C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice
Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of c...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615006337 |
| _version_ | 1818647885399457792 |
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| author | Aning Sun Gaiyan Ren Chao Deng Jingjing Zhang Xiaoping Luo Xiaojun Wu Sridhar Mani Wei Dou Zhengtao Wang |
| author_facet | Aning Sun Gaiyan Ren Chao Deng Jingjing Zhang Xiaoping Luo Xiaojun Wu Sridhar Mani Wei Dou Zhengtao Wang |
| author_sort | Aning Sun |
| collection | DOAJ |
| description | Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-4 after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-1 receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways. |
| first_indexed | 2024-12-17T01:09:38Z |
| format | Article |
| id | doaj.art-2af5cd42a48344e78cef965966f3b16f |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T01:09:38Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-2af5cd42a48344e78cef965966f3b16f2022-12-21T22:09:11ZengElsevierJournal of Functional Foods1756-46462016-03-0121418430C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in miceAning Sun0Gaiyan Ren1Chao Deng2Jingjing Zhang3Xiaoping Luo4Xiaojun Wu5Sridhar Mani6Wei Dou7Zhengtao Wang8Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaShanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaShanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaShanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaShanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaShanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaDepartments of Medicine and Genetics, Albert Einstein College of Medicine, NY 10461, USAShanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Tel.: +86 21 51322498; fax: +86 21 51322515.Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Tel.: +86 21 51322498; fax: +86 21 51322515.Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-4 after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-1 receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.http://www.sciencedirect.com/science/article/pii/S1756464615006337DSS-induced colitisTLR4NF-kBMAPKOrientin |
| spellingShingle | Aning Sun Gaiyan Ren Chao Deng Jingjing Zhang Xiaoping Luo Xiaojun Wu Sridhar Mani Wei Dou Zhengtao Wang C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice Journal of Functional Foods DSS-induced colitis TLR4 NF-kB MAPK Orientin |
| title | C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice |
| title_full | C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice |
| title_fullStr | C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice |
| title_full_unstemmed | C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice |
| title_short | C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice |
| title_sort | c glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice |
| topic | DSS-induced colitis TLR4 NF-kB MAPK Orientin |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615006337 |
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