Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics
Gut microbiome plays an essential role in host health, and there is interest in utilizing diet to modulate the composition and function of microbial communities. Copra meal hydrolysate (CMH) is commonly used as a natural additive to enhance health. However, the gut microbiome is largely unknown at s...
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MDPI AG
2022-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/1/21 |
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| author | Amornthep Kingkaw Nachon Raethong Preecha Patumcharoenpol Narissara Suratannon Massalin Nakphaichit Suttipun Keawsompong Sittiruk Roytrakul Wanwipa Vongsangnak |
| author_facet | Amornthep Kingkaw Nachon Raethong Preecha Patumcharoenpol Narissara Suratannon Massalin Nakphaichit Suttipun Keawsompong Sittiruk Roytrakul Wanwipa Vongsangnak |
| author_sort | Amornthep Kingkaw |
| collection | DOAJ |
| description | Gut microbiome plays an essential role in host health, and there is interest in utilizing diet to modulate the composition and function of microbial communities. Copra meal hydrolysate (CMH) is commonly used as a natural additive to enhance health. However, the gut microbiome is largely unknown at species level and is associated with metabolic routes involving short-chain fatty acids (SCFAs). In this study, we aimed to analyze, using integrative metagenomics, the predominant species and metabolic routes involved in SCFAs production in the human gut microbiome after treatment with CMH. The effect of CMH treatment on the Thai gut microbiome was demonstrated using 16S rRNA genes with whole-metagenome shotgun (WMGS) sequencing technology. Accordingly, these results revealed that CMH has potentially beneficial effects on the gut microbiome. Twelve predominant bacterial species, as well as their potential metabolic routes, were involved in cooperative microbiome networks under sugar utilization (e.g., glucose, mannose, or xylose) and energy supply (e.g., NADH and ATP) in relation to SCFAs biosynthesis. These findings suggest that CMH may be used as a potential prebiotic diet for modulating and maintaining the gut microbiome. To our knowledge, this is the first study to reveal the predominant bacterial species and metabolic routes in the Thai gut microbiome after treatment with potential prebiotics. |
| first_indexed | 2024-03-09T13:31:55Z |
| format | Article |
| id | doaj.art-2af43735bfba4204b6e36e9a5568c516 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T13:31:55Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-2af43735bfba4204b6e36e9a5568c5162023-11-30T21:16:13ZengMDPI AGBiology2079-77372022-12-011212110.3390/biology12010021Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative MetagenomicsAmornthep Kingkaw0Nachon Raethong1Preecha Patumcharoenpol2Narissara Suratannon3Massalin Nakphaichit4Suttipun Keawsompong5Sittiruk Roytrakul6Wanwipa Vongsangnak7Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandInstitute of Nutrition, Mahidol University, Nakhon Pathom 73170, ThailandInterdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandCenter of Excellence for Allergy and Clinical Immunology, Division of Allergy, Immunology and Rheumatology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 144 Thailand Science Park, Phaholyothin Road, Pathum Thani 12120, ThailandDepartment of Zoology, Faculty of Sciences, Kasetsart University, Bangkok 10900, ThailandGut microbiome plays an essential role in host health, and there is interest in utilizing diet to modulate the composition and function of microbial communities. Copra meal hydrolysate (CMH) is commonly used as a natural additive to enhance health. However, the gut microbiome is largely unknown at species level and is associated with metabolic routes involving short-chain fatty acids (SCFAs). In this study, we aimed to analyze, using integrative metagenomics, the predominant species and metabolic routes involved in SCFAs production in the human gut microbiome after treatment with CMH. The effect of CMH treatment on the Thai gut microbiome was demonstrated using 16S rRNA genes with whole-metagenome shotgun (WMGS) sequencing technology. Accordingly, these results revealed that CMH has potentially beneficial effects on the gut microbiome. Twelve predominant bacterial species, as well as their potential metabolic routes, were involved in cooperative microbiome networks under sugar utilization (e.g., glucose, mannose, or xylose) and energy supply (e.g., NADH and ATP) in relation to SCFAs biosynthesis. These findings suggest that CMH may be used as a potential prebiotic diet for modulating and maintaining the gut microbiome. To our knowledge, this is the first study to reveal the predominant bacterial species and metabolic routes in the Thai gut microbiome after treatment with potential prebiotics.https://www.mdpi.com/2079-7737/12/1/21copra meal hydrolysatehuman gut microbiomemetagenomicsmetabolismshort-chain fatty acids |
| spellingShingle | Amornthep Kingkaw Nachon Raethong Preecha Patumcharoenpol Narissara Suratannon Massalin Nakphaichit Suttipun Keawsompong Sittiruk Roytrakul Wanwipa Vongsangnak Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics Biology copra meal hydrolysate human gut microbiome metagenomics metabolism short-chain fatty acids |
| title | Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics |
| title_full | Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics |
| title_fullStr | Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics |
| title_full_unstemmed | Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics |
| title_short | Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics |
| title_sort | analyzing predominant bacterial species and potential short chain fatty acid associated metabolic routes in human gut microbiome using integrative metagenomics |
| topic | copra meal hydrolysate human gut microbiome metagenomics metabolism short-chain fatty acids |
| url | https://www.mdpi.com/2079-7737/12/1/21 |
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