ingredient due to its ability to alleviate inflammation. However, in-depth studies that directly compare the effective mechanism and the therapeutic potential of CBD are still needed.Purpose: The aim of the present study was to investigate the anti-inflammatory effect of CBD in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and compare it to dexamethasone (DEX).Methods: RAW264.7 macrophages in the logarithmic growth phase were incubated in the presence or absence of LPS. After that, the production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were measured. A luciferase reporter assay for nuclear factor kappa B (NF-κB) was performed, and the phosphorylation levels of the mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways were measured.Results: The present study indicated that CBD had a similar anti-inflammatory effect to DEX by attenuating the LPS-induced production of NO, IL-6, and TNF-α. However, only CBD attenuated JNK phosphorylation levels, and only DEX attenuated IKK phosphorylation levels.Conclusion: These results suggested that CBD and DEX exhibit similar anti-inflammatory effects on LPS-induced RAW264.7 macrophages mainly through suppressing the MAPK and NF-κB signaling pathways, but with different intracellular mechanisms. These findings suggested that CBD may be considered a natural anti-inflammatory agent for protecting skin from immune disorders.Graphical Abstract: Keywords: cannabidiol, dexamethasone, LPS, RAW264.7, anti-inflammationhttps://www.dovepress.com/comparison-of-the-in-vitro-anti-inflammatory-effect-of-cannabidiol-to--peer-reviewed-fulltext-article-CCIDcannabidioldexamethasonelpsraw264.7anti-inflammation |
| spellingShingle | Wang Y Wang X Yang Y Quan Q Huo T Yang S Ju R An Q Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone Clinical, Cosmetic and Investigational Dermatology cannabidiol dexamethasone lps raw264.7 anti-inflammation |
| title | Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone |
| title_full | Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone |
| title_fullStr | Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone |
| title_full_unstemmed | Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone |
| title_short | Comparison of the in vitro Anti-Inflammatory Effect of Cannabidiol to Dexamethasone |
| title_sort | comparison of the in vitro anti inflammatory effect of cannabidiol to dexamethasone |
| topic | cannabidiol dexamethasone lps raw264.7 anti-inflammation |
| url | https://www.dovepress.com/comparison-of-the-in-vitro-anti-inflammatory-effect-of-cannabidiol-to--peer-reviewed-fulltext-article-CCID |
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