Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq
Skeletal muscle, accounting for approximately 50% of body weight, is the largest and most important tissue. In this study, the gene expression profiles and pathways in skeletal muscle of Pekin duck were investigated and compared at embryonic day 17, 21, and 27 and postnatally at 6 months of age. An...
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MDPI AG
2021-03-01
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| author | Zhigang Hu Junting Cao Liyan Ge Jianqin Zhang Huilin Zhang Xiaolin Liu |
| author_facet | Zhigang Hu Junting Cao Liyan Ge Jianqin Zhang Huilin Zhang Xiaolin Liu |
| author_sort | Zhigang Hu |
| collection | DOAJ |
| description | Skeletal muscle, accounting for approximately 50% of body weight, is the largest and most important tissue. In this study, the gene expression profiles and pathways in skeletal muscle of Pekin duck were investigated and compared at embryonic day 17, 21, and 27 and postnatally at 6 months of age. An average of 49,555,936 reads in each sample was obtained from the transcriptome libraries. Over 70.0% of alternative splicing (AS) in each sample was mainly alternative 5′ first exon (transcription start site)—the first exon splicing (TSS) and alternative 3′ last exon (transcription terminal site)—the last exon splicing (TTS), indicating that TSS and TTS were the most common AS event in Pekin ducks, and these AS events were closely related to the regulation of muscle development at different growth stages. The results provided a valuable genomic resource for selective breeding and functional studies of genes. A total of 299 novel genes with ≥2 exons were obtained. There were 294 to 2806 differentially expressed genes (DEGs) in each pairwise comparison of Pekin duck. Notably, 90 DEGs in breast muscle and 9 DEGs in leg muscle were co-expressed at all developmental points. DEGs were validated by qPCR analysis, which confirmed the tendency of the expression. DEGs related to muscle development were involved in biological processes such as “endodermal cell differentiation”, “muscle cell cellular homeostasis”, “skeletal muscle tissue growth” and “skeletal muscle cell differentiation”, and were involved in pathways such as oxidative phosphorylation, ECM-receptor (extracellular matrix receptor) interaction, focal adhesion, carbon metabolism, and biosynthesis of amino acids. Some DEGs, including <i>MYL</i>4, <i>IGF2BP1</i>, <i>CSRP3</i>, <i>SPP1</i> and <i>KLHL31</i>, as well as <i>LAMB2</i>, <i>LAMA2</i>, <i>ITGB1</i> and <i>OPN</i>, played crucial roles in muscle growth and development. This study provides valuable information about the expression profile of mRNAs and pathways from duck skeletal muscle at different growth stages, and further functional study of these mRNAs and pathways could provide new ideas for studying the molecular networks of growth and development in duck skeletal muscle. |
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| language | English |
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| spelling | doaj.art-2aed6b0c3fd3443abd8efb2f870647fb2023-11-21T10:39:38ZengMDPI AGAnimals2076-26152021-03-0111383410.3390/ani11030834Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-SeqZhigang Hu0Junting Cao1Liyan Ge2Jianqin Zhang3Huilin Zhang4Xiaolin Liu5College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaSkeletal muscle, accounting for approximately 50% of body weight, is the largest and most important tissue. In this study, the gene expression profiles and pathways in skeletal muscle of Pekin duck were investigated and compared at embryonic day 17, 21, and 27 and postnatally at 6 months of age. An average of 49,555,936 reads in each sample was obtained from the transcriptome libraries. Over 70.0% of alternative splicing (AS) in each sample was mainly alternative 5′ first exon (transcription start site)—the first exon splicing (TSS) and alternative 3′ last exon (transcription terminal site)—the last exon splicing (TTS), indicating that TSS and TTS were the most common AS event in Pekin ducks, and these AS events were closely related to the regulation of muscle development at different growth stages. The results provided a valuable genomic resource for selective breeding and functional studies of genes. A total of 299 novel genes with ≥2 exons were obtained. There were 294 to 2806 differentially expressed genes (DEGs) in each pairwise comparison of Pekin duck. Notably, 90 DEGs in breast muscle and 9 DEGs in leg muscle were co-expressed at all developmental points. DEGs were validated by qPCR analysis, which confirmed the tendency of the expression. DEGs related to muscle development were involved in biological processes such as “endodermal cell differentiation”, “muscle cell cellular homeostasis”, “skeletal muscle tissue growth” and “skeletal muscle cell differentiation”, and were involved in pathways such as oxidative phosphorylation, ECM-receptor (extracellular matrix receptor) interaction, focal adhesion, carbon metabolism, and biosynthesis of amino acids. Some DEGs, including <i>MYL</i>4, <i>IGF2BP1</i>, <i>CSRP3</i>, <i>SPP1</i> and <i>KLHL31</i>, as well as <i>LAMB2</i>, <i>LAMA2</i>, <i>ITGB1</i> and <i>OPN</i>, played crucial roles in muscle growth and development. This study provides valuable information about the expression profile of mRNAs and pathways from duck skeletal muscle at different growth stages, and further functional study of these mRNAs and pathways could provide new ideas for studying the molecular networks of growth and development in duck skeletal muscle.https://www.mdpi.com/2076-2615/11/3/834skeletal muscletranscriptomeDEGpathwayPekin duck |
| spellingShingle | Zhigang Hu Junting Cao Liyan Ge Jianqin Zhang Huilin Zhang Xiaolin Liu Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq Animals skeletal muscle transcriptome DEG pathway Pekin duck |
| title | Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq |
| title_full | Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq |
| title_fullStr | Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq |
| title_full_unstemmed | Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq |
| title_short | Characterization and Comparative Transcriptomic Analysis of Skeletal Muscle in Pekin Duck at Different Growth Stages Using RNA-Seq |
| title_sort | characterization and comparative transcriptomic analysis of skeletal muscle in pekin duck at different growth stages using rna seq |
| topic | skeletal muscle transcriptome DEG pathway Pekin duck |
| url | https://www.mdpi.com/2076-2615/11/3/834 |
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