Detection of <i>Candida albicans</i> Using a Manufactured Electrochemical Sensor

<i>Candida albicans</i> is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by...

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Bibliographic Details
Main Authors: Prakhar Dutta, Yi-Jung Lu, Han-Yu Hsieh, Tyng-Yuh Lee, Yi-Tzu Lee, Chao-Min Cheng, Yu-Jui Fan
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/12/2/166
Description
Summary:<i>Candida albicans</i> is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by devising a method for detecting <i>C. albicans</i> with the use of electrochemical sensors. Through this experiment, we propose a method for the detection of <i>C. albicans</i> in the system through the use of personal glucose meters. The hemicellulase was used to break down the cell wall of <i>C. albicans</i> to glucose and oligo, which can be detected by a glucose meter. The spiked samples were prepared suspending <i>C. albicans</i> in urine and serum, demonstrating the feasibility of the developed method in a real situation.
ISSN:2072-666X