Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892
Wheat cultivar VL Gehun 892 has shown a high level of resistance against Australian <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (Pst) pathotypes. In this study, it was crossed with Westonia, a susceptible wheat cultivar, and digenic segregation was observed in the deriv...
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MDPI AG
2022-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/2/318 |
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| author | Harbans Bariana Lakshmi Kant Naeela Qureshi Kerrie Forrest Hanif Miah Urmil Bansal |
| author_facet | Harbans Bariana Lakshmi Kant Naeela Qureshi Kerrie Forrest Hanif Miah Urmil Bansal |
| author_sort | Harbans Bariana |
| collection | DOAJ |
| description | Wheat cultivar VL Gehun 892 has shown a high level of resistance against Australian <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (Pst) pathotypes. In this study, it was crossed with Westonia, a susceptible wheat cultivar, and digenic segregation was observed in the derived population against Pst pathotype 134 E16A+Yr17+Yr27+. Single-gene recombinant inbred line (RIL) populations were developed from F<sub>3</sub> families (VL Gehun 892/Westonia#1 and VLGehun 892/Westonia#4) that showed monogenic segregations with two distinct phenotypes. Single-gene segregation against Pst pathotype 134 E16A+Yr17+Yr27+ was confirmed in both F6 RIL populations. Bulked segregant analysis using a 90K Infinium SNP array placed <i>YrVL1</i> in the short arm of chromosome 3D and <i>YrVL2</i> in the long arm of chromosome 7B. Kompetitive allele specific polymerase chain reaction (KASP) assays were developed for the SNPs linked with <i>YrVL1</i> and <i>YrVL2</i> and were mapped onto the respective populations. <i>KASP_48179</i> (0.6 cM proximal) and <i>KASP_18087</i> (2.1 cM distal) flanked <i>YrVL1</i>, whereas <i>YrVL2</i> was mapped between <i>KASP_37096</i> (1.2 cM proximal) and <i>KASP_2239</i> (3.6 cM distal). Based on their pathotypic specificities, map locations, and stages of expression, <i>YrVL1</i> and <i>YrVL2</i> were demonstrated to be unique loci and named <i>Yr66</i> and <i>Yr67</i>, respectively. Markers linked with these genes showed more than 85% polymorphism when tested on a set of 89 Australian cultivars and hence could be used for the marker-assisted selection of these genes in wheat breeding programs, following checks of parental polymorphisms. |
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| issn | 2073-4395 |
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| spelling | doaj.art-2b0486f59fbd4419969b7f4b9f449e5f2023-11-23T18:19:57ZengMDPI AGAgronomy2073-43952022-01-0112231810.3390/agronomy12020318Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892Harbans Bariana0Lakshmi Kant1Naeela Qureshi2Kerrie Forrest3Hanif Miah4Urmil Bansal5Plant Breeding Institute, School of Life Sciences, Faculty of Science, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW 2570, AustraliaPlant Breeding Institute, School of Life Sciences, Faculty of Science, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW 2570, AustraliaPlant Breeding Institute, School of Life Sciences, Faculty of Science, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW 2570, AustraliaAgriculture Victoria Research, Department of Economic Development, Jobs, Transport and Resources, AgriBio, Bundoora, VIC 3083, AustraliaPlant Breeding Institute, School of Life Sciences, Faculty of Science, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW 2570, AustraliaPlant Breeding Institute, School of Life Sciences, Faculty of Science, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW 2570, AustraliaWheat cultivar VL Gehun 892 has shown a high level of resistance against Australian <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (Pst) pathotypes. In this study, it was crossed with Westonia, a susceptible wheat cultivar, and digenic segregation was observed in the derived population against Pst pathotype 134 E16A+Yr17+Yr27+. Single-gene recombinant inbred line (RIL) populations were developed from F<sub>3</sub> families (VL Gehun 892/Westonia#1 and VLGehun 892/Westonia#4) that showed monogenic segregations with two distinct phenotypes. Single-gene segregation against Pst pathotype 134 E16A+Yr17+Yr27+ was confirmed in both F6 RIL populations. Bulked segregant analysis using a 90K Infinium SNP array placed <i>YrVL1</i> in the short arm of chromosome 3D and <i>YrVL2</i> in the long arm of chromosome 7B. Kompetitive allele specific polymerase chain reaction (KASP) assays were developed for the SNPs linked with <i>YrVL1</i> and <i>YrVL2</i> and were mapped onto the respective populations. <i>KASP_48179</i> (0.6 cM proximal) and <i>KASP_18087</i> (2.1 cM distal) flanked <i>YrVL1</i>, whereas <i>YrVL2</i> was mapped between <i>KASP_37096</i> (1.2 cM proximal) and <i>KASP_2239</i> (3.6 cM distal). Based on their pathotypic specificities, map locations, and stages of expression, <i>YrVL1</i> and <i>YrVL2</i> were demonstrated to be unique loci and named <i>Yr66</i> and <i>Yr67</i>, respectively. Markers linked with these genes showed more than 85% polymorphism when tested on a set of 89 Australian cultivars and hence could be used for the marker-assisted selection of these genes in wheat breeding programs, following checks of parental polymorphisms.https://www.mdpi.com/2073-4395/12/2/318KASP markersmarker-assisted selectionstripe rustwheat<i>Yr</i> genes |
| spellingShingle | Harbans Bariana Lakshmi Kant Naeela Qureshi Kerrie Forrest Hanif Miah Urmil Bansal Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892 Agronomy KASP markers marker-assisted selection stripe rust wheat <i>Yr</i> genes |
| title | Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892 |
| title_full | Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892 |
| title_fullStr | Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892 |
| title_full_unstemmed | Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892 |
| title_short | Identification and Characterisation of Stripe Rust Resistance Genes <i>Yr66</i> and <i>Yr67</i> in Wheat Cultivar VL Gehun 892 |
| title_sort | identification and characterisation of stripe rust resistance genes i yr66 i and i yr67 i in wheat cultivar vl gehun 892 |
| topic | KASP markers marker-assisted selection stripe rust wheat <i>Yr</i> genes |
| url | https://www.mdpi.com/2073-4395/12/2/318 |
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