Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis
Previous studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisa...
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MDPI AG
2016-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/17/10/1622 |
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| author | Lu Li Xihui Xu Chen Chen Zhenguo Shen |
| author_facet | Lu Li Xihui Xu Chen Chen Zhenguo Shen |
| author_sort | Lu Li |
| collection | DOAJ |
| description | Previous studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the GLP gene family in rice and Arabidopsis to study their functions. In total, 43 and 32 GLPs in the rice and Arabidopsis genome were identified based on a systematic analysis, respectively. The GLP genes were clustered into six clades based on phylogenetic analysis, and many stress and developmental-related cis-elements were detected in promoters of GLP genes. In addition, subcellular location and superoxide dismutase (SOD) analysis demonstrated that the random selected OsGLP genes on chromosomes 8 and 4 of rice were expressed in the cell wall with SOD activity. Overall, our results showed that tandem duplication events, especially the clusters of tandem duplication genes on chromosome 8 in rice, play a major role in expansion of the GLP family and thus increase our understanding of the role of the GLP family in abiotic/biotic stress and development. |
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| language | English |
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| publishDate | 2016-09-01 |
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| spelling | doaj.art-2b0b2aa9471848a3a4f259be0da7ab492022-12-22T03:12:50ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-09-011710162210.3390/ijms17101622ijms17101622Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and ArabidopsisLu Li0Xihui Xu1Chen Chen2Zhenguo Shen3College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaPrevious studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the GLP gene family in rice and Arabidopsis to study their functions. In total, 43 and 32 GLPs in the rice and Arabidopsis genome were identified based on a systematic analysis, respectively. The GLP genes were clustered into six clades based on phylogenetic analysis, and many stress and developmental-related cis-elements were detected in promoters of GLP genes. In addition, subcellular location and superoxide dismutase (SOD) analysis demonstrated that the random selected OsGLP genes on chromosomes 8 and 4 of rice were expressed in the cell wall with SOD activity. Overall, our results showed that tandem duplication events, especially the clusters of tandem duplication genes on chromosome 8 in rice, play a major role in expansion of the GLP family and thus increase our understanding of the role of the GLP family in abiotic/biotic stress and development.http://www.mdpi.com/1422-0067/17/10/1622GLPstandem duplicationphylogenetic analysisexpression patterncell wallSOD activity |
| spellingShingle | Lu Li Xihui Xu Chen Chen Zhenguo Shen Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis International Journal of Molecular Sciences GLPs tandem duplication phylogenetic analysis expression pattern cell wall SOD activity |
| title | Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis |
| title_full | Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis |
| title_fullStr | Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis |
| title_full_unstemmed | Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis |
| title_short | Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis |
| title_sort | genome wide characterization and expression analysis of the germin like protein family in rice and arabidopsis |
| topic | GLPs tandem duplication phylogenetic analysis expression pattern cell wall SOD activity |
| url | http://www.mdpi.com/1422-0067/17/10/1622 |
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