Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes
Background. Zinc is essential for male reproductive function as it is necessary for spermatozoa maturation, capacitation, acrosome reaction, and fertilization. It has been established that the use of chelates compounds of metals with amino acids, nucleotides, peptides, and carbohydrates is not only...
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| Format: | Article |
| Language: | English |
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Igor Sikorsky Kyiv Polytechnic Institute
2023-11-01
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| Series: | Innovative Biosystems and Bioengineering |
| Subjects: | |
| Online Access: | http://ibb.kpi.ua/article/view/284774 |
| _version_ | 1797356585778937856 |
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| author | Oksana Slyvchuk Oksana Shtapenko Iryna Yaremchuk Serhiy Kornyat Yevhen Dzen |
| author_facet | Oksana Slyvchuk Oksana Shtapenko Iryna Yaremchuk Serhiy Kornyat Yevhen Dzen |
| author_sort | Oksana Slyvchuk |
| collection | DOAJ |
| description | Background. Zinc is essential for male reproductive function as it is necessary for spermatozoa maturation, capacitation, acrosome reaction, and fertilization. It has been established that the use of chelates compounds of metals with amino acids, nucleotides, peptides, and carbohydrates is not only more effective but also economically justified.
Objective. The aim of this study was to evaluate and compare the effect of different concentrations of Zn glutamic amino acid chelate (ZnGlu) on boar spermatozoa motility, viability parameters, and prooxidant-antioxidant homeostasis during in vitro incubation.
Methods. Freshly ejaculated boar semen, after the addition of "Ecosperm" diluent, was divided into groups: a control group and three experimental groups. Zinc glutamate was supplemented to the experimental samples at concentrations of 1.0, 2.0, and 5.0 µg/ml, respectively. Semen samples were stored at 18 °C for 4 days. Every 24 hours, sperm motility and viability and the antioxidant status were assessed by the level of diene conjugates (DC), concentration of malondialdehydere (MDA), and the activities of catalase and superoxide dismutase.
Results. It was observed that boar sperm motility decreased with the extension of storage time, while the addition of 2.0 and 5.0 µg ZnGlu significantly improved sperm total motility and the percentage of vitality spermatozoa during 48–96 hours of incubation. Our studies also demonstrate that ZnGlu possess a protective effect in alleviating oxidative stress in boar sperm in vitro. The addition of ZnGlu significantly reduced the content of MDA and DC in ejaculate samples in all experimental groups during incubation, compared to the control group. Moreover, the activity of superoxide dismutase and catalase increased after adding ZnGlu to the boar semen, especially at concentrations of 2.0 and 5.0 mg/ml (P < 0.05).
Conclusions. These data demonstrate that the supplementation of zinc glutamate enhances the antioxidant defence system of sperm and improves quality of boar semen in vitro. |
| first_indexed | 2024-03-08T14:29:50Z |
| format | Article |
| id | doaj.art-2ae4ad6b9b9a42c49cc4c065f266f04b |
| institution | Directory Open Access Journal |
| issn | 2616-177X |
| language | English |
| last_indexed | 2024-03-08T14:29:50Z |
| publishDate | 2023-11-01 |
| publisher | Igor Sikorsky Kyiv Polytechnic Institute |
| record_format | Article |
| series | Innovative Biosystems and Bioengineering |
| spelling | doaj.art-2ae4ad6b9b9a42c49cc4c065f266f04b2024-01-12T12:17:15ZengIgor Sikorsky Kyiv Polytechnic InstituteInnovative Biosystems and Bioengineering2616-177X2023-11-017431110.20535/ibb.2023.7.4.284774322998Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant EnzymesOksana Slyvchuk0https://orcid.org/0000-0002-0229-5848Oksana Shtapenko1https://orcid.org/0000-0002-1192-8432Iryna Yaremchuk2https://orcid.org/0000-0001-8787-2132Serhiy Kornyat3https://orcid.org/0000-0002-1430-7754Yevhen Dzen4https://orcid.org/0000-0003-3095-9258Institute of Animal Biology NAASInstitute of Animal Biology of NAASInstitute of Animal Biology NAASInstitute of Animal Biology NAASInstitute of Animal Biology NAASBackground. Zinc is essential for male reproductive function as it is necessary for spermatozoa maturation, capacitation, acrosome reaction, and fertilization. It has been established that the use of chelates compounds of metals with amino acids, nucleotides, peptides, and carbohydrates is not only more effective but also economically justified. Objective. The aim of this study was to evaluate and compare the effect of different concentrations of Zn glutamic amino acid chelate (ZnGlu) on boar spermatozoa motility, viability parameters, and prooxidant-antioxidant homeostasis during in vitro incubation. Methods. Freshly ejaculated boar semen, after the addition of "Ecosperm" diluent, was divided into groups: a control group and three experimental groups. Zinc glutamate was supplemented to the experimental samples at concentrations of 1.0, 2.0, and 5.0 µg/ml, respectively. Semen samples were stored at 18 °C for 4 days. Every 24 hours, sperm motility and viability and the antioxidant status were assessed by the level of diene conjugates (DC), concentration of malondialdehydere (MDA), and the activities of catalase and superoxide dismutase. Results. It was observed that boar sperm motility decreased with the extension of storage time, while the addition of 2.0 and 5.0 µg ZnGlu significantly improved sperm total motility and the percentage of vitality spermatozoa during 48–96 hours of incubation. Our studies also demonstrate that ZnGlu possess a protective effect in alleviating oxidative stress in boar sperm in vitro. The addition of ZnGlu significantly reduced the content of MDA and DC in ejaculate samples in all experimental groups during incubation, compared to the control group. Moreover, the activity of superoxide dismutase and catalase increased after adding ZnGlu to the boar semen, especially at concentrations of 2.0 and 5.0 mg/ml (P < 0.05). Conclusions. These data demonstrate that the supplementation of zinc glutamate enhances the antioxidant defence system of sperm and improves quality of boar semen in vitro.http://ibb.kpi.ua/article/view/284774sperm qualitysperm motilityzincboar semenantioxidants |
| spellingShingle | Oksana Slyvchuk Oksana Shtapenko Iryna Yaremchuk Serhiy Kornyat Yevhen Dzen Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes Innovative Biosystems and Bioengineering sperm quality sperm motility zinc boar semen antioxidants |
| title | Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes |
| title_full | Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes |
| title_fullStr | Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes |
| title_full_unstemmed | Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes |
| title_short | Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes |
| title_sort | effect of zinc supplementation to diluent boar semen on sperm characteristics and activity of antioxidant enzymes |
| topic | sperm quality sperm motility zinc boar semen antioxidants |
| url | http://ibb.kpi.ua/article/view/284774 |
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