The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1
Background Inflammation and oxidative stress (OS) are important contributors to psoriasis pathogenesis. Proanthocyanidins (PCs) have anti-inflammatory and anti-oxidative activities. Previously, we discovered that PCs alleviated psoriasis-like mice symptoms, likely via mitigating inflammation and OS...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2022.2123841 |
| _version_ | 1811206900912488448 |
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| author | Yangmeng Zhao Yuxin Xie Xiaolong Li Jing Song Menglu Guo Dehai Xian Jianqiao Zhong |
| author_facet | Yangmeng Zhao Yuxin Xie Xiaolong Li Jing Song Menglu Guo Dehai Xian Jianqiao Zhong |
| author_sort | Yangmeng Zhao |
| collection | DOAJ |
| description | Background Inflammation and oxidative stress (OS) are important contributors to psoriasis pathogenesis. Proanthocyanidins (PCs) have anti-inflammatory and anti-oxidative activities. Previously, we discovered that PCs alleviated psoriasis-like mice symptoms, likely via mitigating inflammation and OS damage.Objective To elucidate the protective mechanism underlying PCs against the damage of TNF-ɑ-induced psoriasis-like cell models.Methods Psoriasis-like cell models were established with 7.5 ng/mL TNF-ɑ and then subjected to different-concentrations PCs treatment. Finally, inflammatory and oxidative parameters were determined. Besides, LY294002 (PI3K inhibitor) and ZnPP (HO-1 inhibitor) were employed to investigate the roles of PI3K/AKT and HO-1 in PCs against psoriasis-like cell models.Results After TNF-α treatment, cells organized tightly and proliferated greatly (P<0.01); HO-1 expression dropped obviously, along with the increased OS/inflammatory indicators and the decreased antioxidants (P<0.05); consequently, psoriasis-like cell models were well established. In the presence of PCs, nevertheless, the proliferation rate and number of psoriasis-like cells evidently decreased (P<0.01), accompanied with enhanced HO-1 and antioxidants, and lowered OS/inflammatory indicators as well as phosphorylated JAK2/STAT3/PI3/AKT (P<0.01). Similar changes appeared after LY294002 pretreatment, regardless of PCs or not. But after ZnPP pretreatment with or without PCs, the opposite occurred.Conclusion The study reveals that PCs can suppress psoriasis-like cell proliferation and reduce inflammatory/OS damage through PI3K/AKT inhibition and HO-1 activation, thus promising a candidate for PCs in treating psoriasis. |
| first_indexed | 2024-04-12T03:54:52Z |
| format | Article |
| id | doaj.art-2afa31c77ce24429a0b86459ce5a0ea0 |
| institution | Directory Open Access Journal |
| issn | 1351-0002 1743-2928 |
| language | English |
| last_indexed | 2024-04-12T03:54:52Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj.art-2afa31c77ce24429a0b86459ce5a0ea02022-12-22T03:48:51ZengTaylor & Francis GroupRedox Report1351-00021743-29282022-12-0127120021110.1080/13510002.2022.2123841The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1Yangmeng Zhao0Yuxin Xie1Xiaolong Li2Jing Song3Menglu Guo4Dehai Xian5Jianqiao Zhong6Department of Dermatology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of ChinaDepartment of Dermatology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of ChinaDepartment of Dermatology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of ChinaDepartment of Dermatology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of ChinaDepartment of Dermatology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of ChinaDepartment of Anatomy, Southwest Medical University, Luzhou, People’s Republic of ChinaDepartment of Dermatology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of ChinaBackground Inflammation and oxidative stress (OS) are important contributors to psoriasis pathogenesis. Proanthocyanidins (PCs) have anti-inflammatory and anti-oxidative activities. Previously, we discovered that PCs alleviated psoriasis-like mice symptoms, likely via mitigating inflammation and OS damage.Objective To elucidate the protective mechanism underlying PCs against the damage of TNF-ɑ-induced psoriasis-like cell models.Methods Psoriasis-like cell models were established with 7.5 ng/mL TNF-ɑ and then subjected to different-concentrations PCs treatment. Finally, inflammatory and oxidative parameters were determined. Besides, LY294002 (PI3K inhibitor) and ZnPP (HO-1 inhibitor) were employed to investigate the roles of PI3K/AKT and HO-1 in PCs against psoriasis-like cell models.Results After TNF-α treatment, cells organized tightly and proliferated greatly (P<0.01); HO-1 expression dropped obviously, along with the increased OS/inflammatory indicators and the decreased antioxidants (P<0.05); consequently, psoriasis-like cell models were well established. In the presence of PCs, nevertheless, the proliferation rate and number of psoriasis-like cells evidently decreased (P<0.01), accompanied with enhanced HO-1 and antioxidants, and lowered OS/inflammatory indicators as well as phosphorylated JAK2/STAT3/PI3/AKT (P<0.01). Similar changes appeared after LY294002 pretreatment, regardless of PCs or not. But after ZnPP pretreatment with or without PCs, the opposite occurred.Conclusion The study reveals that PCs can suppress psoriasis-like cell proliferation and reduce inflammatory/OS damage through PI3K/AKT inhibition and HO-1 activation, thus promising a candidate for PCs in treating psoriasis.https://www.tandfonline.com/doi/10.1080/13510002.2022.2123841Psoriasisproanthocyanidinsinflammationoxidative stress (OS)PI3KAKT |
| spellingShingle | Yangmeng Zhao Yuxin Xie Xiaolong Li Jing Song Menglu Guo Dehai Xian Jianqiao Zhong The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1 Redox Report Psoriasis proanthocyanidins inflammation oxidative stress (OS) PI3K AKT |
| title | The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1 |
| title_full | The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1 |
| title_fullStr | The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1 |
| title_full_unstemmed | The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1 |
| title_short | The protective effect of proanthocyanidins on the psoriasis-like cell models via PI3K/AKT and HO-1 |
| title_sort | protective effect of proanthocyanidins on the psoriasis like cell models via pi3k akt and ho 1 |
| topic | Psoriasis proanthocyanidins inflammation oxidative stress (OS) PI3K AKT |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2022.2123841 |
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