Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells
The most prevalent Ca<sup>2+</sup>-buffer proteins (CaBPs: parvalbumin—PV; calbindin—CaB; calretinin—CaR) are widely expressed by various neurons throughout the brain, including the retinal ganglion cells (RGCs). Even though their retinal expression has been...
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MDPI AG
2020-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/4/792 |
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| author | Tamás Kovács-Öller Gergely Szarka Ádám J. Tengölics Alma Ganczer Boglárka Balogh Edina Szabó-Meleg Miklós Nyitrai Béla Völgyi |
| author_facet | Tamás Kovács-Öller Gergely Szarka Ádám J. Tengölics Alma Ganczer Boglárka Balogh Edina Szabó-Meleg Miklós Nyitrai Béla Völgyi |
| author_sort | Tamás Kovács-Öller |
| collection | DOAJ |
| description | The most prevalent Ca<sup>2+</sup>-buffer proteins (CaBPs: parvalbumin—PV; calbindin—CaB; calretinin—CaR) are widely expressed by various neurons throughout the brain, including the retinal ganglion cells (RGCs). Even though their retinal expression has been extensively studied, a coherent assessment of topographical variations is missing. To examine this, we performed immunohistochemistry (IHC) in mouse retinas. We found variability in the expression levels and cell numbers for CaR, with stronger and more numerous labels in the dorso-central area. CaBP+ cells contributed to RGCs with all soma sizes, indicating heterogeneity. We separated four to nine RGC clusters in each area based on expression levels and soma sizes. Besides the overall high variety in cluster number and size, the peripheral half of the temporal retina showed the greatest cluster number, indicating a better separation of RGC subtypes there. Multiple labels showed that 39% of the RGCs showed positivity for a single CaBP, 30% expressed two CaBPs, 25% showed no CaBP expression, and 6% expressed all three proteins. Finally, we observed an inverse relation between CaB and CaR expression levels in CaB/CaR dual- and CaB/CaR/PV triple-labeled RGCs, suggesting a mutual complementary function. |
| first_indexed | 2024-03-12T09:44:52Z |
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| id | doaj.art-2ae3b31d10234367954676439cdce0b2 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T09:44:52Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2ae3b31d10234367954676439cdce0b22023-09-02T12:57:57ZengMDPI AGCells2073-44092020-03-019479210.3390/cells9040792cells9040792Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion CellsTamás Kovács-Öller0Gergely Szarka1Ádám J. Tengölics2Alma Ganczer3Boglárka Balogh4Edina Szabó-Meleg5Miklós Nyitrai6Béla Völgyi7János Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryJános Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryThe most prevalent Ca<sup>2+</sup>-buffer proteins (CaBPs: parvalbumin—PV; calbindin—CaB; calretinin—CaR) are widely expressed by various neurons throughout the brain, including the retinal ganglion cells (RGCs). Even though their retinal expression has been extensively studied, a coherent assessment of topographical variations is missing. To examine this, we performed immunohistochemistry (IHC) in mouse retinas. We found variability in the expression levels and cell numbers for CaR, with stronger and more numerous labels in the dorso-central area. CaBP+ cells contributed to RGCs with all soma sizes, indicating heterogeneity. We separated four to nine RGC clusters in each area based on expression levels and soma sizes. Besides the overall high variety in cluster number and size, the peripheral half of the temporal retina showed the greatest cluster number, indicating a better separation of RGC subtypes there. Multiple labels showed that 39% of the RGCs showed positivity for a single CaBP, 30% expressed two CaBPs, 25% showed no CaBP expression, and 6% expressed all three proteins. Finally, we observed an inverse relation between CaB and CaR expression levels in CaB/CaR dual- and CaB/CaR/PV triple-labeled RGCs, suggesting a mutual complementary function.https://www.mdpi.com/2073-4409/9/4/792parvalbumincalretinincalbindinexpressionretinatopographyganglion cellcalcium buffer proteinexpression |
| spellingShingle | Tamás Kovács-Öller Gergely Szarka Ádám J. Tengölics Alma Ganczer Boglárka Balogh Edina Szabó-Meleg Miklós Nyitrai Béla Völgyi Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells Cells parvalbumin calretinin calbindin expression retina topography ganglion cell calcium buffer protein expression |
| title | Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells |
| title_full | Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells |
| title_fullStr | Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells |
| title_full_unstemmed | Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells |
| title_short | Spatial Expression Pattern of the Major Ca<sup>2+</sup>-Buffer Proteins in Mouse Retinal Ganglion Cells |
| title_sort | spatial expression pattern of the major ca sup 2 sup buffer proteins in mouse retinal ganglion cells |
| topic | parvalbumin calretinin calbindin expression retina topography ganglion cell calcium buffer protein expression |
| url | https://www.mdpi.com/2073-4409/9/4/792 |
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