Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh
Infections by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) are continuously expanding within the community. Chicken meat is usually contaminated by MRSA, and this contaminated chicken meat is an important source of foodborne infections in humans. In this study, a cross-secti...
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MDPI AG
2021-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/3/636 |
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| author | Mst. Sonia Parvin Md. Yamin Ali Sudipta Talukder Azimun Nahar Emdadul Haque Chowdhury Md. Tanvir Rahman Md. Taohidul Islam |
| author_facet | Mst. Sonia Parvin Md. Yamin Ali Sudipta Talukder Azimun Nahar Emdadul Haque Chowdhury Md. Tanvir Rahman Md. Taohidul Islam |
| author_sort | Mst. Sonia Parvin |
| collection | DOAJ |
| description | Infections by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) are continuously expanding within the community. Chicken meat is usually contaminated by MRSA, and this contaminated chicken meat is an important source of foodborne infections in humans. In this study, a cross-sectional supershop survey was conducted to determine the prevalence and antimicrobial resistance pattern of MRSA in 113 domestic frozen chicken meat samples purchased from nine branded supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of methicillin resistance gene in MRSA isolates. <i>S. aureus</i> was identified by standard culture-based and molecular methods, and subjected to antimicrobial susceptibility testing. MRSA was screened by cefoxitin disk diffusion test. Methicillin resistance gene was identified by PCR. Of samples, 54.9% were positive for <i>S. aureus</i>, and, of these, 37.1% isolates were identified as MRSA. All the isolates were multidrug resistant (MDR): 52.2% were resistant to 6–8 antimicrobial classes, and 47.8% isolates to 9–12 classes. Three (3.2%) isolates of <i>S. aureus</i> were possible extensively drug resistant. The highest rates of resistance were observed against cefoxitin (100%), followed by nalidixic acid, ampicillin and oxacillin (97.7%), colistin (91.3%), amoxicillin-clavulanic acid and amoxicillin (87%), penicillin-G and cloxacillin (82.6%), oxytetracycline (78.3%), and cefixime (73.9%). Screening of methicillin resistance gene revealed that 43.5% isolates of MRSA were positive for <i>mecA</i> gene. The high prevalence of MDR MRSA in frozen chicken meat samples in this study emphasizes the need for better sanitary education of food handlers in hygienic practices focusing on their potential role as reservoirs and spreaders of MRSA. |
| first_indexed | 2024-03-10T13:06:32Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:06:32Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-2ae9e956e6aa42118f67af42c65f87f72023-11-21T11:03:09ZengMDPI AGMicroorganisms2076-26072021-03-019363610.3390/microorganisms9030636Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in BangladeshMst. Sonia Parvin0Md. Yamin Ali1Sudipta Talukder2Azimun Nahar3Emdadul Haque Chowdhury4Md. Tanvir Rahman5Md. Taohidul Islam6Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshInfections by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) are continuously expanding within the community. Chicken meat is usually contaminated by MRSA, and this contaminated chicken meat is an important source of foodborne infections in humans. In this study, a cross-sectional supershop survey was conducted to determine the prevalence and antimicrobial resistance pattern of MRSA in 113 domestic frozen chicken meat samples purchased from nine branded supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of methicillin resistance gene in MRSA isolates. <i>S. aureus</i> was identified by standard culture-based and molecular methods, and subjected to antimicrobial susceptibility testing. MRSA was screened by cefoxitin disk diffusion test. Methicillin resistance gene was identified by PCR. Of samples, 54.9% were positive for <i>S. aureus</i>, and, of these, 37.1% isolates were identified as MRSA. All the isolates were multidrug resistant (MDR): 52.2% were resistant to 6–8 antimicrobial classes, and 47.8% isolates to 9–12 classes. Three (3.2%) isolates of <i>S. aureus</i> were possible extensively drug resistant. The highest rates of resistance were observed against cefoxitin (100%), followed by nalidixic acid, ampicillin and oxacillin (97.7%), colistin (91.3%), amoxicillin-clavulanic acid and amoxicillin (87%), penicillin-G and cloxacillin (82.6%), oxytetracycline (78.3%), and cefixime (73.9%). Screening of methicillin resistance gene revealed that 43.5% isolates of MRSA were positive for <i>mecA</i> gene. The high prevalence of MDR MRSA in frozen chicken meat samples in this study emphasizes the need for better sanitary education of food handlers in hygienic practices focusing on their potential role as reservoirs and spreaders of MRSA.https://www.mdpi.com/2076-2607/9/3/636methicillin-resistant <i>Staphylococcus aureus</i>multidrug resistance<i>mecA</i> genefrozen chicken meatBangladesh |
| spellingShingle | Mst. Sonia Parvin Md. Yamin Ali Sudipta Talukder Azimun Nahar Emdadul Haque Chowdhury Md. Tanvir Rahman Md. Taohidul Islam Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh Microorganisms methicillin-resistant <i>Staphylococcus aureus</i> multidrug resistance <i>mecA</i> gene frozen chicken meat Bangladesh |
| title | Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_full | Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_fullStr | Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_full_unstemmed | Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_short | Prevalence and Multidrug Resistance Pattern of Methicillin Resistant <i>S. aureus</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_sort | prevalence and multidrug resistance pattern of methicillin resistant i s aureus i isolated from frozen chicken meat in bangladesh |
| topic | methicillin-resistant <i>Staphylococcus aureus</i> multidrug resistance <i>mecA</i> gene frozen chicken meat Bangladesh |
| url | https://www.mdpi.com/2076-2607/9/3/636 |
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