Gymnemic acids inhibit hyphal growth and virulence in Candida albicans.
Candida albicans is an opportunistic and polymorphic fungal pathogen that causes mucosal, disseminated and invasive infections in humans. Transition from the yeast form to the hyphal form is one of the key virulence factors in C. albicans contributing to macrophage evasion, tissue invasion and biofi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3770570?pdf=render |
| _version_ | 1818127933532798976 |
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| author | Govindsamy Vediyappan Vincent Dumontet Franck Pelissier Christophe d'Enfert |
| author_facet | Govindsamy Vediyappan Vincent Dumontet Franck Pelissier Christophe d'Enfert |
| author_sort | Govindsamy Vediyappan |
| collection | DOAJ |
| description | Candida albicans is an opportunistic and polymorphic fungal pathogen that causes mucosal, disseminated and invasive infections in humans. Transition from the yeast form to the hyphal form is one of the key virulence factors in C. albicans contributing to macrophage evasion, tissue invasion and biofilm formation. Nontoxic small molecules that inhibit C. albicans yeast-to-hypha conversion and hyphal growth could represent a valuable source for understanding pathogenic fungal morphogenesis, identifying drug targets and serving as templates for the development of novel antifungal agents. Here, we have identified the triterpenoid saponin family of gymnemic acids (GAs) as inhibitor of C. albicans morphogenesis. GAs were isolated and purified from Gymnema sylvestre leaves, the Ayurvedic traditional medicinal plant used to treat diabetes. Purified GAs had no effect on the growth and viability of C. albicans yeast cells but inhibited its yeast-to-hypha conversion under several hypha-inducing conditions, including the presence of serum. Moreover, GAs promoted the conversion of C. albicans hyphae into yeast cells under hypha inducing conditions. They also inhibited conidial germination and hyphal growth of Aspergillus sp. Finally, GAs inhibited the formation of invasive hyphae from C. albicans-infected Caenorhabditis elegans worms and rescued them from killing by C. albicans. Hence, GAs could be useful for various antifungal applications due to their traditional use in herbal medicine. |
| first_indexed | 2024-12-11T07:25:14Z |
| format | Article |
| id | doaj.art-2af081b7af2a4947836e31074e09e9cd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T07:25:14Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2af081b7af2a4947836e31074e09e9cd2022-12-22T01:15:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7418910.1371/journal.pone.0074189Gymnemic acids inhibit hyphal growth and virulence in Candida albicans.Govindsamy VediyappanVincent DumontetFranck PelissierChristophe d'EnfertCandida albicans is an opportunistic and polymorphic fungal pathogen that causes mucosal, disseminated and invasive infections in humans. Transition from the yeast form to the hyphal form is one of the key virulence factors in C. albicans contributing to macrophage evasion, tissue invasion and biofilm formation. Nontoxic small molecules that inhibit C. albicans yeast-to-hypha conversion and hyphal growth could represent a valuable source for understanding pathogenic fungal morphogenesis, identifying drug targets and serving as templates for the development of novel antifungal agents. Here, we have identified the triterpenoid saponin family of gymnemic acids (GAs) as inhibitor of C. albicans morphogenesis. GAs were isolated and purified from Gymnema sylvestre leaves, the Ayurvedic traditional medicinal plant used to treat diabetes. Purified GAs had no effect on the growth and viability of C. albicans yeast cells but inhibited its yeast-to-hypha conversion under several hypha-inducing conditions, including the presence of serum. Moreover, GAs promoted the conversion of C. albicans hyphae into yeast cells under hypha inducing conditions. They also inhibited conidial germination and hyphal growth of Aspergillus sp. Finally, GAs inhibited the formation of invasive hyphae from C. albicans-infected Caenorhabditis elegans worms and rescued them from killing by C. albicans. Hence, GAs could be useful for various antifungal applications due to their traditional use in herbal medicine.http://europepmc.org/articles/PMC3770570?pdf=render |
| spellingShingle | Govindsamy Vediyappan Vincent Dumontet Franck Pelissier Christophe d'Enfert Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. PLoS ONE |
| title | Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. |
| title_full | Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. |
| title_fullStr | Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. |
| title_full_unstemmed | Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. |
| title_short | Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. |
| title_sort | gymnemic acids inhibit hyphal growth and virulence in candida albicans |
| url | http://europepmc.org/articles/PMC3770570?pdf=render |
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