Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition
Increasing rapeseed yield has always been a primary goal of rapeseed research and breeding. However, flowering time is a prerequisite for stable rapeseed yield and determines its adaptability to ecological regions. MIKC-type MADS-box (MICK) genes are a class of transcription factors that are involve...
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2022-04-01
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| author | Enqiang Zhou Yin Zhang Huadong Wang Zhibo Jia Xuejun Wang Jing Wen Jinxiong Shen Tingdong Fu Bin Yi |
| author_facet | Enqiang Zhou Yin Zhang Huadong Wang Zhibo Jia Xuejun Wang Jing Wen Jinxiong Shen Tingdong Fu Bin Yi |
| author_sort | Enqiang Zhou |
| collection | DOAJ |
| description | Increasing rapeseed yield has always been a primary goal of rapeseed research and breeding. However, flowering time is a prerequisite for stable rapeseed yield and determines its adaptability to ecological regions. MIKC-type MADS-box (MICK) genes are a class of transcription factors that are involved in various physiological and developmental processes in plants. To understand their role in floral transition-related pathways, a genome-wide screening was conducted with <i>Brassica napus</i> (<i>B. napus</i>), which revealed 172 members. Using previous data from a genome-wide association analysis of flowering traits, <i>BnaSVP</i> and <i>BnaSEP1</i> were identified as candidate flowering genes. Therefore, we used the CRISPR/Cas9 system to verify the function of <i>BnaSVP</i> and <i>BnaSEP1</i> in <i>B. napus</i>. T0 plants were edited efficiently at the <i>BnaSVP</i> and <i>BnaSEP1</i> target sites to generate homozygous and heterozygous mutants with most mutations stably inherited by the next generation. Notably, the mutant only showed the early flowering phenotype when all homologous copies of <i>BnaSVP</i> were edited, indicating functional redundancy between homologous copies. However, no changes in flowering were observed in the <i>BnaSEP1</i> mutant. Quantitative analysis of the pathway-related genes in the <i>BnaSVP</i> mutant revealed the upregulation of <i>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1</i> (<i>SOC1</i>) and <i>FLOWERING LOCUS T</i> (<i>FT</i>) genes, which promoted early flowering in the mutant. In summary, our study created early flowering mutants, which provided valuable resources for early maturing breeding, and provided a new method for improving polyploid crops. |
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| spelling | doaj.art-2aeb6732a4eb44758699b75f296037232023-12-01T21:03:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238428910.3390/ijms23084289Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral TransitionEnqiang Zhou0Yin Zhang1Huadong Wang2Zhibo Jia3Xuejun Wang4Jing Wen5Jinxiong Shen6Tingdong Fu7Bin Yi8National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaJiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226001, ChinaNational Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaJiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226001, ChinaNational Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaIncreasing rapeseed yield has always been a primary goal of rapeseed research and breeding. However, flowering time is a prerequisite for stable rapeseed yield and determines its adaptability to ecological regions. MIKC-type MADS-box (MICK) genes are a class of transcription factors that are involved in various physiological and developmental processes in plants. To understand their role in floral transition-related pathways, a genome-wide screening was conducted with <i>Brassica napus</i> (<i>B. napus</i>), which revealed 172 members. Using previous data from a genome-wide association analysis of flowering traits, <i>BnaSVP</i> and <i>BnaSEP1</i> were identified as candidate flowering genes. Therefore, we used the CRISPR/Cas9 system to verify the function of <i>BnaSVP</i> and <i>BnaSEP1</i> in <i>B. napus</i>. T0 plants were edited efficiently at the <i>BnaSVP</i> and <i>BnaSEP1</i> target sites to generate homozygous and heterozygous mutants with most mutations stably inherited by the next generation. Notably, the mutant only showed the early flowering phenotype when all homologous copies of <i>BnaSVP</i> were edited, indicating functional redundancy between homologous copies. However, no changes in flowering were observed in the <i>BnaSEP1</i> mutant. Quantitative analysis of the pathway-related genes in the <i>BnaSVP</i> mutant revealed the upregulation of <i>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1</i> (<i>SOC1</i>) and <i>FLOWERING LOCUS T</i> (<i>FT</i>) genes, which promoted early flowering in the mutant. In summary, our study created early flowering mutants, which provided valuable resources for early maturing breeding, and provided a new method for improving polyploid crops.https://www.mdpi.com/1422-0067/23/8/4289<i>Brassica napus</i>CRISPR/Cas9MIKC-type MADS-boxflowering<i>BnaSVP</i><i>BnaSEP1</i> |
| spellingShingle | Enqiang Zhou Yin Zhang Huadong Wang Zhibo Jia Xuejun Wang Jing Wen Jinxiong Shen Tingdong Fu Bin Yi Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition International Journal of Molecular Sciences <i>Brassica napus</i> CRISPR/Cas9 MIKC-type MADS-box flowering <i>BnaSVP</i> <i>BnaSEP1</i> |
| title | Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition |
| title_full | Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition |
| title_fullStr | Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition |
| title_full_unstemmed | Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition |
| title_short | Identification and Characterization of the MIKC-Type MADS-Box Gene Family in <i>Brassica napus</i> and Its Role in Floral Transition |
| title_sort | identification and characterization of the mikc type mads box gene family in i brassica napus i and its role in floral transition |
| topic | <i>Brassica napus</i> CRISPR/Cas9 MIKC-type MADS-box flowering <i>BnaSVP</i> <i>BnaSEP1</i> |
| url | https://www.mdpi.com/1422-0067/23/8/4289 |
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