Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya
Background: The development of alternative control measures, such as phage therapy or adjunctive therapy, is urgently needed to manage the dissemination of carbapenemase-producing Klebsiella pneumoniae. Objective: This study aimed to evaluate the therapeutic potential of formulated phage cocktails...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AOSIS
2022-07-01
|
| Series: | African Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://ajlmonline.org/index.php/ajlm/article/view/1803 |
| _version_ | 1811315882437115904 |
|---|---|
| author | Noutin F. Michodigni Atunga Nyachieo Juliah K. Akhwale Gabriel Magoma Abdoul-Salam Ouédraogo Andrew N. Kimang’a |
| author_facet | Noutin F. Michodigni Atunga Nyachieo Juliah K. Akhwale Gabriel Magoma Abdoul-Salam Ouédraogo Andrew N. Kimang’a |
| author_sort | Noutin F. Michodigni |
| collection | DOAJ |
| description | Background: The development of alternative control measures, such as phage therapy or adjunctive therapy, is urgently needed to manage the dissemination of carbapenemase-producing Klebsiella pneumoniae.
Objective: This study aimed to evaluate the therapeutic potential of formulated phage cocktails and their interaction with select antibiotics in inhibiting the growth of carbapenemase-producing K. pneumoniae clinical isolate in vitro in Kenya.
Methods: The study was conducted from February 2021 to October 2021 at the Institute of Primate Research, Nairobi, Kenya. Phage cocktails were formulated based on the morphology and biological properties of precipitated Klebsiella phages. The efficacy of individual bacteriophages and phage cocktails as well as their combination with antibiotics were determined for their inhibitory activity on carbapenemase-producing K. pneumoniae (KP20).
Results: The precipitated bacteriophages were members of Myoviridae, Siphoviridae and Podoviridae. Regarding the evaluation of the phage cocktails, the absorbances at 600 nm of the bacterial culture treated with the two-phage cocktail (2φ MA) ranged from 0.173 to 0.246 at 16 h and 20 h whereas it peaked from 2.116 to 2.190 for the positive control. Moreover, the results of the adjunctive therapy showed that the optical density at 600 nm of the bacterial culture treated with 2φ MA was 0.186 at 24 h post-incubation time while it was 0.099 with the bacterial culture treated with imipenem in combination with 2φ MA.
Conclusion: This study demonstrated that the two-phage cocktail in combination with imipenem was able to synergistically delay the increase in carbapenemase-producing K. pneumoniae growth in vitro. |
| first_indexed | 2024-04-13T11:39:22Z |
| format | Article |
| id | doaj.art-2adff34a256845f88f1d92bd80e22459 |
| institution | Directory Open Access Journal |
| issn | 2225-2002 2225-2010 |
| language | English |
| last_indexed | 2024-04-13T11:39:22Z |
| publishDate | 2022-07-01 |
| publisher | AOSIS |
| record_format | Article |
| series | African Journal of Laboratory Medicine |
| spelling | doaj.art-2adff34a256845f88f1d92bd80e224592022-12-22T02:48:21ZengAOSISAfrican Journal of Laboratory Medicine2225-20022225-20102022-07-01111e1e810.4102/ajlm.v11i1.1803381Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in KenyaNoutin F. Michodigni0Atunga Nyachieo1Juliah K. Akhwale2Gabriel Magoma3Abdoul-Salam Ouédraogo4Andrew N. Kimang’a5Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences Technology and Innovation (PAUSTI), Nairobi, Kenya; and, Department of Reproductive Health and Biology, Institute of Primate Research (IPR), NairobiDepartment of Reproductive Health and Biology, Institute of Primate Research (IPR) NairobiDepartment of Zoology, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology (JKUAT), NairobiDepartment of Molecular Biology and Biotechnology Pan African University Institute for Basic Sciences Technology and Innovation (PAUSTI), Nairobi, Kenya; and, Department of Biochemistry, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology (JKUAT), NairobiDepartment of Medical Microbiology Laboratories, Souro-Sanou Teaching Hospital, Bobo-DioulassoDepartment of Medical Microbiology, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology (JKUAT), NairobiBackground: The development of alternative control measures, such as phage therapy or adjunctive therapy, is urgently needed to manage the dissemination of carbapenemase-producing Klebsiella pneumoniae. Objective: This study aimed to evaluate the therapeutic potential of formulated phage cocktails and their interaction with select antibiotics in inhibiting the growth of carbapenemase-producing K. pneumoniae clinical isolate in vitro in Kenya. Methods: The study was conducted from February 2021 to October 2021 at the Institute of Primate Research, Nairobi, Kenya. Phage cocktails were formulated based on the morphology and biological properties of precipitated Klebsiella phages. The efficacy of individual bacteriophages and phage cocktails as well as their combination with antibiotics were determined for their inhibitory activity on carbapenemase-producing K. pneumoniae (KP20). Results: The precipitated bacteriophages were members of Myoviridae, Siphoviridae and Podoviridae. Regarding the evaluation of the phage cocktails, the absorbances at 600 nm of the bacterial culture treated with the two-phage cocktail (2φ MA) ranged from 0.173 to 0.246 at 16 h and 20 h whereas it peaked from 2.116 to 2.190 for the positive control. Moreover, the results of the adjunctive therapy showed that the optical density at 600 nm of the bacterial culture treated with 2φ MA was 0.186 at 24 h post-incubation time while it was 0.099 with the bacterial culture treated with imipenem in combination with 2φ MA. Conclusion: This study demonstrated that the two-phage cocktail in combination with imipenem was able to synergistically delay the increase in carbapenemase-producing K. pneumoniae growth in vitro.https://ajlmonline.org/index.php/ajlm/article/view/1803phage cocktailantibioticsklebsiella pneumoniaecarbapenemaseabsorbances |
| spellingShingle | Noutin F. Michodigni Atunga Nyachieo Juliah K. Akhwale Gabriel Magoma Abdoul-Salam Ouédraogo Andrew N. Kimang’a Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya African Journal of Laboratory Medicine phage cocktail antibiotics klebsiella pneumoniae carbapenemase absorbances |
| title | Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya |
| title_full | Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya |
| title_fullStr | Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya |
| title_full_unstemmed | Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya |
| title_short | Formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase-producing Klebsiella pneumoniae in vitro in Kenya |
| title_sort | formulation of phage cocktails and evaluation of their interaction with antibiotics in inhibiting carbapenemase producing klebsiella pneumoniae in vitro in kenya |
| topic | phage cocktail antibiotics klebsiella pneumoniae carbapenemase absorbances |
| url | https://ajlmonline.org/index.php/ajlm/article/view/1803 |
| work_keys_str_mv | AT noutinfmichodigni formulationofphagecocktailsandevaluationoftheirinteractionwithantibioticsininhibitingcarbapenemaseproducingklebsiellapneumoniaeinvitroinkenya AT atunganyachieo formulationofphagecocktailsandevaluationoftheirinteractionwithantibioticsininhibitingcarbapenemaseproducingklebsiellapneumoniaeinvitroinkenya AT juliahkakhwale formulationofphagecocktailsandevaluationoftheirinteractionwithantibioticsininhibitingcarbapenemaseproducingklebsiellapneumoniaeinvitroinkenya AT gabrielmagoma formulationofphagecocktailsandevaluationoftheirinteractionwithantibioticsininhibitingcarbapenemaseproducingklebsiellapneumoniaeinvitroinkenya AT abdoulsalamouedraogo formulationofphagecocktailsandevaluationoftheirinteractionwithantibioticsininhibitingcarbapenemaseproducingklebsiellapneumoniaeinvitroinkenya AT andrewnkimanga formulationofphagecocktailsandevaluationoftheirinteractionwithantibioticsininhibitingcarbapenemaseproducingklebsiellapneumoniaeinvitroinkenya |