Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i>
Diazotroph mutants designed using metabolic engineering to excrete surplus ammonium were used to enhance nitrogen fixation and plant growth, as the levels of nitrogen fixation attained with diazotrophs are insufficient for the plant’s needs. In this study, wild-type (A1501) and engineered ammonium-e...
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| author | Shanshan Jiang Jiang Li Qingyu Wang Changyan Yin Yuhua Zhan Yongliang Yan Min Lin Xiubin Ke |
| author_facet | Shanshan Jiang Jiang Li Qingyu Wang Changyan Yin Yuhua Zhan Yongliang Yan Min Lin Xiubin Ke |
| author_sort | Shanshan Jiang |
| collection | DOAJ |
| description | Diazotroph mutants designed using metabolic engineering to excrete surplus ammonium were used to enhance nitrogen fixation and plant growth, as the levels of nitrogen fixation attained with diazotrophs are insufficient for the plant’s needs. In this study, wild-type (A1501) and engineered ammonium-excreting (1568/pVA3) strains of nitrogen-fixing <i>Pseudomonas stutzeri</i> strains were tested in vitro based on plant growth-promoting traits, such as phosphate solubilization ability, indole acetic acid (IAA) production and nitrogenase activities, as well as ammonium excretion as affected by mannitol-mediated osmotic stress. The maize plant growth-promoting effect of the A1501 and 1568/pVA3 strains was evaluated in pots and in the field, and the <sup>15</sup>N-dilution technique was employed to assess the proportion of plant nitrogen derived from nitrogen fixation. The results demonstrate that the 1568/pVA3 strain displayed higher IAA production and nitrogenase activity than A1501 and released significant quantities of ammonium. After 50 days, in all of the conditions assayed, maize inoculated with 1568/pVA3 accumulated more plant biomass (3.3% on average) and fixed N (39.4% on average) than plants inoculated with A1501. In the field experiment, the grain yield of maize was enhanced by 5.6% or 5.9% due to the inoculation of seeds with 1568/pVA3 in the absence or presence of exogenous N fertilizer, respectively. Therefore, the engineered <i>P. stutzeri</i> strain tested in the greenhouse and field was shown to perform better than the wild-type strain with respect to maize growth parameters and biologically fixed nitrogen. |
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| spelling | doaj.art-2b047dbf280043febc0d36f2f4aed6522023-11-24T01:26:33ZengMDPI AGMicroorganisms2076-26072022-10-011010198610.3390/microorganisms10101986Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i>Shanshan Jiang0Jiang Li1Qingyu Wang2Changyan Yin3Yuhua Zhan4Yongliang Yan5Min Lin6Xiubin Ke7Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaDiazotroph mutants designed using metabolic engineering to excrete surplus ammonium were used to enhance nitrogen fixation and plant growth, as the levels of nitrogen fixation attained with diazotrophs are insufficient for the plant’s needs. In this study, wild-type (A1501) and engineered ammonium-excreting (1568/pVA3) strains of nitrogen-fixing <i>Pseudomonas stutzeri</i> strains were tested in vitro based on plant growth-promoting traits, such as phosphate solubilization ability, indole acetic acid (IAA) production and nitrogenase activities, as well as ammonium excretion as affected by mannitol-mediated osmotic stress. The maize plant growth-promoting effect of the A1501 and 1568/pVA3 strains was evaluated in pots and in the field, and the <sup>15</sup>N-dilution technique was employed to assess the proportion of plant nitrogen derived from nitrogen fixation. The results demonstrate that the 1568/pVA3 strain displayed higher IAA production and nitrogenase activity than A1501 and released significant quantities of ammonium. After 50 days, in all of the conditions assayed, maize inoculated with 1568/pVA3 accumulated more plant biomass (3.3% on average) and fixed N (39.4% on average) than plants inoculated with A1501. In the field experiment, the grain yield of maize was enhanced by 5.6% or 5.9% due to the inoculation of seeds with 1568/pVA3 in the absence or presence of exogenous N fertilizer, respectively. Therefore, the engineered <i>P. stutzeri</i> strain tested in the greenhouse and field was shown to perform better than the wild-type strain with respect to maize growth parameters and biologically fixed nitrogen.https://www.mdpi.com/2076-2607/10/10/1986biological nitrogen fixation<i>Pseudomonas</i>ammonium excretion<sup>15</sup>N-dilution methodplant growth promotion |
| spellingShingle | Shanshan Jiang Jiang Li Qingyu Wang Changyan Yin Yuhua Zhan Yongliang Yan Min Lin Xiubin Ke Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i> Microorganisms biological nitrogen fixation <i>Pseudomonas</i> ammonium excretion <sup>15</sup>N-dilution method plant growth promotion |
| title | Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i> |
| title_full | Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i> |
| title_fullStr | Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i> |
| title_full_unstemmed | Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i> |
| title_short | Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing <i>Pseudomonas</i><i>stutzeri</i> |
| title_sort | maize growth promotion by inoculation with an engineered ammonium excreting strain of nitrogen fixing i pseudomonas i i stutzeri i |
| topic | biological nitrogen fixation <i>Pseudomonas</i> ammonium excretion <sup>15</sup>N-dilution method plant growth promotion |
| url | https://www.mdpi.com/2076-2607/10/10/1986 |
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