Emergent cooperation in microbial metabolism
Mixed microbial communities exhibit emergent biochemical properties not found in clonal monocultures. We report a new type of synthetic genetic interaction, synthetic mutualism in trans (SMIT), in which certain pairs of auxotrophic Escherichia coli mutants complement one another's growth by cro...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2010-01-01
|
| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2010.66 |
| _version_ | 1797281800363442176 |
|---|---|
| author | Edwin H Wintermute Pamela A Silver |
| author_facet | Edwin H Wintermute Pamela A Silver |
| author_sort | Edwin H Wintermute |
| collection | DOAJ |
| description | Mixed microbial communities exhibit emergent biochemical properties not found in clonal monocultures. We report a new type of synthetic genetic interaction, synthetic mutualism in trans (SMIT), in which certain pairs of auxotrophic Escherichia coli mutants complement one another's growth by cross‐feeding essential metabolites. We find significant metabolic synergy in 17% of 1035 such pairs tested, with SMIT partners identified throughout the metabolic network. Cooperative phenotypes show more growth on average by aiding the proliferation of their conjugate partner, thereby expanding the source of their own essential metabolites. We construct a quantitative, predictive, framework for describing SMIT interactions as governed by stoichiometric models of the metabolic networks of the interacting strains. |
| first_indexed | 2024-03-07T17:02:40Z |
| format | Article |
| id | doaj.art-2af4890edb3c401799b1fb70ee21be61 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T17:02:40Z |
| publishDate | 2010-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-2af4890edb3c401799b1fb70ee21be612024-03-03T03:11:51ZengSpringer NatureMolecular Systems Biology1744-42922010-01-0161n/an/a10.1038/msb.2010.66Emergent cooperation in microbial metabolismEdwin H Wintermute0Pamela A Silver1Department of Systems Biology, Harvard Medical School Boston MA USADepartment of Systems Biology, Harvard Medical School Boston MA USAMixed microbial communities exhibit emergent biochemical properties not found in clonal monocultures. We report a new type of synthetic genetic interaction, synthetic mutualism in trans (SMIT), in which certain pairs of auxotrophic Escherichia coli mutants complement one another's growth by cross‐feeding essential metabolites. We find significant metabolic synergy in 17% of 1035 such pairs tested, with SMIT partners identified throughout the metabolic network. Cooperative phenotypes show more growth on average by aiding the proliferation of their conjugate partner, thereby expanding the source of their own essential metabolites. We construct a quantitative, predictive, framework for describing SMIT interactions as governed by stoichiometric models of the metabolic networks of the interacting strains.https://doi.org/10.1038/msb.2010.66cooperationco‐cultureflux balance analysismetabolismmutualism |
| spellingShingle | Edwin H Wintermute Pamela A Silver Emergent cooperation in microbial metabolism Molecular Systems Biology cooperation co‐culture flux balance analysis metabolism mutualism |
| title | Emergent cooperation in microbial metabolism |
| title_full | Emergent cooperation in microbial metabolism |
| title_fullStr | Emergent cooperation in microbial metabolism |
| title_full_unstemmed | Emergent cooperation in microbial metabolism |
| title_short | Emergent cooperation in microbial metabolism |
| title_sort | emergent cooperation in microbial metabolism |
| topic | cooperation co‐culture flux balance analysis metabolism mutualism |
| url | https://doi.org/10.1038/msb.2010.66 |
| work_keys_str_mv | AT edwinhwintermute emergentcooperationinmicrobialmetabolism AT pamelaasilver emergentcooperationinmicrobialmetabolism |