Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/1/51 |
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| author | Mikhail Slotvitsky Andrey Berezhnoy Serafima Scherbina Beatrisa Rimskaya Valerya Tsvelaya Victor Balashov Anton E. Efimov Igor Agapov Konstantin Agladze |
| author_facet | Mikhail Slotvitsky Andrey Berezhnoy Serafima Scherbina Beatrisa Rimskaya Valerya Tsvelaya Victor Balashov Anton E. Efimov Igor Agapov Konstantin Agladze |
| author_sort | Mikhail Slotvitsky |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials. |
| first_indexed | 2024-03-09T11:41:05Z |
| format | Article |
| id | doaj.art-2afb793f6ca641a4abaab595d36adc74 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T11:41:05Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-2afb793f6ca641a4abaab595d36adc742023-11-30T23:32:33ZengMDPI AGMicromachines2072-666X2022-12-011415110.3390/mi14010051Polymer Kernels as Compact Carriers for Suspended CardiomyocytesMikhail Slotvitsky0Andrey Berezhnoy1Serafima Scherbina2Beatrisa Rimskaya3Valerya Tsvelaya4Victor Balashov5Anton E. Efimov6Igor Agapov7Konstantin Agladze8Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya St., 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya St., 1, 123182 Moscow, RussiaMoscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, RussiaInduced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials.https://www.mdpi.com/2072-666X/14/1/51cell culturingiPSC-CMpolymerselectrophysiological couplingelectrospinning |
| spellingShingle | Mikhail Slotvitsky Andrey Berezhnoy Serafima Scherbina Beatrisa Rimskaya Valerya Tsvelaya Victor Balashov Anton E. Efimov Igor Agapov Konstantin Agladze Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes Micromachines cell culturing iPSC-CM polymers electrophysiological coupling electrospinning |
| title | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
| title_full | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
| title_fullStr | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
| title_full_unstemmed | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
| title_short | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
| title_sort | polymer kernels as compact carriers for suspended cardiomyocytes |
| topic | cell culturing iPSC-CM polymers electrophysiological coupling electrospinning |
| url | https://www.mdpi.com/2072-666X/14/1/51 |
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